| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Tetrazine-Amine monohydrochloride does not have a defined pharmacological target. It is a click chemistry reagent used for bioconjugation applications. The compound's mechanism of action involves the tetrazine group undergoing an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing trans-cyclooctene (TCO) groups. This bioorthogonal reaction enables covalent labeling of living cells and biomolecules.
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| ln Vitro |
In vitro, tetrazine-amine monohydrochloride is used as a click chemistry reagent for bioconjugation. It contains a tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with TCO-containing molecules. The compound can be used to covalently label living cells by cycloaddition. It is widely used in biomedical research for imaging, drug delivery, and biomolecule labeling applications.
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| ln Vivo |
In vivo data for tetrazine-amine monohydrochloride as a therapeutic agent are not available, as the compound is a click chemistry reagent used for in vitro and ex vivo applications. The compound may be used in the preparation of bioconjugates that are subsequently evaluated in vivo for imaging or therapeutic applications. However, the reagent itself has no established in vivo pharmacokinetic or pharmacodynamic profile as a drug.
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| Enzyme Assay |
For in vitro click chemistry reactions, tetrazine-amine monohydrochloride is used in iEDDA reactions with TCO-containing molecules. Standard protocols involve dissolving the compound in water or DMSO and reacting it with TCO-labeled biomolecules in appropriate buffer at room temperature for 1-24 hours. The reaction efficiency is monitored by HPLC or mass spectrometry. The compound's amine group can be further conjugated to carboxylic acids via amide bond formation.
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| Cell Assay |
For in vitro cell-based experiments, tetrazine-amine monohydrochloride is used for labeling living cells. Cells are treated with the compound or its conjugates at appropriate concentrations (typically 1-100 µM) for 1-4 hours at 37°C. Cell labeling is assessed by fluorescence microscopy or flow cytometry following reaction with TCO-labeled fluorophores. The compound's biocompatibility and cell permeability are evaluated in these studies.
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| Animal Protocol |
In vivo animal studies using tetrazine-amine monohydrochloride are conducted on the bioconjugates prepared from it, not on the reagent itself. For imaging applications, tetrazine-labeled probes are administered to rodents via intravenous injection, followed by injection of TCO-labeled imaging agents. Standard in vivo protocols involve administration of the probe, allowing time for accumulation, and then performing imaging studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for tetrazine-amine monohydrochloride are not available, as the compound is not a drug and is not administered to living organisms. The compound has a molecular weight of 223.67 g/mol and is highly water-soluble (≥100 mg/mL). As a click chemistry reagent, it is designed for conjugation reactions rather than as a drug candidate.
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| Toxicity/Toxicokinetics |
Tetrazine-Amine monohydrochloride is a research chemical and should be handled with appropriate laboratory safety precautions. As a tetrazine derivative, it may cause skin and eye irritation. The compound should be stored under appropriate conditions. Specific LD₅₀ values, acute toxicity classifications, and chronic toxicity data are not available in the public literature. Standard safety practices include the use of personal protective equipment and working in a fume hood.
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| References | |
| Additional Infomation |
Tetrazine-Amine monohydrochloride ((4-(1,2,4,5-Tetrazin-3-yl)phenyl)methanamine monohydrochloride, CAS 1416711-59-5) is primarily a research-grade click chemistry reagent, not an FDA-approved pharmaceutical drug. Its primary applications are as a tetrazine linker for covalently labeling living cells by cycloaddition and as a reagent for iEDDA reactions with TCO-containing molecules. No clinical trials or approved therapeutic indications exist for this compound.
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| Molecular Formula |
C9H10CLN5
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| Molecular Weight |
223.66
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| Exact Mass |
223.062
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| CAS # |
1416711-59-5
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| Related CAS # |
Tetrazine-Amine;1092689-33-2
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| PubChem CID |
86767671
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| Appearance |
Purple to purplish red solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
161
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=CC=C1CN)C2=NN=CN=N2.Cl
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| InChi Key |
FAAUXGMWUKVOPI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H9N5.ClH/c10-5-7-1-3-8(4-2-7)9-13-11-6-12-14-9;/h1-4,6H,5,10H2;1H
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| Chemical Name |
[4-(1,2,4,5-tetrazin-3-yl)phenyl]methanamine;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: ≥ 100 mg/mL (447.11 mM)
DMSO: 62.5 mg/mL (279.44 mM ) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4711 mL | 22.3554 mL | 44.7107 mL | |
| 5 mM | 0.8942 mL | 4.4711 mL | 8.9421 mL | |
| 10 mM | 0.4471 mL | 2.2355 mL | 4.4711 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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