| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| Other Sizes |
| Targets |
L-Homoserine lactone hydrochloride does not have a defined pharmacological target of its own, as it is a synthetic intermediate. However, it is used in the synthesis of bacterial quorum-sensing signaling molecules, particularly N-(3-oxododecanoyl)-L-homoserine lactone, which act as immune modulators. The compound's lactone ring and amino group provide handles for derivatization in the synthesis of biologically active compounds.
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|---|---|
| ln Vitro |
In vitro, L-Homoserine lactone hydrochloride is primarily used as a chemical reagent and synthetic intermediate. It is used in the synthesis of organoselenium chemistry intermediates and analogs of bacterial quorum-sensing signaling molecules. The compound's hydrochloride form enhances its solubility in aqueous solutions, making it ideal for various in vitro experiments. It is a key intermediate in the synthesis of the chiral herbicide L-glufosinate.
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| ln Vivo |
In vivo activity data for L-Homoserine lactone hydrochloride itself are not available, as the compound is not intended for direct in vivo administration. Rather, it is a precursor used in the synthesis of quorum-sensing signaling molecules that are subsequently evaluated as immune modulators in vivo. The compound's in vivo relevance is indirect, through the biological activities of the final compounds derived from it.
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| Enzyme Assay |
For in vitro enzyme/receptor binding studies, L-Homoserine lactone hydrochloride is not typically evaluated as a test compound itself. Instead, it serves as a reagent for the synthesis of test compounds. The compound's properties include a molecular weight of 137.57 g/mol and a molecular formula of C₄H₈ClNO₂. The lactone ring and amino group provide handles for further derivatization in medicinal chemistry and quorum sensing research.
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| Cell Assay |
For in vitro cell-based experiments, L-Homoserine lactone hydrochloride is used as an intermediate in the synthesis of compounds that are tested in cell-based assays. The compound itself is not typically evaluated in cell-based assays. Standard cell culture protocols for test compounds synthesized from this intermediate involve dissolving the final product in DMSO or aqueous buffer and diluting to working concentrations in appropriate cell culture medium.
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| Animal Protocol |
In vivo animal studies using L-Homoserine lactone hydrochloride are conducted on the final compounds synthesized from it, such as quorum-sensing signaling molecules evaluated as immune modulators. Standard in vivo protocols involve administration to rodents via appropriate routes with pharmacokinetic and immunomodulatory endpoints. Specific protocols for the intermediate compound are not documented.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-Homoserine lactone hydrochloride as a standalone compound are not characterized in the literature. The compound has a molecular weight of 137.57 g/mol, which is within the favorable range for oral bioavailability. The hydrochloride salt form enhances aqueous solubility. The compound should be stored as powder at -20°C for up to 3 years or in solvent at -80°C for up to 1 year.
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| Toxicity/Toxicokinetics |
L-Homoserine lactone hydrochloride is a research chemical and should be handled with appropriate laboratory safety precautions. As a lactone and amine salt, it may pose risks of skin and eye irritation. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment (gloves, safety goggles, lab coat) and working in a well-ventilated area.
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| References |
[1]. Koch, T., and Buchardt, O.Synthesis of L-(+)-selenomethionineSynthesis1065-1067(1993)
[2]. Chhabra, SR, Harty, C., Hooi, DSW, et al.Synthetic analogues of the bacterial signal (quorum seeing) molecule N-(3-oxododecanoyl)-L-homoserine lactone as immune modulators J. Med. Chem. 46(1) 97-104(2003) |
| Additional Infomation |
L-Homoserine lactone hydrochloride ((3S)-3-Aminodihydro-2(3H)-furanoine hydrochloride, CAS 2185-03-7) is primarily a research-grade chemical intermediate, not an FDA-approved pharmaceutical drug. Its primary applications are in organic synthesis as a key intermediate for the synthesis of the chiral herbicide L-glufosinate and in the synthesis of organoselenium chemistry intermediates and analogs of bacterial quorum-sensing signaling molecules. The compound's hydrochloride form enhances aqueous solubility for in vitro experiments. No clinical trials or approved indications exist for this compound.
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| Molecular Formula |
C4H8CLNO2
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|---|---|
| Molecular Weight |
137.56
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| Exact Mass |
137.024
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| CAS # |
2185-03-7
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| PubChem CID |
2733667
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| Appearance |
White to off-white solid powder
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| Boiling Point |
257.1ºC at 760 mmHg
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| Melting Point |
221-226ºC
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| Flash Point |
120.1ºC
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| Vapour Pressure |
0.000411mmHg at 25°C
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| Index of Refraction |
-26 ° (C=0.2， H2O)
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| LogP |
0.762
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
8
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| Complexity |
91.7
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| Defined Atom Stereocenter Count |
1
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| SMILES |
Cl[H].O1C([C@]([H])(C([H])([H])C1([H])[H])N([H])[H])=O
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| InChi Key |
XBKCXPRYTLOQKS-DFWYDOINSA-N
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| InChi Code |
InChI=1S/C4H7NO2.ClH/c5-3-1-2-7-4(3)6;/h3H,1-2,5H2;1H/t3-;/m0./s1
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| Chemical Name |
(3S)-3-aminooxolan-2-one;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~727.0 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (18.17 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (18.17 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (18.17 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.2696 mL | 36.3478 mL | 72.6956 mL | |
| 5 mM | 1.4539 mL | 7.2696 mL | 14.5391 mL | |
| 10 mM | 0.7270 mL | 3.6348 mL | 7.2696 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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