| Size | Price | Stock | Qty |
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| 25g |
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| Other Sizes |
| Targets |
L-Proline p-nitroanilide TFA targets the enzyme prolyl aminopeptidase (also known as proline iminopeptidase). This enzyme cleaves N-terminal proline residues from peptides and proteins. The compound serves as a chromogenic substrate for this enzyme, allowing for the measurement of its activity in vitro. Upon hydrolysis by prolyl aminopeptidase, the p-nitroanilide group is released, generating a yellow color that can be quantified spectrophotometrically. This makes it a valuable tool for studying the enzyme's function and for screening potential inhibitors.
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| ln Vitro |
In vitro activity of L-Proline p-nitroanilide TFA is characterized by its function as a chromogenic substrate for prolyl aminopeptidase. In the presence of the enzyme, the compound is cleaved, releasing p-nitroanilide, which can be detected by measuring absorbance at 405 nm. The rate of color development is proportional to the enzyme activity. This allows for the quantification of prolyl aminopeptidase activity in biological samples or for screening inhibitors. The compound does not exhibit intrinsic pharmacological activity beyond its utility as a substrate in enzymatic assays.
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| ln Vivo |
There is no reported in vivo activity for L-Proline p-nitroanilide TFA as it is not a drug substance. The compound is used exclusively in research settings as a chromogenic substrate for enzymatic assays. It is not administered to animals for pharmacological evaluation. Its role is confined to the laboratory, where it serves as a tool for studying prolyl aminopeptidase activity.
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| Enzyme Assay |
In vitro enzyme assays using L-Proline p-nitroanilide TFA are straightforward. A typical protocol involves incubating the substrate with a sample containing prolyl aminopeptidase in a suitable buffer. The reaction is monitored continuously or at a fixed time point by measuring the increase in absorbance at 405 nm, which corresponds to the release of p-nitroanilide. The enzyme activity is calculated from the rate of absorbance change. This assay is commonly used to measure prolyl aminopeptidase activity in cell lysates, tissue homogenates, or purified enzyme preparations.
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| Cell Assay |
L-Proline p-nitroanilide TFA is not typically used in cell-based assays as a test compound for pharmacological activity. However, it can be used in cell-based assays to measure cellular prolyl aminopeptidase activity. In this context, cells are lysed, and the lysate is incubated with the substrate. The release of p-nitroanilide is then measured spectrophotometrically. This approach can be used to assess the effects of compounds or treatments on cellular prolyl aminopeptidase activity.
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| Animal Protocol |
In vivo animal experiments are not performed with L-Proline p-nitroanilide TFA itself. The compound is a research chemical used exclusively as a substrate for enzymatic assays. It is not administered to animals for pharmacological evaluation. Its role is confined to the laboratory, where it serves as a tool for studying enzyme activity.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of L-Proline p-nitroanilide TFA have not been characterized as it is not a drug candidate. The compound is intended for research use only as an assay substrate. As a research reagent, its absorption, distribution, metabolism, and excretion (ADME) profile is not relevant to its intended use.
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| Toxicity/Toxicokinetics |
The toxicity profile has not been extensively studied, as it is a research chemical and not a pharmaceutical drug. The compound is classified for research use only and is not intended for human or veterinary use. Standard laboratory safety precautions should be followed when handling this compound. Specific toxicological information for this compound is not available in the literature.
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| References | |
| Additional Infomation |
L-Proline p-nitroanilide TFA is a chromogenic substrate for prolyl aminopeptidase used in enzymatic activity assays. It is not a drug and has no approved therapeutic indications or clinical trial history. The compound is commercially available from various chemical suppliers for research purposes only. Its primary application is in biochemistry and enzymology for measuring prolyl aminopeptidase activity. It is also known as H-Pro-pNA TFA.
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| Molecular Formula |
C13H14F3N3O5
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|---|---|
| Molecular Weight |
349.26
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| Exact Mass |
349.089
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| CAS # |
108321-19-3
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| PubChem CID |
71312105
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| Appearance |
White to light yellow solid powder
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| LogP |
2.843
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
24
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| Complexity |
378
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C1C[C@H](NC1)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-].C(=O)(C(F)(F)F)O
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| InChi Key |
KYRVEVYREUUAKH-PPHPATTJSA-N
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| InChi Code |
InChI=1S/C11H13N3O3.C2HF3O2/c15-11(10-2-1-7-12-10)13-8-3-5-9(6-4-8)14(16)17;3-2(4,5)1(6)7/h3-6,10,12H,1-2,7H2,(H,13,15);(H,6,7)/t10-;/m0./s1
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| Chemical Name |
(2S)-N-(4-nitrophenyl)pyrrolidine-2-carboxamide;2,2,2-trifluoroacetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8632 mL | 14.3160 mL | 28.6320 mL | |
| 5 mM | 0.5726 mL | 2.8632 mL | 5.7264 mL | |
| 10 mM | 0.2863 mL | 1.4316 mL | 2.8632 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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