| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Sodium 2-aminoacetate (glycine) targets the central nervous system, where it acts as an inhibitory neurotransmitter. Glycine binds to glycine receptors, which are chloride channels, leading to hyperpolarization of neurons and inhibition of neurotransmission. It also acts as an allosteric regulator of NMDA receptors, enhancing the effects of glutamate. Glycine is also a precursor for the synthesis of proteins, glutathione, and other important molecules.
|
|---|---|
| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro activity of sodium glycinate is related to its role as a neurotransmitter and a nutrient. In cell-based assays, glycine can modulate neuronal excitability by activating glycine receptors and enhancing NMDA receptor function. It can also influence cellular metabolism as a precursor for glutathione and other molecules. As an amino acid derivative, it may also affect the release of anabolic hormones and fuel availability. |
| ln Vivo |
In vivo activity of glycine is well-established. It acts as an inhibitory neurotransmitter in the spinal cord and brainstem. Glycine has been studied for its potential therapeutic applications in various conditions, including schizophrenia, sleep disorders, and metabolic diseases. It is used therapeutically as a nutrient and in nutritional supplements.
|
| Enzyme Assay |
In vitro receptor binding assays for glycine could involve studying its interaction with glycine receptors or NMDA receptors. For example, radioligand binding assays using membrane preparations expressing glycine receptors could be performed. Increasing concentrations of glycine would be incubated with a radiolabeled ligand, and the displacement of the ligand would be measured. This assay would determine the affinity of glycine for its receptors.
|
| Cell Assay |
Cell-based assays for glycine could involve measuring its effects on neuronal cells. For example, cultured neurons could be treated with glycine, and changes in membrane potential or calcium influx could be measured using patch-clamp or fluorescent imaging techniques. This assay would assess the functional activity of glycine as a neurotransmitter.
|
| Animal Protocol |
In vivo animal experiments for glycine have been conducted in various models. For example, in rodent models of schizophrenia, glycine has been tested for its effects on cognitive function and social behavior. In sleep studies, glycine has been shown to improve sleep quality. These experiments help elucidate the therapeutic potential of glycine.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of glycine are well-characterized. It is absorbed from the gastrointestinal tract and distributed throughout the body. It crosses the blood-brain barrier via amino acid transporters. Glycine is metabolized primarily in the liver and kidneys. The half-life of glycine in plasma is relatively short.
|
| Toxicity/Toxicokinetics |
Toxicity Summary
It is safe at the current usage and concentration. Ingredient, concentration, and usage information can be found at: https://cir-reports.cir-safety.org The toxicity profile of glycine is generally considered to be very low, as it is a naturally occurring amino acid. High doses may cause gastrointestinal discomfort. It is generally recognized as safe (GRAS) for use in food and supplements【34†L18】. |
| References | |
| Additional Infomation |
A non-essential amino acid. It is mainly found in gelatin and silk fibroin and is used as a nutrient for therapeutic purposes. It is also a rapidly inhibitory neurotransmitter.
Sodium 2-aminoacetate (Sodium glycinate) is the sodium salt of the amino acid glycine, used as a nutrient, neurotransmitter, and buffer. It is not a drug in the conventional sense but has therapeutic applications as a nutrient. The compound is commercially available for research and other purposes. |
| Molecular Formula |
C2H4NNAO2
|
|---|---|
| Molecular Weight |
97.05
|
| Exact Mass |
97.013
|
| CAS # |
6000-44-8
|
| Related CAS # |
56-40-6 (Parent)
|
| PubChem CID |
4684308
|
| Appearance |
White to off-white solid powder
|
| Density |
1.014g/cm3
|
| Boiling Point |
240.9ºC at 760 mmHg
|
| Melting Point |
290ºC decomposes
|
| Flash Point |
99.5ºC
|
| Index of Refraction |
1.491
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
6
|
| Complexity |
46.8
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[Na+].[O-]C(C([H])([H])N([H])[H])=O
|
| InChi Key |
WUWHFEHKUQVYLF-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C2H5NO2.Na/c3-1-2(4)5;/h1,3H2,(H,4,5);/q;+1/p-1
|
| Chemical Name |
sodium 2-aminoacetate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
|---|
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 10.3040 mL | 51.5198 mL | 103.0397 mL | |
| 5 mM | 2.0608 mL | 10.3040 mL | 20.6079 mL | |
| 10 mM | 1.0304 mL | 5.1520 mL | 10.3040 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
