| Size | Price | Stock | Qty |
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| 50g |
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| 100g |
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| Other Sizes |
| Targets |
5-Methylsalicylic acid's broad spectrum of biological activities includes potential antitumor effects. While the specific mechanisms are not fully elucidated, the compound's ability to interact with free radicals and possibly modulate inflammatory pathways may contribute to its antitumor properties. The compound exhibits diphenolase activity and can be used as an antioxidant.
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| ln Vitro |
In vitro, 5-methylsalicylic acid is used as a biochemical reagent and research tool. It exhibits diphenolase activity and can be used as an antioxidant. The compound has been used in analytical chemistry for the detection of p-hydroxybenzoic acid in urine samples. It shows significant cross-reactivity during fluorescent polarization immunoassay for salicylates in serum.
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| ln Vivo |
In vivo data for 5-methylsalicylic acid as a therapeutic agent are limited. The compound's potential antitumor effects have been suggested, but specific in vivo efficacy and pharmacokinetic data are not well-documented. The compound is a salicylic acid derivative and may share some pharmacological properties with salicylates, but it is primarily used as a research reagent.
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| Enzyme Assay |
For in vitro biochemical assays, 5-methylsalicylic acid is used as a reagent and standard. The compound can be used in antioxidant assays (e.g., DPPH, ABTS) to measure free radical scavenging activity. It is also used in analytical chemistry for the detection and quantification of salicylates and related compounds. The compound's diphenolase activity can be measured using standard enzyme assays with appropriate substrates.
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| Cell Assay |
For in vitro cell-based experiments, 5-methylsalicylic acid can be evaluated for potential antitumor activity. Cells are seeded in 96-well plates and treated with the compound at various concentrations for 24-72 hours. Cell viability is assessed using MTT or resazurin-based assays. The compound's ability to modulate inflammatory pathways can be assessed by measuring cytokine production in activated immune cells.
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| Animal Protocol |
In vivo animal studies for 5-methylsalicylic acid are limited. For potential antitumor or anti-inflammatory candidates derived from this scaffold, efficacy studies would typically be performed in mouse models of cancer or inflammation. Standard in vivo protocols involve administration to rodents via oral gavage or intraperitoneal injection, with appropriate endpoints. Specific protocols are not available.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-methylsalicylic acid are not extensively characterized. The compound has a molecular weight of 152.15 g/mol, which is favorable for oral bioavailability. As a salicylic acid derivative, it is likely to be absorbed and metabolized similarly to other salicylates, undergoing conjugation and renal excretion. Empirical pharmacokinetic data are not available.
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| Toxicity/Toxicokinetics |
5-Methylsalicylic acid is a research chemical and should be handled with appropriate laboratory safety precautions. As a carboxylic acid, it may cause skin and eye irritation. The compound is for research use only and not for human therapeutic or diagnostic applications. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment.
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| Additional Infomation |
5-Methylsalicylic acid (p-Cresotic acid, CAS 89-56-5) is primarily a research-grade biochemical reagent, not an FDA-approved pharmaceutical drug. Its primary applications are as a biochemical reagent for life science research, as an antioxidant, and in analytical chemistry. The compound exhibits potential antitumor effects. No clinical trials or approved therapeutic indications exist for this compound.
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| Molecular Formula |
C8H8O3
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|---|---|
| Molecular Weight |
152.15
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| Exact Mass |
152.047
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| CAS # |
89-56-5
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| PubChem CID |
6973
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
299.8±28.0 °C at 760 mmHg
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| Melting Point |
163-165 °C(lit.)
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| Flash Point |
149.3±20.5 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.600
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| LogP |
2.52
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
11
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| Complexity |
155
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(C1C(O)=CC=C(C)C=1)O
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| InChi Key |
DLGBEGBHXSAQOC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H8O3/c1-5-2-3-7(9)6(4-5)8(10)11/h2-4,9H,1H3,(H,10,11)
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| Chemical Name |
2-hydroxy-5-methylbenzoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5725 mL | 32.8623 mL | 65.7246 mL | |
| 5 mM | 1.3145 mL | 6.5725 mL | 13.1449 mL | |
| 10 mM | 0.6572 mL | 3.2862 mL | 6.5725 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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