| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
The primary targets of spermidine-13C4 hydrochloride are the polyamine metabolic pathways. Spermidine maintains cell membrane stability, increases antioxidant enzyme activities, and improves photosystem II function and related gene expression. It is involved in cellular processes such as autophagy and aging.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, spermidine-13C4 hydrochloride is used in cell culture studies to trace polyamine metabolism. The labeled compound enables precise tracking of spermidine uptake, distribution, and metabolism in cells using mass spectrometry or NMR. It is also used to study the effects of spermidine on cell growth, autophagy, and oxidative stress responses in various cell lines. |
| ln Vivo |
In vivo, spermidine-13C4 hydrochloride is used in animal models to study polyamine metabolism and its role in aging, cancer, and age-related diseases. The labeled compound is administered to animals, and its distribution and metabolism are tracked to understand the physiological roles of polyamines. It provides insights into cellular processes and aids the development of diagnostic or therapeutic strategies.
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| Enzyme Assay |
In vitro enzyme assays for spermidine-13C4 hydrochloride measure the activity of enzymes in the polyamine biosynthesis pathway, such as ornithine decarboxylase and spermidine synthase. The labeled compound is used as a substrate or internal standard in LC-MS/MS-based assays to quantify enzyme activity and metabolite levels. The stable isotope labeling allows for precise measurement without interference from endogenous polyamines.
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| Cell Assay |
In vitro cell experiments with spermidine-13C4 hydrochloride involve culturing cells in media containing the labeled compound. Cells incorporate the labeled spermidine into cellular pools, and its metabolism is tracked using mass spectrometry. The compound is used to study the effects of spermidine on cell proliferation, apoptosis, autophagy, and gene expression. It is also used in cancer biology research to understand the role of polyamines in tumor growth.
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| Animal Protocol |
In vivo animal experiments with spermidine-13C4 hydrochloride involve administering the labeled compound to animal models, typically via oral gavage or injection. Blood, tissue, and urine samples are collected at various time points and analyzed by LC-MS/MS to measure the distribution and metabolism of the labeled spermidine. This approach is used to study polyamine metabolism in aging, cancer, and other diseases.
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| ADME/Pharmacokinetics |
As a stable isotope-labeled compound, spermidine-13C4 hydrochloride is used as an internal standard for the accurate quantification of spermidine in biological samples. The hydrochloride salt form enhances its aqueous solubility and stability. The compound has a molecular weight of approximately 258.56 g/mol (for the trihydrochloride salt) and should be stored at -20°C.
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| Toxicity/Toxicokinetics |
Toxicological data for spermidine-13C4 hydrochloride are limited. Spermidine is a naturally occurring polyamine that is generally well-tolerated at physiological concentrations. High doses may cause toxicity, but specific studies on the labeled compound have not been reported. The compound is intended for research use only and not for human consumption.
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| References | |
| Additional Infomation |
Spermidine-13C4 hydrochloride is a research-use-only compound with no approved therapeutic indications or clinical trials. Its primary application is in research focused on polyamine metabolism, cancer biology, and age-related diseases. The stable isotope labeling enables precise tracking in metabolic and biochemical studies using mass spectrometry or NMR. Spermidine hydrochloride maintains cell membrane stability and increases antioxidant enzyme activities.
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| Molecular Formula |
C413C3H16CL3N3
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|---|---|
| Molecular Weight |
251.56
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| Exact Mass |
257.101
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| CAS # |
1313734-84-7
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| Related CAS # |
Spermidine hydrochloride;334-50-9
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| PubChem CID |
71311067
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| Appearance |
White to off-white solid powder
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| LogP |
3.861
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
13
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| Complexity |
56.8
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| Defined Atom Stereocenter Count |
0
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| SMILES |
Cl[H].Cl[H].Cl[H].N([H])(C([H])([H])C([H])([H])C([H])([H])N([H])[H])[13C]([H])([H])[13C]([H])([H])[13C]([H])([H])[13C]([H])([H])N([H])[H]
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| InChi Key |
LCNBIHVSOPXFMR-GOOBRNBHSA-N
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| InChi Code |
InChI=1S/C7H19N3.3ClH/c8-4-1-2-6-10-7-3-5-9;;;/h10H,1-9H2;3*1H/i1+1,2+1,4+1,6+1;;;
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| Chemical Name |
N'-(3-aminopropyl)(1,2,3,4-13C4)butane-1,4-diamine;trihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9752 mL | 19.8760 mL | 39.7519 mL | |
| 5 mM | 0.7950 mL | 3.9752 mL | 7.9504 mL | |
| 10 mM | 0.3975 mL | 1.9876 mL | 3.9752 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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