| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Nucleic acid detection; water-soluble dye
No specific biological target has been identified for aniline blue sodium, as it functions primarily as a dye and staining reagent rather than a direct pharmacological agent. However, the compound interacts with biological molecules through ionic and hydrophobic interactions. As an acid dye, aniline blue binds to positively charged groups on proteins and other biological macromolecules, enabling its use as a stain for nucleic acids and proteins. The dye's sulfonate groups provide anionic character that facilitates binding to cationic sites on biological structures. In histological applications, the compound stains various tissue components, allowing for visualization of cellular and subcellular structures under the microscope. Its unique chemical properties allow it to bind to specific cellular components, producing color changes that enable visualization. The compound's staining properties are based on its chemical structure rather than a specific receptor-ligand interaction. |
|---|---|
| ln Vitro |
A biochemical reagent called aniline blue, sodium salt, can be utilized in life science research as an organic compound or biological substance.
In vitro, aniline blue sodium is extensively used as a biological stain for the detection of nucleic acids and proteins in electrophoresis and microscopy applications. It is used as a biological stain for nervous tissue, cells, and connective tissue. The compound is also used as a pH indicator. In plant histology, it is used in combination with safranin for tissue staining. In biochemical research, the compound serves as a reagent for studying protein-nucleic acid interactions and for visualizing biomolecules in gel electrophoresis. Its unique chemical properties allow it to bind to specific cellular components, producing color changes that enable visualization. The dye's staining properties can be optimized by adjusting concentration, pH, and incubation time to achieve specific staining patterns. |
| Enzyme Assay |
Cell-free assays involving aniline blue sodium are focused on its use as a staining reagent. Standard protocols for protein staining in electrophoresis involve preparing a staining solution of aniline blue in methanol, acetic acid, and water. Gels are incubated in the staining solution for a specified time, followed by destaining to remove excess dye. For microscopy applications, tissue sections are incubated with the dye solution, washed, and examined under the microscope. The dye's binding to nucleic acids and proteins can be quantified spectrophotometrically by measuring absorbance at the dye's characteristic wavelength. Its use as a pH indicator involves adding the dye to a solution and observing the color change.
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| Cell Assay |
Cellular assays with aniline blue sodium involve its use as a staining reagent for the visualization of cellular components. In histological staining, cells or tissue sections are incubated with the dye solution, washed, and examined under the microscope. The dye binds to specific cellular components such as nucleic acids and proteins, producing color changes that enable visualization of cellular structures. In plant histology, it is used in combination with safranin for tissue staining. The compound's staining properties can be optimized by adjusting concentration, pH, and incubation time to achieve specific staining patterns. The dye is also used as a pH indicator, allowing for the assessment of pH in various samples.
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| Animal Protocol |
Animal studies are not typically conducted with aniline blue sodium, as it is primarily a staining reagent and dye rather than a pharmacological agent. The compound is not intended for administration to animals for therapeutic purposes. However, the dye may be used in certain experimental applications such as vital staining or for tracking biological processes in animal models. The compound's use as a dye means that comprehensive in vivo studies are not typically performed, and its safety profile is primarily based on its use as a research reagent and dye. The compound's water solubility may facilitate distribution if administered, but it is not intended for systemic exposure.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for aniline blue sodium are not available, as the compound is primarily a dye and staining reagent rather than a drug candidate. With a molecular weight of 737.73 g/mol, the compound is a large molecule that would be expected to have very limited membrane permeability and oral bioavailability if administered. The compound is water-soluble, which may facilitate renal excretion if absorbed. However, comprehensive pharmacokinetic studies including absorption, distribution, metabolism, and excretion have not been performed, as the compound is not intended for therapeutic use. For research purposes, the compound is typically handled as a neat chemical and not administered to living organisms for pharmacokinetic profiling.
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| Toxicity/Toxicokinetics |
It may cause irritation; it is harmful if inhaled, swallowed, or in contact with skin.
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| Additional Infomation |
Fluorescent dyes are chemical substances that emit light when excited by light. The wavelength of the emitted light is usually longer than the wavelength of the incident light. Fluorescent dyes are substances that can cause other substances to fluoresce, such as fluorescent dyes used to label other compounds.
Aniline blue sodium is a research chemical and dye, not an approved drug. It has no clinical trial or marketing approval status for therapeutic use. The compound is a water-soluble dye extensively used as a biological stain for the detection of nucleic acids and proteins in electrophoresis and microscopy applications. It is used as a biological stain for nervous tissue, cells, and connective tissue, and as a pH indicator. In plant histology, it is used in combination with safranin for tissue staining. The compound has a molecular formula of C32H25N3Na2O9S3 and a molecular weight of 737.73 g/mol. It appears as a blue powder, is soluble in water, and slightly soluble in ethanol. The compound is for research use only and not for human use. |
| Molecular Formula |
C32H25N3NA2O9S3
|
|---|---|
| Molecular Weight |
737.73
|
| Exact Mass |
737.054
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| Elemental Analysis |
C, 52.10; H, 3.42; N, 5.70; Na, 6.23; O, 19.52; S, 13.04
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| CAS # |
28631-66-5
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| PubChem CID |
135871623
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| Appearance |
Purple to black solid powder
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| LogP |
8.972
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| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
49
|
| Complexity |
1520
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1=CC(=CC(=C1N)S(=O)(=O)[O-])C(=C2C=CC(=NC3=CC=C(C=C3)S(=O)(=O)O)C=C2)C4=CC=C(C=C4)NC5=CC=C(C=C5)S(=O)(=O)[O-].[Na+].[Na+]
|
| InChi Key |
XOSXWYQMOYSSKB-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C32H27N3O9S3.2Na/c1-20-18-23(19-30(32(20)33)47(42,43)44)31(21-2-6-24(7-3-21)34-26-10-14-28(15-11-26)45(36,37)38)22-4-8-25(9-5-22)35-27-12-16-29(17-13-27)46(39,40)41;;/h2-19,34H,33H2,1H3,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2
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| Chemical Name |
disodium;2-amino-3-methyl-5-[[4-(4-sulfonatoanilino)phenyl]-[4-(4-sulfophenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]benzenesulfonate
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| Synonyms |
Aniline Blue; 28631-66-5; 8004-91-9; Aniline Blue, sodium salt; Acid Blue 22; C.I. Acid Blue 22; Aniline Blue (spirit soluble) ,; MFCD00036130;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (135.55 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3555 mL | 6.7775 mL | 13.5551 mL | |
| 5 mM | 0.2711 mL | 1.3555 mL | 2.7110 mL | |
| 10 mM | 0.1356 mL | 0.6778 mL | 1.3555 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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