| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Thiocholesterol does not have a specific pharmacological receptor target. As a cholesterol analog, it can be incorporated into cell membranes and lipoproteins. It interacts with the same membrane environments as cholesterol. It is used to study the role of cholesterol in membrane structure and function, as well as in lipid metabolism and transport.
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|---|---|
| ln Vitro |
In vitro, Thiocholesterol is used as a research tool to study cholesterol metabolism and membrane dynamics. It can be incorporated into liposomes or cell membranes to investigate the effects of cholesterol analogs on membrane properties. It is also used in studies of lipid-protein interactions and as a substrate for enzymes involved in cholesterol metabolism.
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| ln Vivo |
In vivo, Thiocholesterol is used in research to study cholesterol metabolism and transport. It can be incorporated into lipoproteins and traced to study cholesterol distribution and metabolism. However, comprehensive in vivo pharmacological studies are limited. The compound is primarily used as a research tool rather than for therapeutic applications.
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| Enzyme Assay |
For non-cell-based assays, Thiocholesterol can be used as a standard in lipid analysis. Its identity and purity are confirmed using techniques such as TLC, HPLC, and mass spectrometry. It can be incorporated into liposomes or model membranes for studies of membrane biophysics. It is not used in standard receptor binding assays.
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| Cell Assay |
For in vitro cellular assays, Thiocholesterol can be incorporated into cell membranes by adding it to cell culture media. Cells are treated with the compound, and its incorporation into membranes is assessed. The effects of the cholesterol analog on membrane fluidity, lipid rafts, and protein function can be studied. It can also be used to study cholesterol uptake and efflux in cells.
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| Animal Protocol |
For in vivo animal studies, Thiocholesterol can be administered to rodents via oral gavage or intravenous injection. It can be incorporated into lipoproteins and traced to study cholesterol metabolism and transport. Tissue samples are collected to analyze the distribution and metabolism of the compound. Dosing regimens vary depending on the specific study objectives.
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| ADME/Pharmacokinetics |
Thiocholesterol has a molecular weight of 402.72 g/mol and a molecular formula of C27H46S. It is a cholesterol analog. The compound is soluble in organic solvents. It should be stored at -20°C for long-term stability. It is for research use only and is not intended for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of Thiocholesterol has not been extensively reported. As a cholesterol analog, it is expected to have low toxicity. The compound is for research use only and is not intended for human consumption. Standard safety precautions should be observed when handling the compound.
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| References | |
| Additional Infomation |
Thiocholesterol (5-cholestene-3β-thiol, CAS 1249-81-6) is a cholesterol analog in which the 3β-hydroxyl group is replaced by a thiol group. It is used as a research tool to study cholesterol metabolism, membrane dynamics, and lipid-protein interactions. It is available for research purposes only.
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| Molecular Formula |
C27H46S
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|---|---|
| Molecular Weight |
402.72
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| Exact Mass |
402.332
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| CAS # |
1249-81-6
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| PubChem CID |
65075
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| Appearance |
Typically exists as solid at room temperature
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| Density |
0.98g/cm3
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| Boiling Point |
482.4ºC at 760mmHg
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| Melting Point |
97-99ºC(lit.)
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| Flash Point |
224ºC
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| Vapour Pressure |
5.38E-09mmHg at 25°C
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| Index of Refraction |
1.53
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| LogP |
8.326
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
28
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| Complexity |
591
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| Defined Atom Stereocenter Count |
8
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| SMILES |
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@H](CC[C@]4(C)[C@H]3CC[C@]12C)S
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| InChi Key |
QGVQZRDQPDLHHV-DPAQBDIFSA-N
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| InChi Code |
InChI=1S/C27H46S/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1
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| Chemical Name |
(3S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthrene-3-thiol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4831 mL | 12.4156 mL | 24.8311 mL | |
| 5 mM | 0.4966 mL | 2.4831 mL | 4.9662 mL | |
| 10 mM | 0.2483 mL | 1.2416 mL | 2.4831 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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