| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
No specific drug target; serves as a stable isotope-labeled internal standard for auxin quantification. In plants, IAA binds to TIR1/AFB receptors and activates auxin-responsive gene expression.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
As a stable isotope-labeled compound, 3-Indoleacetic acid-d7 exhibits identical chemical properties to unlabeled 3-indoleacetic acid. Unlabeled 3-indoleacetic acid (IAA) is the most common natural plant growth hormone of the auxin class. In vitro, it can be added to plant cell culture medium to induce plant cell elongation and division at concentrations of 0.1-10 uM. |
| ln Vivo |
The non-labeled 3-indoleacetic acid is a plant hormone that regulates various aspects of plant growth and development, including cell elongation, division, differentiation, tropic responses, and apical dominance. It is not a therapeutic agent for human use but is an important tool in agricultural and plant biology research.
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| Enzyme Assay |
Receptor binding assays are not applicable for an analytical internal standard. Standard analytical protocols for 3-Indoleacetic acid-d7 involve LC-MS/MS or GC-MS quantification of IAA in plant tissues. Samples are homogenized and extracted with organic solvents (e.g., methanol or acetonitrile). The extract is purified by solid-phase extraction (SPE), derivatized for GC-MS if needed, and analyzed. The labeled compound is used as an internal standard for accurate quantification.
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| Cell Assay |
For plant cell culture studies, 3-Indoleacetic acid-d7 can be added to culture medium at concentrations of 0.1-10 uM as a tracer. Plant cells (e.g., Arabidopsis thaliana suspension cultures) are incubated for 1-48 hours. Cells are harvested, and labeled IAA and its metabolites are extracted and analyzed by LC-MS/MS to study auxin metabolism, transport, and signaling pathways.
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| Animal Protocol |
In vivo studies using 3-Indoleacetic acid-d7 are conducted in intact plants such as Arabidopsis thaliana or crop species. Plants are treated with the labeled compound via root application, foliar spray, or infiltration. Tissue samples (roots, shoots, leaves) are collected at multiple time points (0-24 hours). IAA and its conjugates are extracted and quantified by LC-MS/MS to study auxin distribution, metabolism, and turnover.
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| ADME/Pharmacokinetics |
As an analytical internal standard, pharmacokinetic properties in animals are not applicable. The non-labeled IAA is an endogenous plant hormone not normally produced in animals. However, IAA can be produced by gut microbiota from dietary tryptophan and has been detected in human urine. It is rapidly metabolized and excreted in mammals. No significant pharmacokinetic studies have been performed.
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| Toxicity/Toxicokinetics |
No toxicity data are specifically reported for 3-Indoleacetic acid-d7 as it is an analytical standard. The non-labeled IAA is generally considered to have low toxicity in animals. However, at very high doses, IAA may cause mild toxicity. In plants, IAA is a naturally occurring hormone and is not toxic at physiological concentrations. Standard laboratory safety precautions should be followed.
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| References | |
| Additional Infomation |
3-Indoleacetic acid-d7 is a stable isotope-labeled compound used exclusively for plant biology research. It is an essential internal standard for the accurate quantification of endogenous auxin levels in plant tissues using mass spectrometry. This compound is not a therapeutic agent and has no approved clinical indications for human use. It has been cited as an internal standard for hormone quantification by HPLC-ESI-MS/MS and is widely used in studies of plant development, hormone signaling, and agricultural biotechnology.
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| Molecular Formula |
C10H2D7NO2
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|---|---|
| Molecular Weight |
182.23
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| Exact Mass |
182.107
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| CAS # |
1173020-21-7
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| Related CAS # |
3-Indoleacetic acid;87-51-4
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| PubChem CID |
71309227
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
415.0±20.0 °C at 760 mmHg
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| Flash Point |
204.8±21.8 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.694
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| LogP |
1.43
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
13
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| Complexity |
205
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C1=C(C(=C2C(=C1[2H])C(=C(N2)[2H])C([2H])([2H])C(=O)O)[2H])[2H]
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| InChi Key |
SEOVTRFCIGRIMH-PYNXLSDISA-N
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| InChi Code |
InChI=1S/C10H9NO2/c12-10(13)5-7-6-11-9-4-2-1-3-8(7)9/h1-4,6,11H,5H2,(H,12,13)/i1D,2D,3D,4D,5D2,6D
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| Chemical Name |
2,2-dideuterio-2-(2,4,5,6,7-pentadeuterio-1H-indol-3-yl)acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4876 mL | 27.4379 mL | 54.8757 mL | |
| 5 mM | 1.0975 mL | 5.4876 mL | 10.9751 mL | |
| 10 mM | 0.5488 mL | 2.7438 mL | 5.4876 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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