| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg | |||
| Other Sizes |
| Targets |
MAPK/FAK[1]
Cell surface heparan sulfate glycosaminoglycans. Adhesamine binds selectively to heparan sulfate, inducing its clustering and activating adhesion-linked signaling pathways, including the MAPK/FAK pathway. It promotes cell adhesion, spreading, and growth. |
|---|---|
| ln Vitro |
Adhesamine (20 μg/mL; 0-30 d) enhances the survival and attachment of primary cultured hippocampus neurons in mice. Additionally, in primary cultured hippocampus neurons from mice, adhesamine causes neurite branching and fast differentiation [1]. Heparin sulfate on the cell surface (20 μg/mL; 1 d, 3 d, 4 d) stimulates FAK activation and cell adhesion. By activating MAPK, adhesamine also promotes neurite differentiation [1].
Adhesamine (20 microg/mL; 1-4 days) induces cell adhesion via cell-surface heparan sulfate and activates FAK phosphorylation in mammalian cells. It activates the MAPK/FAK pathway, promotes adhesion and growth of mammalian cells, and accelerates the differentiation and improves the survival of primary cultured mouse hippocampal neurons. Adhesamine induces apparently normal cell adhesion accompanied by organized actin structures. |
| ln Vivo |
Adhesamine accelerates differentiation and improves the survival of primary cultured mouse hippocampal neurons. It promotes cell adhesion and growth in various mammalian cell types, as demonstrated in vitro. Adhesamine is used as a cell-attaching reagent for cell engineering applications. No specific in vivo efficacy studies have been reported; the compound is primarily used as an in vitro tool for cell biology research.
|
| Enzyme Assay |
Cell-free binding assays for adhesamine binding to heparan sulfate can be performed using surface plasmon resonance (SPR). Immobilized heparan sulfate is coated onto a sensor chip, and increasing concentrations of adhesamine (0.1-100 uM) are flowed over the surface in PBS buffer (pH 7.4). Binding affinity (KD) is calculated from sensorgrams. Alternatively, a competition binding assay can be performed using a labeled adhesamine analog and soluble heparan sulfate as a competitor.
|
| Cell Assay |
Mammalian cells (e.g., HeLa, CHO, NIH3T3) are seeded in culture plates pre-coated with adhesamine at various concentrations (1-50 ug/mL) or added to the culture medium. Cells are cultured for 1-4 days. Cell adhesion is quantified by counting attached cells after washing away non-adherent cells. Cell spreading and actin organization are assessed by phalloidin staining (F-actin) and fluorescence microscopy. FAK and ERK1/2 phosphorylation (activation markers) are measured by Western blot of cell lysates. Neuronal differentiation: primary mouse hippocampal neurons are cultured with adhesamine (20 ug/mL) and evaluated for neurite outgrowth and survival.
|
| Animal Protocol |
No specific in vivo animal protocols are reported for adhesamine. As a cell-attaching reagent, adhesamine is typically used for coating culture surfaces for cell culture experiments. It is not typically administered to animals for efficacy studies. If used in vivo, potential applications would involve coating biomaterial scaffolds or injection into tissues to promote cell adhesion and integration, but such studies have not been reported. Adhesamine is used in basic cell biology research.
|
| ADME/Pharmacokinetics |
No specific PK data are reported for adhesamine. The molecular weight is 670.51 (C24H32Cl4N8O2S2). Solubility: DMSO (10-50 mg/mL) and water (1 mg/mL with sonication). Storage: -20degC, powder. Since adhesamine is not typically used in vivo, PK parameters are not characterized. It is a small molecule that could potentially be absorbed if administered systemically, but this is not relevant to its typical research use as a cell culture reagent.
|
| Toxicity/Toxicokinetics |
No toxicity data are reported for adhesamine. In cell culture, adhesamine is well-tolerated at concentrations used to promote adhesion (up to 20-50 ug/mL). No overt cytotoxicity has been observed. The compound is considered safe for research applications. It is not intended for clinical use and has not undergone formal toxicological evaluation in animals. No FDA approval.
|
| References | |
| Additional Infomation |
Other information: Adhesamine (CAS 462605-73-8) is a research-grade chemical, not FDA-approved. It is a synthetic diaryldispirotripiperazine derivative that selectively binds to cell-surface heparan sulfate, activating MAPK/FAK pathway signaling and promoting cell adhesion, growth, and differentiation. It is a valuable cell-attaching reagent for cell engineering and basic cell biology research, particularly for neuronal culture. Purity ≥98%. For research use only.
|
| Molecular Formula |
C24H32CL4N8O2S2
|
|---|---|
| Molecular Weight |
670.51
|
| Exact Mass |
668.084
|
| CAS # |
462605-73-8
|
| PubChem CID |
2828084
|
| Appearance |
Off-white to light yellow solid powder
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
6
|
| Heavy Atom Count |
40
|
| Complexity |
755
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CSC1=NC(=C(C(=N1)Cl)C=O)N2CC[N+]3(CC2)CC[N+]4(CCN(CC4)C5=C(C(=NC(=N5)SC)Cl)C=O)CC3.[Cl-].[Cl-]
|
| InChi Key |
WBOWBXMVVADADA-UHFFFAOYSA-L
|
| InChi Code |
InChI=1S/C24H32Cl2N8O2S2.2ClH/c1-37-23-27-19(25)17(15-35)21(29-23)31-3-7-33(8-4-31)11-13-34(14-12-33)9-5-32(6-10-34)22-18(16-36)20(26)28-24(30-22)38-2;;/h15-16H,3-14H2,1-2H3;2*1H/q+2;;/p-2
|
| Chemical Name |
4-chloro-6-[12-(6-chloro-5-formyl-2-methylsulfanylpyrimidin-4-yl)-3,12-diaza-6,9-diazoniadispiro[5.2.59.26]hexadecan-3-yl]-2-methylsulfanylpyrimidine-5-carbaldehyde;dichloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4914 mL | 7.4570 mL | 14.9140 mL | |
| 5 mM | 0.2983 mL | 1.4914 mL | 2.9828 mL | |
| 10 mM | 0.1491 mL | 0.7457 mL | 1.4914 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
