| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
5-Hydroxyferulic acid acts as a substrate for the enzyme catechol-O-methyltransferase (COMT). It is a non-esterified substrate that can be methylated by COMT in the phenylpropanoid pathway, playing a role in plant metabolism. The compound's primary target is the COMT enzyme, and its activity is often studied in the context of this enzymatic reaction. |
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| ln Vitro |
Sinapic acid is the end result of the methylation of 5-hydroxyferulic acid mediated by alfalfa Caffeic acid/5-hydroxyferulic acid 3-/5-O-methyltransferase (COMT). 5-Transgenic alfalfa is downregulated by 5-hydroxyferulic acid O-methyltransferase activities (pkat mg-1 protein) in COMT stem material[1].
In cell-free enzyme assays, 5-Hydroxyferulic acid serves as a substrate for COMT. The rate of 5-hydroxyferulic acid O-methyltransferase activity is measured to assess the enzyme's function. The compound's antioxidant activity can be evaluated using standard cell-free assays such as DPPH radical scavenging, which measures its ability to neutralize free radicals. While specific cellular assay data for 5-Hydroxyferulic acid are limited, its role as a COMT substrate and its antioxidant properties suggest it can modulate cellular pathways. In cell culture, it may be used to study the effects of phenolic compounds on oxidative stress and inflammation. Its anti-inflammatory and antimicrobial properties have been investigated in various cellular models. |
| ln Vivo |
In vivo studies on 5-Hydroxyferulic acid are often related to its role as a plant metabolite. Research has been conducted on transgenic alfalfa exhibiting COMT downregulation, where the rate of 5-hydroxyferulic acid O-methyltransferase activity in the stem material was measured. These studies help to understand the compound's role in plant metabolism and its potential health benefits.
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| Enzyme Assay |
Cell-free enzyme activity assays for 5-Hydroxyferulic acid measure its role as a substrate for COMT. The assay typically involves incubating the compound with COMT enzyme and a methyl donor, such as S-adenosylmethionine (SAM). The rate of methylation is then measured, often by detecting the formation of the methylated product, sinapic acid.
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| Cell Assay |
Cellular assays for 5-Hydroxyferulic acid could be conducted using various cell lines to study its antioxidant and anti-inflammatory properties. Cells would be treated with the compound, and markers of oxidative stress, such as reactive oxygen species (ROS) levels, and inflammation, such as cytokine production, would be measured.
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| Animal Protocol |
In vivo studies on 5-Hydroxyferulic acid in animal models are not extensively detailed in the available literature. However, its role as a plant metabolite and its potential health benefits have been investigated in the context of plant biology and nutrition.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-Hydroxyferulic acid are not extensively characterized. As a phenolic acid, it is likely absorbed and metabolized, but detailed parameters such as bioavailability and half-life are not commonly reported in the available literature.
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| Toxicity/Toxicokinetics |
Preclinical toxicity data for 5-Hydroxyferulic acid are limited. As a naturally occurring phenolic compound, it is generally considered to have low toxicity. Its antioxidant properties may provide protective effects, but comprehensive toxicological studies are not detailed in the provided sources.
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| References |
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| Additional Infomation |
5-Hydroxyferulic acid is a compound in which the hydrogen atom at the 5-position of ferulic acid is replaced by a hydroxyl group. It is a hydroxycinnamic acid and a methoxycinnamic acid, and is also the conjugate acid of 5-hydroxyferulic acid esters. It has been reported that 5-hydroxyferulic acid is found in corn, barley, and several other organisms with relevant data.
5-Hydroxyferulic acid is a research compound used in studies of phenolic compounds, plant metabolism, and health benefits. It is a key intermediate in the phenylpropanoid pathway and is studied for its potential antioxidant, anti-inflammatory, and antimicrobial properties. It is not approved for clinical use. |
| Molecular Formula |
C10H10O5
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|---|---|
| Molecular Weight |
210.18
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| Exact Mass |
210.053
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| CAS # |
1782-55-4
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| Related CAS # |
(E)-5-Hydroxyferulic acid;110642-42-7
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| PubChem CID |
446834
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.44g/cm3
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| Boiling Point |
449.1ºC at 760mmHg
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| Melting Point |
182 °C
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| Flash Point |
182.8ºC
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| Vapour Pressure |
7.49E-09mmHg at 25°C
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| Index of Refraction |
1.664
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| LogP |
1.204
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
15
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| Complexity |
250
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=CC(=CC(=C1O)O)/C=C/C(=O)O
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| InChi Key |
YFXWTVLDSKSYLW-NSCUHMNNSA-N
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| InChi Code |
InChI=1S/C10H10O5/c1-15-8-5-6(2-3-9(12)13)4-7(11)10(8)14/h2-5,11,14H,1H3,(H,12,13)/b3-2+
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| Chemical Name |
(E)-3-(3,4-dihydroxy-5-methoxyphenyl)prop-2-enoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (475.78 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7578 mL | 23.7891 mL | 47.5783 mL | |
| 5 mM | 0.9516 mL | 4.7578 mL | 9.5157 mL | |
| 10 mM | 0.4758 mL | 2.3789 mL | 4.7578 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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