| Size | Price | Stock | Qty |
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| Targets |
Carboxypeptidase B targets the C-terminal basic amino acids (arginine and lysine) of peptides and proteins. As an enzyme, it does not have a receptor target but rather acts on peptide substrates. It is found in pancreatic secretions and is involved in activating digestive enzymes and modulating inflammation. It has potential therapeutic applications in treating conditions like pancreatitis, cancer, and wound healing.
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| ln Vitro |
In vitro, Carboxypeptidase B (MS grade) is used for the specific cleavage of C-terminal basic amino acids from peptides and proteins. It is used in proteomics and mass spectrometry applications for protein digestion and peptide mapping. The enzyme's activity is typically assessed by measuring the release of free amino acids from peptide substrates. It is used either alone or in combination with other proteases for protein analysis.
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| ln Vivo |
In vivo, Carboxypeptidase B is found in pancreatic secretions and plays a role in activating digestive enzymes and modulating inflammation. It has potential therapeutic applications in treating conditions like pancreatitis, cancer, and wound healing by influencing protein digestion, immune response, and tissue repair. However, the MS grade enzyme is primarily used for research applications rather than therapeutic purposes.
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| Enzyme Assay |
For non-cell-based assays, Carboxypeptidase B (MS grade) is used in enzyme activity assays to measure its proteolytic activity. The enzyme is incubated with a suitable peptide substrate containing a C-terminal basic amino acid, and the release of free amino acids is measured. Activity is typically expressed in units per mg of protein. The enzyme's identity and purity are confirmed using techniques such as SDS-PAGE and mass spectrometry.
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| Cell Assay |
For in vitro cellular assays, Carboxypeptidase B (MS grade) is not typically used for cell-based functional assays. Its primary application is in proteomics and mass spectrometry sample preparation for protein digestion. It may be used in studies of protein processing and maturation, where its ability to cleave C-terminal basic amino acids is utilized to study protein structure and function.
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| Animal Protocol |
For in vivo animal studies, Carboxypeptidase B (MS grade) is not typically administered for therapeutic purposes. It may be used in research studies to investigate the role of carboxypeptidase B in physiological processes such as blood coagulation, digestion, and inflammation. However, its primary use is as a research tool in biochemistry and proteomics rather than for in vivo administration.
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| ADME/Pharmacokinetics |
Carboxypeptidase B (MS grade) is sourced from E. coli and appears as a white to off-white solid. It has a specific activity of 42400 U/g protein. One unit is defined as the amount of enzyme that hydrolyzes hemoglobin to produce 1 μmol tyrosine per minute at 37°C. The enzyme has a molecular weight of approximately 610.70 and is supplied with a purity of ≥95%.
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| Toxicity/Toxicokinetics |
The toxicity profile of Carboxypeptidase B (MS grade) is not applicable, as it is an enzyme used for research purposes. Standard laboratory safety precautions should be observed when handling the enzyme. The product is for research use only and is not intended for therapeutic use.
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| References |
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| Additional Infomation |
A zinc-dependent carboxypeptidase primarily found in the digestive system. This enzyme preferentially catalyzes the cleavage of terminal lysine or arginine residues in peptide chains. It was previously classified as EC 3.4.2.2 and EC 3.4.12.3.
Carboxypeptidase B (MS grade, EC 3.4.2.2, CAS 9025-24-5) is a metalloprotease enzyme that specifically cleaves basic amino acids (arginine and lysine) from the carboxyl-terminal end of peptides and proteins. It is used in proteomics and mass spectrometry applications for protein digestion and peptide mapping. It is sourced from E. coli and is available for research purposes only. |
| Molecular Formula |
C31H38N4O7S
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|---|---|
| Molecular Weight |
610.72102
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| Exact Mass |
610.246
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| CAS # |
9025-24-5
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| Related CAS # |
Carboxypeptidase B;9025-24-5
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| PubChem CID |
3289209
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
966.7±65.0 °C at 760 mmHg
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| Flash Point |
538.4±34.3 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.595
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| LogP |
3.26
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
43
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| Complexity |
997
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| Defined Atom Stereocenter Count |
0
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| SMILES |
0
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| InChi Key |
TWURVFFNODFJBJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
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| Chemical Name |
4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6374 mL | 8.1871 mL | 16.3741 mL | |
| 5 mM | 0.3275 mL | 1.6374 mL | 3.2748 mL | |
| 10 mM | 0.1637 mL | 0.8187 mL | 1.6374 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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