| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 1g |
|
||
| Other Sizes |
| Targets |
This compound does not have a specific therapeutic target. As a dipeptide, it is used as a model compound in biochemical research. It may have applications in the design of bioactive peptides and research into metabolic disorders. It can be used to study peptide transport and metabolism.
|
|---|---|
| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
This compound is not a therapeutic agent and does not have direct in vitro activity in a pharmacological sense. Its value is as a research tool. Amino acids and their derivatives have been commercially used as ergogenic supplements. |
| ln Vivo |
This compound is not used in in vivo studies as a therapeutic agent. It is a research chemical used as a model peptide. The final peptide product, if it were a drug candidate, would be the subject of in vivo testing. The dipeptide itself is not administered as a drug.
|
| Enzyme Assay |
In vitro assays for Pro-Leu typically involve studying its interactions with enzymes or receptors. For example, it can be used as a substrate or inhibitor in enzyme assays to study protease activity or as a ligand in binding studies to investigate receptor interactions.
|
| Cell Assay |
This compound is not used in standard cell-based assays as a therapeutic agent. Its role is as a research tool in biochemical studies. It may be used in cell culture to study peptide transport or metabolism. Its molecular weight is 228.29 g/mol, and its molecular formula is C₁₁H₂₀N₂O₃.
|
| Animal Protocol |
In vivo, Pro-Leu is not administered as a drug. It is a research chemical. It may be used in animal studies to investigate the pharmacokinetics of dipeptides or as a model compound in nutritional studies. It has a density of 1.126±0.06 g/cm³.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable as this is not a drug. It is a small dipeptide (MW 228.29 g/mol). Its properties are relevant for research use. It is a substrate for peptide transporters and can be hydrolyzed by peptidases.
|
| Toxicity/Toxicokinetics |
The toxicity of this compound is not extensively documented. As a research chemical, it should be handled with standard laboratory precautions. It is not intended for human or veterinary use. The specific target is Amino Acid Derivatives.
|
| References | |
| Additional Infomation |
Pro-Leu is a dipeptide composed of L-proline and L-leucine residues, and it is a metabolite.
L-Prolyl-L-leucine is a dipeptide used in biochemical research. It can be used in studies of protein folding, enzyme activity, and receptor interactions. It may also have applications in the design of bioactive peptides. It is not a pharmaceutical. |
| Molecular Formula |
C11H20N2O3
|
|---|---|
| Molecular Weight |
228.29
|
| Exact Mass |
228.147
|
| CAS # |
52899-07-7
|
| PubChem CID |
444109
|
| Appearance |
White to off-white solid powder
|
| Density |
1.126g/cm3
|
| Boiling Point |
457.5ºC at 760 mmHg
|
| Flash Point |
230.5ºC
|
| Index of Refraction |
1.496
|
| LogP |
1.073
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
16
|
| Complexity |
266
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
CC(C[C@H](NC([C@@H]1CCCN1)=O)C(O)=O)C
|
| InChi Key |
ZKQOUHVVXABNDG-IUCAKERBSA-N
|
| InChi Code |
InChI=1S/C11H20N2O3/c1-7(2)6-9(11(15)16)13-10(14)8-4-3-5-12-8/h7-9,12H,3-6H2,1-2H3,(H,13,14)(H,15,16)/t8-,9-/m0/s1
|
| Chemical Name |
(2S)-4-methyl-2-[[(2S)-pyrrolidine-2-carbonyl]amino]pentanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3804 mL | 21.9020 mL | 43.8039 mL | |
| 5 mM | 0.8761 mL | 4.3804 mL | 8.7608 mL | |
| 10 mM | 0.4380 mL | 2.1902 mL | 4.3804 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
