| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
21-Deoxycortisol does not have a specific pharmacological target. It is an endogenous steroid metabolite and a marker for a specific enzyme deficiency. Its primary role in research is as a biomarker for congenital adrenal hyperplasia. Elevated levels of 21-Deoxycortisol in serum indicate a deficiency in the 21-hydroxylase enzyme, which is essential for cortisol synthesis. |
|---|---|
| ln Vitro |
In vitro, 21-Deoxycortisol is used as an analytical standard for the quantification of steroids in biological samples. It is used in assays to measure the activity of 21-hydroxylase and to study steroidogenesis. It is not typically used in cell-based functional assays. Its primary in vitro application is as a reference standard in analytical chemistry and endocrinology research.
|
| ln Vivo |
In vivo, 21-Deoxycortisol is an endogenous steroid that is produced in the adrenal gland. It is a marker for congenital adrenal hyperplasia. Elevated levels are indicative of 21-hydroxylase deficiency. It is not used as a therapeutic agent. Its primary relevance is in clinical diagnostics and research on adrenal disorders.
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| Enzyme Assay |
For non-cell-based assays, 21-Deoxycortisol is used as an analytical standard. Its identity and purity are confirmed using techniques such as NMR, HPLC, and mass spectrometry. It is not used in receptor binding assays. Its role is to serve as a reference standard for the quantification of this steroid in biological samples, supporting clinical diagnostics and research on adrenal disorders.
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| Cell Assay |
For in vitro cellular assays, 21-Deoxycortisol is not typically used for cell-based functional assays. It may be used in studies of steroidogenesis, where adrenal cells are cultured and the production of steroid metabolites is measured. However, its primary use is as an analytical standard and a biomarker.
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| Animal Protocol |
For in vivo animal studies, 21-Deoxycortisol is not typically administered for therapeutic or pharmacological studies. It may be used in metabolic studies to trace steroidogenesis pathways or to model adrenal disorders. However, its primary use is as a biomarker in clinical and preclinical research.
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| ADME/Pharmacokinetics |
21-Deoxycortisol has a molecular weight of 346.46 and a molecular formula of C21H30O4. It is a steroid. The compound is typically stored at -20°C for long-term stability. It is supplied with high purity (≥99%) for research use. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 21-Deoxycortisol has not been extensively reported. As an endogenous steroid, its toxicity is not a primary concern at physiological levels. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References | |
| Additional Infomation |
21-Deoxycortisol is a deoxycorticosterone with the structure 17xi-pregn-4-en-3,20-dione, with a β-hydroxyl group substituted at position 11 and an α-hydroxyl group substituted at position 17. It is a marker of adrenal virilization caused by 21-hydroxylase deficiency. It is a metabolite in both human serum and mice. It is an 11β-hydroxysteroid, a 17α-hydroxy-C21-steroid, a tertiary α-hydroxy ketone, and a deoxycorticosterone.
21-Deoxycortisol (CAS 641-77-0) is a corticosteroid metabolite of 17-hydroxyprogesterone produced in the adrenal gland. It serves as the definitive biomarker for 21-hydroxylase deficiency (21-OHD), the primary cause of congenital adrenal hyperplasia (CAH). It is used in research as a biomarker for congenital adrenal hyperplasia and in studies of steroid metabolism. It is available for research purposes only. |
| Molecular Formula |
C21H30O4
|
|---|---|
| Molecular Weight |
346.46
|
| Exact Mass |
346.214
|
| CAS # |
641-77-0
|
| Related CAS # |
21-Desoxycortisol-d4;21-Deoxycortisol-d8;2479914-04-8
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| PubChem CID |
92827
|
| Appearance |
White to off-white solid powder
|
| Density |
1.21g/cm3
|
| Boiling Point |
523.9ºC at 760 mmHg
|
| Flash Point |
284.7ºC
|
| Index of Refraction |
1.576
|
| LogP |
2.809
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
25
|
| Complexity |
667
|
| Defined Atom Stereocenter Count |
7
|
| SMILES |
CC(=O)[C@]1(CC[C@@H]2[C@@]1(C[C@@H]([C@H]3[C@H]2CCC4=CC(=O)CC[C@]34C)O)C)O
|
| InChi Key |
LCZBQMKVFQNSJR-UJPCIWJBSA-N
|
| InChi Code |
InChI=1S/C21H30O4/c1-12(22)21(25)9-7-16-15-5-4-13-10-14(23)6-8-19(13,2)18(15)17(24)11-20(16,21)3/h10,15-18,24-25H,4-9,11H2,1-3H3/t15-,16-,17-,18+,19-,20-,21-/m0/s1
|
| Chemical Name |
(8S,9S,10R,11S,13S,14S,17R)-17-acetyl-11,17-dihydroxy-10,13-dimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8863 mL | 14.4317 mL | 28.8634 mL | |
| 5 mM | 0.5773 mL | 2.8863 mL | 5.7727 mL | |
| 10 mM | 0.2886 mL | 1.4432 mL | 2.8863 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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