| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| 250g |
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| Other Sizes |
| Targets |
Lauroylsarcosine sodium does not have a defined pharmacological target. It is an anionic detergent used for protein denaturation and membrane solubilization in molecular biology applications. The compound works by reducing the surface tension of water, allowing it to better penetrate and clean surfaces. It is used for purifying membrane proteins and facilitating cell lysis in RNA purification protocols.
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| ln Vitro |
In research pertaining to life sciences, sodium, or lauroylsarcosine, is a biochemical reagent that can be utilized as an organic compound or biological substance.
In vitro, lauroylsarcosine sodium is used as an ionic detergent to purify membrane proteins. It is commonly employed for purifying membrane proteins and facilitating cell lysis in RNA purification protocols. The compound is used as one of the components of the lysing solution in the comet assay and for lysing protoplasts in chromosomal DNA isolation. It shows toxicity to A549 cells after ALIF or SME exposure. |
| ln Vivo |
In vivo data for lauroylsarcosine sodium as a therapeutic agent are not available, as the compound is a detergent used for in vitro applications. It is not intended for in vivo administration and has no established in vivo pharmacokinetic or pharmacodynamic profile. The compound is considered safe for use in cosmetics.
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| Enzyme Assay |
For in vitro biochemical assays, lauroylsarcosine sodium is used as a detergent for protein purification and membrane solubilization. Standard protocols involve dissolving the compound in appropriate buffer at concentrations typically ranging from 0.1-5% (w/v) and adding it to samples for cell lysis or protein extraction. The compound's protein denaturing properties make it useful for solubilizing membrane proteins and disrupting protein-protein interactions.
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| Cell Assay |
For in vitro cell-based experiments, lauroylsarcosine sodium is not typically used as a test compound for therapeutic applications. It is a detergent used for cell lysis and protein extraction. The compound may be used in the preparation of cell lysates for downstream analyses such as protein quantification, western blotting, or RNA extraction. Its toxicity to A549 cells has been reported.
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| Animal Protocol |
In vivo animal studies for lauroylsarcosine sodium are not applicable, as the compound is a detergent used for in vitro applications. No animal studies have been conducted for this compound as a therapeutic agent, and it is not intended for diagnostic or therapeutic use in animals or humans.
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| ADME/Pharmacokinetics |
Metabolism / Metabolites
Acylsarcosine can be absorbed orally or through skin contact, while nitrosamines can enter the body through ingestion, inhalation, or skin contact. Once in the body, nitrosamines are metabolized by cytochrome P-450 enzymes, thus becoming carcinogens. Sarcosine, on the other hand, is metabolized to glycine by sarcosine dehydrogenase. (A2878, A2879, L1892) Pharmacokinetic data for lauroylsarcosine sodium are not available, as the compound is not a drug and is not administered to living organisms. The compound has a molecular weight of 293.4 g/mol. As a surfactant, it would be expected to have limited systemic absorption if applied topically. The compound is primarily used as a reagent rather than as a drug candidate. |
| Toxicity/Toxicokinetics |
Toxicity Summary
Acylsarcosines themselves are not toxic, but they are nitrosating agents. Nitrosating agents can decompose and/or react, leading to nitrosamine contamination. Nitrosamines, formed from secondary amines and amides in the presence of nitrite ions, are considered carcinogenic. The specific nitrosamine formed from acylsarcosine is N-nitrososarcosine. Once in the body, nitrosamines are activated by cytochrome P-450 enzymes. They are subsequently believed to exert their carcinogenic effects by forming DNA adducts at the N and O atoms. (L1889, L1890, A2878, A2879, A2880, A2881) Toxicity Data LC50 (Rat) = 50-500 mg/m3/4hr LD50: 175 mg/kg (intravenous injection, rat) (A2881) LD50: 2.1 g/kg (oral administration, mouse) (A2881) Lauroylsarcosine sodium is considered safe for use in cosmetics and has been approved for use in several countries. At higher concentrations, it can cause skin and eye irritation. The compound is for research use only and not for human therapeutic or diagnostic applications. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are available from safety data sheets. Standard safety practices include the use of personal protective equipment. |
| Additional Infomation |
Sodium lauroyl sarcosine is the sodium salt of a sarcosine acyl derivative. Sarcosine is a natural amino acid found in muscles and other body tissues. Acyl sarcosines are considered modified fatty acids, with their hydrocarbon chains separated at the α-position by an amide methyl group. They are used as surfactants/cleansers in hair conditioners and cosmetics, and can improve the wetting and penetration of topical medications. Acyl sarcosines and their sodium salts are also used in the metal surface treatment and processing industry due to their crystal modification, rust prevention, and corrosion resistance properties. (L1892, A2881)
Lauroylsarcosine sodium (Sodium lauroyl sarcosinate, Sarkosyl NL, CAS 137-16-6) is primarily a research-grade biochemical reagent and cosmetic ingredient, not an FDA-approved pharmaceutical drug. Its primary applications are as an anionic detergent for protein purification, membrane solubilization, and cell lysis in molecular biology research. The compound is also used in cosmetics as a surfactant. No clinical trials or approved therapeutic indications exist for this compound. |
| Molecular Formula |
C15H28NNAO3
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|---|---|
| Molecular Weight |
293.38
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| Exact Mass |
293.196
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| CAS # |
137-16-6
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| PubChem CID |
23668817
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| Appearance |
White to off-white solid powder
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| Density |
1.033 g/mL at 20 °C
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| Boiling Point |
100ºC
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| Melting Point |
46 °C
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| LogP |
2.115
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
20
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| Complexity |
260
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[Na+].O=C(C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])N(C([H])([H])[H])C([H])([H])C(=O)[O-]
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| InChi Key |
KSAVQLQVUXSOCR-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C15H29NO3.Na/c1-3-4-5-6-7-8-9-10-11-12-14(17)16(2)13-15(18)19;/h3-13H2,1-2H3,(H,18,19);/q;+1/p-1
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| Chemical Name |
sodium;2-[dodecanoyl(methyl)amino]acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 50 mg/mL (170.43 mM)
DMSO: 5 mg/mL (17.04 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4085 mL | 17.0427 mL | 34.0855 mL | |
| 5 mM | 0.6817 mL | 3.4085 mL | 6.8171 mL | |
| 10 mM | 0.3409 mL | 1.7043 mL | 3.4085 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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