| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
4-Methylumbelliferyl sulfate potassium is a substrate for sulfatase enzymes. Upon cleavage of the sulfate group by sulfatase, the compound releases the highly fluorescent 4-methylumbelliferone, which can be detected and quantified. The compound has also been extensively studied for its pharmacological properties, particularly in the context of antihypertensive activity.
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| ln Vitro |
In study pertaining to life sciences, 4-methylumbelliferyl sulfate potassium salt is a biochemical reagent that can be utilized as an organic substance or biological material.
In vitro, 4-Methylumbelliferyl sulfate potassium is commonly used as a fluorogenic substrate to detect sulfatase activity in biochemical and biomedical research. The compound consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, the fluorescent product is released and can be quantified using fluorescence spectroscopy. It is a highly sensitive substrate for monitoring aryl sulfatase activity. |
| ln Vivo |
In vivo data for 4-Methylumbelliferyl sulfate potassium as a therapeutic agent are limited. The compound has been studied for its pharmacological properties, particularly antihypertensive activity. However, it is primarily used as a research tool for detecting sulfatase activity rather than as a directly administered drug. The compound's in vivo relevance is primarily through its use as a probe in enzyme activity studies.
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| Enzyme Assay |
For in vitro enzyme activity assays, 4-Methylumbelliferyl sulfate potassium is used as a fluorogenic substrate for sulfatase. The assay typically involves incubating the substrate with the enzyme sample in appropriate buffer (e.g., acetate buffer, pH 5.0-6.0) at 37°C for 30-60 minutes. The reaction is stopped by adding alkaline buffer (e.g., glycine-NaOH, pH 10.4), and fluorescence is measured at excitation/emission wavelengths of approximately 360/450 nm. The amount of fluorescent product released is proportional to sulfatase activity.
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| Cell Assay |
For in vitro cell-based experiments, 4-Methylumbelliferyl sulfate potassium can be used to measure sulfatase activity in cell lysates or culture supernatants. Cells are lysed in appropriate buffer, and the substrate is added to the lysate. After incubation, fluorescence is measured to quantify sulfatase activity. The compound can also be used in live cell imaging studies to monitor sulfatase activity in real time. Standard cell culture protocols involve dissolving the substrate in DMSO or aqueous buffer.
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| Animal Protocol |
In vivo animal studies using 4-Methylumbelliferyl sulfate potassium are typically conducted to assess sulfatase activity in tissues or to evaluate the compound's pharmacological properties. For antihypertensive activity studies, the compound would be administered to hypertensive animal models via appropriate routes. Tissue samples would be collected and processed for sulfatase activity measurement using the fluorogenic substrate.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 4-Methylumbelliferyl sulfate potassium are not well-characterized in the literature. The compound is water-soluble (soluble in water at 5 mg/mL) and stable in unbuffered solution for several months at -20°C. It has a melting point of 287-290°C. As a charged sulfate salt, it is expected to have limited membrane permeability. The compound is primarily used as a research tool rather than a drug candidate.
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| Toxicity/Toxicokinetics |
4-Methylumbelliferyl sulfate potassium is a research chemical and should be handled with appropriate laboratory safety precautions. As a coumarin derivative, it may pose risks of skin and eye irritation. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment (gloves, safety goggles, lab coat) and working in a well-ventilated area.
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| Additional Infomation |
4-Methylumbelliferyl sulfate potassium (CAS 15220-11-8) is primarily a research-grade biochemical reagent, not an FDA-approved pharmaceutical drug. Its primary application is as a fluorogenic substrate for the determination of sulfatase activity in biochemical and biomedical research. The compound has also been studied for its pharmacological properties, particularly antihypertensive activity. Upon cleavage by sulfatase, it releases the highly fluorescent 4-methylumbelliferone. No clinical trials or approved indications exist for this compound.
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| Molecular Formula |
C10H7KO6S
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| Molecular Weight |
294.32
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| Exact Mass |
293.96
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| CAS # |
15220-11-8
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| Related CAS # |
25892-63-1 (Parent)
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| PubChem CID |
5044226
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| Appearance |
White to off-white solid powder
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| Melting Point |
287-290ºC
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| LogP |
2.021
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
18
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| Complexity |
452
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[K+].S(=O)(=O)([O-])OC1C([H])=C([H])C2C(C([H])([H])[H])=C([H])C(=O)OC=2C=1[H]
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| InChi Key |
CSOCSPXOODWGLJ-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1
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| Chemical Name |
potassium;(4-methyl-2-oxochromen-7-yl) sulfate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 4 mg/mL (13.59 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3977 mL | 16.9883 mL | 33.9766 mL | |
| 5 mM | 0.6795 mL | 3.3977 mL | 6.7953 mL | |
| 10 mM | 0.3398 mL | 1.6988 mL | 3.3977 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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