| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
L-Arginine-15N2 hydrochloride has no independent pharmacological target as a stable isotope tracer. The unlabeled L-arginine is a semi-essential amino acid that serves as the primary nitrogen donor for the synthesis of nitric oxide (NO) via nitric oxide synthase (NOS). NO is a potent vasodilator and signaling molecule involved in cardiovascular regulation, immune function, and neurotransmission. L-Arginine is also a precursor for creatine and agmatine.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope tracer, L-Arginine-15N2 is not tested for in vitro pharmacological activity. In cell culture studies, it is used to trace arginine metabolism, the conversion of arginine to citrulline and NO via NOS, and the urea cycle. L-Arginine is an essential supplement in many cell culture media formulations, particularly for endothelial and immune cells. The 15N label enables precise tracking of nitrogen flux. |
| ln Vivo |
L-Arginine-15N2 hydrochloride has no independent in vivo pharmacological activity as a therapeutic agent. It is used in animal and human studies as a stable isotope tracer to measure whole-body arginine flux, NO production, and urea cycle activity. L-Arginine supplementation has been studied for its potential therapeutic effects in cardiovascular disease, erectile dysfunction, and preeclampsia.
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| Enzyme Assay |
For in vitro LC-MS/MS quantification, L-Arginine-15N2 hydrochloride is dissolved in an appropriate solvent (water, 0.1% formic acid, or methanol) to prepare a stock solution (e.g., 1 mg/mL). The internal standard is added to biological samples (plasma, serum, cell lysates) at a fixed concentration (e.g., 10-500 ng/mL). Protein precipitation is performed by adding 3-5 volumes of methanol or acetonitrile containing the internal standard. After centrifugation, the supernatant is analyzed by LC-MS/MS. The analyte-to-internal standard peak area ratio is used for quantification.
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| Cell Assay |
For in vitro cellular experiments, L-Arginine-15N2 hydrochloride can be added to cell culture media as a tracer. Cells (e.g., endothelial cells, macrophages, neurons) are cultured in standard medium. The medium is replaced with arginine-free medium containing L-Arginine-15N2 (10-500 uM). After 1-24 hours, cells are harvested, and metabolites are extracted. LC-MS is used to trace the 15N label into citrulline, NO (via nitrate and nitrite), agmatine, and creatine. NOS activity can be assessed by the conversion of labeled arginine to labeled citrulline.
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| Animal Protocol |
For in vivo animal studies, L-Arginine-15N2 hydrochloride is administered to rodents via intravenous injection, intraperitoneal injection (50-200 mg/kg), or oral gavage. Blood samples are collected at multiple time points (0, 15, 30, 60, 120, 240 minutes). At terminal time points, tissues (liver, kidney, heart, brain) are harvested. Plasma and tissue samples are analyzed by LC-MS to quantify 15N-labeled arginine and its metabolites (citrulline, ornithine, NO metabolites). For NO production, plasma nitrate/nitrite levels are measured.
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| ADME/Pharmacokinetics |
L-Arginine-15N2 hydrochloride is a stable isotope tracer and has the same pharmacokinetics as natural L-arginine. L-Arginine is absorbed from the small intestine, distributed to all tissues, and has a plasma half-life of approximately 30-90 minutes. It is metabolized primarily in the liver (urea cycle) and in endothelial cells (NOS pathway). L-Arginine is also converted to creatine in the kidney and liver. The 15N label enables precise tracking of nitrogen atoms through these pathways.
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| Toxicity/Toxicokinetics |
L-Arginine is a naturally occurring amino acid with low toxicity. The LD50 in rodents is >5,000 mg/kg. At therapeutic doses (up to 20 g/day), L-arginine is generally well-tolerated, but may cause gastrointestinal distress, hypotension, or electrolyte imbalances at very high doses. The 15N-labeled version is chemically identical and has the same safety profile. Standard laboratory safety precautions for handling amino acids apply. Not for human consumption.
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| References | |
| Additional Infomation |
L-Arginine-15N2 hydrochloride is not a drug but a 15N-labeled stable isotope research tracer. It has no approved therapeutic status. It is used as an internal standard for GC-MS or LC-MS quantification of arginine in biological samples and as a tracer for metabolic studies of the urea cycle, NO biosynthesis, and creatinine metabolism. It is also used in clinical research for studying cardiovascular disease, preeclampsia, and sickle cell disease (where NO is deficient). Available with ≥98 atom% 15N.
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| Molecular Formula |
C6H15CLN215N2O2
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|---|---|
| Molecular Weight |
212.65
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| Exact Mass |
212.082
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| CAS # |
204633-92-1
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| Related CAS # |
L-Arginine hydrochloride;1119-34-2
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| PubChem CID |
16217579
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| Appearance |
White to off-white solid powder
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| LogP |
1.354
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
13
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| Complexity |
176
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C(C[C@@H](C(=O)O)N)CN=C([15NH2])[15NH2].Cl
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| InChi Key |
KWTQSFXGGICVPE-SXTJCFDNSA-N
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| InChi Code |
InChI=1S/C6H14N4O2.ClH/c7-4(5(11)12)2-1-3-10-6(8)9;/h4H,1-3,7H2,(H,11,12)(H4,8,9,10);1H/t4-;/m0./s1/i8+1,9+1;
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| Chemical Name |
(2S)-2-amino-5-[bis(15N)(azanyl)methylideneamino]pentanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: ≥ 25 mg/mL (117.56 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7026 mL | 23.5128 mL | 47.0256 mL | |
| 5 mM | 0.9405 mL | 4.7026 mL | 9.4051 mL | |
| 10 mM | 0.4703 mL | 2.3513 mL | 4.7026 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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