| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 100mg | |||
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| Targets |
Human Endogenous Metabolite
D-Fructose-6-phosphate targets the enzyme phosphofructokinase-1 (PFK-1) and fructose-1,6-bisphosphatase (FBPase). As a key metabolic intermediate, it is the substrate for PFK-1 in glycolysis and the product of FBPase in gluconeogenesis. It does not target a specific receptor. Its role is central to cellular energy metabolism, and it is involved in the regulation of these key metabolic pathways. |
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| ln Vitro |
Endogenous metabolites are those that the Kyoto Encyclopedia of Genes and Genomes has identified as products or substrates of the approximately 1900 metabolic enzymes that are encoded in human genome. Numerous of these metabolites have been shown to have harmful effects, as evidenced by the body of literature [1].
In vitro, D-Fructose-6-phosphate is used as a substrate in enzymatic assays to study the activity of phosphofructokinase-1, fructose-1,6-bisphosphatase, and other enzymes involved in carbohydrate metabolism. It is also used as a reference standard in analytical chemistry. It is not typically used for cell-based functional assays but is a critical component in biochemical studies of glycolysis and gluconeogenesis. |
| ln Vivo |
In vivo, D-Fructose-6-phosphate is a central metabolite in cellular energy metabolism. It is involved in the regulation of glycolysis and gluconeogenesis. It is an endogenous metabolite present in saliva and other tissues. Its levels are tightly regulated and reflect the metabolic state of the cell. It is not used as a therapeutic agent.
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| Enzyme Assay |
For non-cell-based assays, D-Fructose-6-phosphate is used as a substrate in enzyme activity assays. The compound is incubated with the enzyme (e.g., PFK-1 or FBPase) and cofactors, and the production of products is measured spectrophotometrically or by HPLC. It is also used as an analytical standard for the identification and quantification of metabolites in biological samples.
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| Cell Assay |
For in vitro cellular assays, D-Fructose-6-phosphate is not typically used as a pharmacological agent. It may be used in studies of cellular metabolism, where cells are treated with the compound or its precursors and the flux through glycolysis or the pentose phosphate pathway is measured. However, its primary use is as a research tool in biochemical assays.
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| Animal Protocol |
For in vivo animal studies, D-Fructose-6-phosphate is not typically administered for therapeutic purposes. It may be used in metabolic tracing studies where isotopically labeled F6P is administered to track glucose metabolism. However, its primary use is as a research tool in biochemistry and cell biology.
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| ADME/Pharmacokinetics |
D-Fructose-6-phosphate has a molecular weight of 260.14 and a molecular formula of C6H13O9P. It is a sugar phosphate. The compound is soluble in water. It should be stored at -20°C for long-term stability. It is typically supplied as a salt (e.g., sodium salt) for research use. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of D-Fructose-6-phosphate is not applicable, as it is an endogenous metabolite. It is not considered toxic. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References | |
| Additional Infomation |
Keto-D-fructose-6-phosphate is the open-chain form of D-fructose-6-phosphate. Functionally, it is related to D-fructose. It is the conjugate acid of D-fructose-6-phosphate (2-). Fructose-6-phosphate is a metabolite found in or produced by Escherichia coli (strains K12 and MG1655). It has also been reported to be present in peppers, alfalfa, and other organisms with relevant data. Fructose-6-phosphate is a metabolite found in or produced by Saccharomyces cerevisiae.
D-Fructose-6-phosphate (F6P, CAS 643-13-0) is a sugar intermediate in the glycolysis pathway and the pentose phosphate pathway. It is an endogenous metabolite present in saliva that affects cell growth and autophagy. It can be hydrolyzed by Fructose-1,6-bisphosphatase (FBPase). It is available for research purposes only. |
| Molecular Formula |
C6H13O9P
|
|---|---|
| Molecular Weight |
260.14
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| Exact Mass |
260.03
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| CAS # |
643-13-0
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| Related CAS # |
D-Fructose-6-phosphate-13C6 disodium x.hydrate
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| PubChem CID |
69507
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.936g/cm3
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| Boiling Point |
591.6ºC at 760 mmHg
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| Flash Point |
311.6ºC
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| Index of Refraction |
1.622
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| LogP |
-4.3
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
16
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| Complexity |
273
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C(C(C(C(C(=O)CO)O)O)O)OP(=O)(O)O
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| InChi Key |
GSXOAOHZAIYLCY-HSUXUTPPSA-N
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| InChi Code |
InChI=1S/C6H13O9P/c7-1-3(8)5(10)6(11)4(9)2-15-16(12,13)14/h4-7,9-11H,1-2H2,(H2,12,13,14)/t4-,5-,6-/m1/s1
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| Chemical Name |
[(2R,3R,4S)-2,3,4,6-tetrahydroxy-5-oxohexyl] dihydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8441 mL | 19.2204 mL | 38.4408 mL | |
| 5 mM | 0.7688 mL | 3.8441 mL | 7.6882 mL | |
| 10 mM | 0.3844 mL | 1.9220 mL | 3.8441 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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