| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
As a quorum sensing molecule, N-Dodecanoyl-DL-homoserine lactone targets bacterial quorum sensing receptors such as LuxR-type transcriptional regulators. In Gram-negative bacteria, AHLs bind to LuxR-type receptors, which then activate transcription of target genes involved in virulence, biofilm formation, and other population-dependent behaviors. The compound's biological target is the bacterial quorum sensing system, making it a valuable tool for studying bacterial communication and for developing quorum sensing inhibitors. The compound does not target mammalian receptors in the classical pharmacological sense.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
The in vitro activity of N-Dodecanoyl-DL-homoserine lactone is evaluated through its ability to activate or inhibit quorum sensing in bacterial reporter strains. Typical assays use biosensor strains such as Agrobacterium tumefaciens or Chromobacterium violaceum that produce a visible readout (e.g., pigment production) in response to AHLs. The compound's activity is measured by its ability to induce reporter gene expression at various concentrations. The compound is also used to study biofilm formation and bacterial virulence. No specific IC₅₀ or EC₅₀ values are available for this compound. |
| ln Vivo |
In vivo activity of N-Dodecanoyl-DL-homoserine lactone has been studied in animal models of bacterial infection. The compound can modulate bacterial virulence in vivo by affecting quorum sensing-regulated gene expression. However, the compound is primarily used as a research tool rather than a therapeutic agent. The compound's role in vivo is to study the effects of quorum sensing on bacterial pathogenesis and host interactions.
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| Enzyme Assay |
The in vitro enzyme/receptor binding (non-cellular) experimental workflow for N-Dodecanoyl-DL-homoserine lactone typically involves binding assays with LuxR-type receptors or reporter strain assays. A typical workflow: prepare the compound in appropriate solvent (e.g., DMSO), prepare serial dilutions, and add to bacterial reporter strains. Incubate for a defined period, and measure the readout (e.g., β-galactosidase activity, pigment production). Calculate EC₅₀ values for activation. Characterization includes HPLC purity analysis (≥98%) and mass spectrometry confirmation.
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| Cell Assay |
In vitro cell-based experimental workflows for N-Dodecanoyl-DL-homoserine lactone involve bacterial cell-based assays. Bacteria are cultured in appropriate medium, treated with varying concentrations of the compound, and analyzed for quorum sensing-regulated phenotypes such as biofilm formation, pigment production, or virulence factor expression. The compound's effects on bacterial growth and viability may also be assessed.
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| Animal Protocol |
In vivo animal experimental workflows for N-Dodecanoyl-DL-homoserine lactone may involve infection models in rodents. Animals are infected with pathogenic bacteria, treated with the compound, and monitored for disease progression, bacterial load, and survival. The compound's effects on bacterial virulence and host immune responses are assessed.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of N-Dodecanoyl-DL-homoserine lactone have been partially characterized in the context of quorum sensing research. The compound is a small lipophilic molecule that can diffuse across bacterial membranes. Its half-life in biological systems is influenced by lactonase enzymes that degrade AHLs. The compound is metabolized by bacterial and host enzymes.
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| Toxicity/Toxicokinetics |
The toxicological data for N-Dodecanoyl-DL-homoserine lactone are limited. The compound is generally considered to have low toxicity at concentrations used for quorum sensing studies. Standard laboratory safety practices should be followed. The compound is intended for research use only.
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| References |
[1]. Luckose F, et al. Effects of amino acid derivatives on physical, mental, and physiological activities. Crit Rev Food Sci Nutr. 2015;55(13):1793-1144.
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| Additional Infomation |
N-Dodecanoyl-DL-homoserine lactone is an N-acyl amino acid.
N-Dodecanoyl-DL-homoserine lactone is a bacterial quorum sensing molecule belonging to the class of N-acyl homoserine lactones (AHLs). The compound is used in research to study bacterial communication and biofilm formation. Quorum sensing molecules are used by Gram-negative bacteria to regulate gene expression in response to cell density. The compound is not a drug and has no clinical trials or approvals. The compound is for research use only. |
| Molecular Formula |
C16H29NO3
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|---|---|
| Molecular Weight |
283.41
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| Exact Mass |
283.215
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| CAS # |
18627-38-8
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| PubChem CID |
11565426
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| Appearance |
Light yellow to yellow solid powder
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| Melting Point |
114-117 ℃
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| LogP |
3.729
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
20
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| Complexity |
291
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCC(=O)NC1CCOC1=O
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| InChi Key |
WILLZMOKUUPJSL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H29NO3/c1-2-3-4-5-6-7-8-9-10-11-15(18)17-14-12-13-20-16(14)19/h14H,2-13H2,1H3,(H,17,18)
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| Chemical Name |
N-(2-oxooxolan-3-yl)dodecanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (176.42 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5285 mL | 17.6423 mL | 35.2846 mL | |
| 5 mM | 0.7057 mL | 3.5285 mL | 7.0569 mL | |
| 10 mM | 0.3528 mL | 1.7642 mL | 3.5285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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