| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Sodium pyruvate-13C3 has no pharmacological target, as it is an isotope tracer. The unlabeled sodium pyruvate is a key intermediate in several metabolic pathways, including glycolysis and the TCA cycle. It is the end product of glycolysis and serves as the primary substrate for the enzyme pyruvate dehydrogenase (PDH), which produces acetyl-CoA, linking glycolysis to the TCA cycle.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope tracer, Sodium pyruvate-13C3 is not tested for pharmacological activity. The unlabeled pyruvate has several in vitro activities: it acts as a powerful antioxidant by directly scavenging reactive oxygen species (ROS) like hydrogen peroxide. It is also a key energy source for many cell types and is a common supplement in cell culture media to support robust cell growth and metabolism. |
| ln Vivo |
In vivo, the 13C-labeled pyruvate is used to trace central carbon metabolism. The unlabeled compound is an endogenous metabolic fuel that is critical for maintaining energy homeostasis, especially in the brain and heart. It is involved in gluconeogenesis (the production of glucose) and is a precursor to alanine, lactate, and oxaloacetate. Sodium pyruvate has been studied for its potential in treating heart failure and metabolic disorders.
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| Enzyme Assay |
Sodium pyruvate-13C3 is used as an internal standard for LC-MS or GC-MS. For in vitro assays, a stock solution is typically prepared in water or a 0.1% formic acid solution. A known amount of this standard is added to biological samples (e.g., plasma, urine, tissue homogenates) before processing. After protein precipitation and centrifugation, the sample is analyzed, and the signal ratio of unlabeled pyruvate to the 13C3-labeled standard is used for quantification.
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| Cell Assay |
For in vitro cellular experiments, Sodium pyruvate-13C3 is added to cell culture media as a metabolic tracer. Cells are cultured in media containing the 13C-labeled pyruvate. At various time points (e.g., 0, 1, 3, 6, 12, 24 hours), the cells are harvested, and intracellular metabolites are extracted. The samples are then analyzed by LC-MS to track the 13C label as it moves through the TCA cycle, into lactate, or into other biosynthetic pathways.
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| Animal Protocol |
For in vivo animal experiments, Sodium pyruvate-13C3 is typically administered via a bolus or continuous intravenous (IV) infusion in rodents. Blood samples are collected at multiple time points before and after administration. At the end of the experiment, tissues such as the liver, brain, and heart may also be harvested. Samples are processed and analyzed by GC-MS or LC-MS. The distribution of the 13C label reveals real-time information about metabolic pathway activity in the whole body.
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| ADME/Pharmacokinetics |
The pharmacokinetics (PK) of Sodium pyruvate-13C3 are identical to those of unlabeled pyruvate. Pyruvate is a small, water-soluble molecule that is rapidly cleared from the bloodstream with a very short half-life (on the order of minutes). It is taken up by cells and metabolized quickly, primarily via the pyruvate dehydrogenase complex (PDH) to acetyl-CoA or via lactate dehydrogenase (LDH) to lactate. Exogenous pyruvate is not typically found in high concentrations in plasma for long periods.
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| Toxicity/Toxicokinetics |
Pyruvate is a naturally occurring and ubiquitous metabolite with very low toxicity. It is generally recognized as safe (GRAS) and is commonly used as a nutritional supplement and ingredient in health products. The 13C-labeled version is chemically identical and is considered non-toxic. Even at high concentrations, it is well-tolerated by the body, as it is a standard intermediate in essential metabolic pathways that are constantly active.
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| References |
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| Additional Infomation |
Sodium pyruvate-13C3 is not a drug but a stable isotope-labeled research tracer. Its primary use is in the field of metabolomics to map and quantify metabolic flux, providing a dynamic picture of cellular metabolism. It is also crucial in clinical research for diagnosing inborn errors of metabolism. Furthermore, it serves as a high-purity internal standard for the accurate quantification of pyruvate in various biological matrices.
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| Molecular Formula |
13C3H3NAO3
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|---|---|
| Molecular Weight |
113.02
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| Exact Mass |
113.008
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| CAS # |
142014-11-7
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| Related CAS # |
Sodium 2-oxopropanoate;113-24-6
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| PubChem CID |
16213477
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| Appearance |
White to off-white solid powder
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| Melting Point |
>300ºC(lit.)
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
7
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| Complexity |
88.2
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[13CH3][13C](=O)[13C](=O)[O-].[Na+]
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| InChi Key |
DAEPDZWVDSPTHF-HCULJTSZSA-M
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| InChi Code |
InChI=1S/C3H4O3.Na/c1-2(4)3(5)6;/h1H3,(H,5,6);/q;+1/p-1/i1+1,2+1,3+1;
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| Chemical Name |
sodium;2-oxo(1,2,3-13C3)propanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (2212.00 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 8.8480 mL | 44.2400 mL | 88.4799 mL | |
| 5 mM | 1.7696 mL | 8.8480 mL | 17.6960 mL | |
| 10 mM | 0.8848 mL | 4.4240 mL | 8.8480 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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