| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Z-Lys-ONp hydrochloride itself is not a therapeutic agent and does not have a defined pharmacological target. Its biological application is as a synthetic substrate for enzymes, specifically serine proteases like trypsin. The compound is designed to be cleaved by these enzymes, releasing the chromophoric para-nitrophenol (pNP) group. Its "target" in a research context is the active site of the target protease, where the scissile bond is hydrolyzed. It is a tool for studying enzyme activity rather than a molecule that modulates a biological pathway for therapeutic effect.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
Z-Lys-ONp hydrochloride exhibits no intrinsic biological activity as a drug. Its value in vitro is purely as a reagent for measuring the activity of proteolytic enzymes. The compound's activity is defined by its susceptibility to enzymatic hydrolysis. For instance, it has been used as a substrate to monitor the hydrolysis reaction of trypsin via mass spectroscopy studies. The rate of hydrolysis, measured by the release of p-nitrophenol, is a direct indicator of enzyme activity, making it a standard tool for enzyme kinetics and inhibitor screening assays. |
| ln Vivo |
Z-Lys-ONp hydrochloride is not designed for in vivo applications and has no reported intrinsic in vivo activity. It is a research-grade biochemical reagent used exclusively in vitro for enzymatic assays. As a synthetic compound not intended for therapeutic use, it is not administered to animal models for testing pharmacological effects. Its role is limited to the laboratory, where it serves as a tool to study enzyme function in a controlled, cell-free environment.
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| Enzyme Assay |
The primary non-cellular assay for Z-Lys-ONp hydrochloride is a protease activity assay. In a typical protocol, the compound is dissolved in an appropriate buffer (e.g., 50 mM Tris-HCl, pH 8.0, containing 10 mM CaCl2). The reaction is initiated by adding the enzyme (e.g., trypsin) to the substrate solution. The hydrolysis of the ester bond releases para-nitrophenol, which can be monitored continuously by measuring the increase in absorbance at 405 nm using a spectrophotometer. The initial rate of absorbance change is proportional to enzyme activity. Controls include a blank without enzyme and a positive control with a known active enzyme. Kinetic parameters such as Km and Vmax can be determined by varying the substrate concentration.
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| Cell Assay |
Z-Lys-ONp hydrochloride is not used in traditional cell-based assays that measure cell viability, proliferation, or signaling. Its application is exclusively in cell-free biochemical assays to measure protease activity. It may be used in assays where a cell lysate or a purified protease is the source of enzymatic activity. In such cases, the lysate or enzyme is incubated with the substrate, and the release of p-nitrophenol is measured. This allows researchers to quantify the activity of a specific protease within a complex biological sample.
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| Animal Protocol |
Z-Lys-ONp hydrochloride is not used in in vivo animal studies. It is a research-grade biochemical reagent for in vitro use only. No animal experiments are conducted with this compound for the purpose of evaluating a therapeutic effect, as it is not a drug candidate. Its applications are strictly limited to laboratory-based enzymatic assays.
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| ADME/Pharmacokinetics |
Z-Lys-ONp hydrochloride is a chemical reagent and is not subject to pharmacokinetic (PK) evaluation. PK studies, which assess absorption, distribution, metabolism, and excretion (ADME), are performed on drug candidates, not on research substrates like this compound. Therefore, data on parameters such as Cmax, Tmax, half-life, or bioavailability are not applicable and do not exist for this product.
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| Toxicity/Toxicokinetics |
The toxicological profile of Z-Lys-ONp hydrochloride is not extensively documented, as it is not a therapeutic agent. Standard laboratory safety precautions should be observed when handling this compound. It may cause skin, eye, and respiratory tract irritation. As a general practice, appropriate personal protective equipment (gloves, goggles, lab coat) should be used, and contact with skin and eyes should be avoided. In case of accidental exposure, rinse thoroughly with water and seek medical advice. Detailed toxicity data, such as LD50 values, are generally not available for this research reagent.
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| References |
[1]. Luckose F, et al. Effects of amino acid derivatives on physical, mental, and physiological activities. Crit Rev Food Sci Nutr. 2015;55(13):1793-1144.
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| Additional Infomation |
Z-Lys-ONp hydrochloride is not a drug and has no clinical or therapeutic applications. It is a specialized biochemical tool used exclusively in research laboratories. Its primary value lies in its role as a chromogenic substrate for studying the kinetics and inhibition of serine proteases, particularly trypsin. The release of para-nitrophenol provides a simple, continuous, and quantitative measure of enzyme activity. It is a standard reagent in enzymology and is not approved by any regulatory agency for human or veterinary use.
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| Molecular Formula |
C20H24CLN3O6
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|---|---|
| Molecular Weight |
437.87
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| Exact Mass |
437.135
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| CAS # |
2179-15-9
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| Related CAS # |
4272-71-3 (Parent)
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| PubChem CID |
2724407
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.274g/cm3
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| Boiling Point |
606.3ºC at 760mmHg
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| Melting Point |
146-152ºC
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| Flash Point |
320.5ºC
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| Vapour Pressure |
6.64E-16mmHg at 25°C
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| LogP |
5.34
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
30
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| Complexity |
525
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| Defined Atom Stereocenter Count |
1
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| SMILES |
NCCCC[C@@H](C(OC1=CC=C([N+]([O-])=O)C=C1)=O)NC(OCC2=CC=CC=C2)=O.Cl
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| InChi Key |
JFZLPXVXTZKFDV-FERBBOLQSA-N
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| InChi Code |
InChI=1S/C20H23N3O6.ClH/c21-13-5-4-8-18(22-20(25)28-14-15-6-2-1-3-7-15)19(24)29-17-11-9-16(10-12-17)23(26)27;/h1-3,6-7,9-12,18H,4-5,8,13-14,21H2,(H,22,25);1H/t18-;/m0./s1
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| Chemical Name |
(4-nitrophenyl) (2S)-6-amino-2-(phenylmethoxycarbonylamino)hexanoate;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2838 mL | 11.4189 mL | 22.8378 mL | |
| 5 mM | 0.4568 mL | 2.2838 mL | 4.5676 mL | |
| 10 mM | 0.2284 mL | 1.1419 mL | 2.2838 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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