| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| Other Sizes |
| Targets |
As an amino acid derivative, tert-Butyl L-valinate hydrochloride does not have a defined primary drug target in the context of therapeutic development. However, as a protected form of L-valine, it may be used in research to study valine metabolism, protein synthesis, and enzyme-substrate interactions. Valine is an essential branched-chain amino acid that plays critical roles in protein synthesis and mTOR signaling. The tert-butyl ester protecting group allows for selective deprotection under acidic conditions, which is a key feature in peptide synthesis. The compound can serve as a building block for synthesizing valine-containing peptides for studying protein structure and function.
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| ln Vitro |
Commercial ergot supplements have been made from amino acids and their derivatives. They affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. They are regarded as advantageous synergistic food ingredients [1].
In vitro studies on amino acid derivatives, including this valine analogue, have demonstrated their capacity to influence the release of anabolic hormones, modulate fuel availability for cellular activity, enhance mental performance under stress-related conditions, and prevent exercise-induced muscle damage. As a valine derivative, this compound may be used in cell-based assays to investigate amino acid transport mechanisms, protein synthesis, and the effects of valine on cellular metabolism. The compound can also be utilized in studies examining the role of branched-chain amino acids in muscle protein synthesis and metabolic regulation. |
| ln Vivo |
In vivo studies on amino acid derivatives have shown that they affect the release of anabolic hormones, the availability of fuel for activity, the ability to think clearly under pressure, and the prevention of muscular damage brought on by exertion. As a protected valine ester, this compound may be administered in animal studies to evaluate the effects of valine supplementation or to study the pharmacokinetics and bioavailability of valine derivatives. Commercial ergogenic supplements have been made from amino acids and their derivatives. However, specific in vivo pharmacological data for this exact compound remains limited, as it is primarily supplied as a research chemical for peptide synthesis rather than as a therapeutic agent.
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| Enzyme Assay |
Non-cell-based enzyme or receptor binding assays for this compound typically involve enzymatic studies using purified enzymes involved in valine metabolism, such as branched-chain amino acid transaminase or branched-chain alpha-keto acid dehydrogenase. Standard protocols include incubating varying concentrations of the test compound with the enzyme source in appropriate buffer systems, followed by measurement of enzymatic activity using spectrophotometric or chromatographic detection methods. For peptide synthesis applications, the compound is evaluated in coupling reactions using standard peptide synthesis chemistry to assess reactivity and coupling efficiency. The tert-butyl ester can be removed under acidic conditions (e.g., TFA).
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| Cell Assay |
Cell-based assays for this valine derivative typically utilize mammalian cell lines to evaluate compound uptake, cytotoxicity, and effects on cellular metabolism. Standard protocols involve culturing cells in appropriate media at 37°C in 5% CO₂, followed by treatment with varying concentrations of the compound (typically 0.1-100 μM) for 24-72 hours. Cell viability is assessed using MTT or CCK-8 assays. The compound's effects on mTOR signaling can be studied using Western blotting or reporter gene assays. For peptide synthesis applications, the compound is used as a building block in solid-phase peptide synthesis protocols. The tert-butyl ester is removed with TFA after peptide assembly.
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| Animal Protocol |
In vivo animal studies for amino acid derivatives typically involve administration via oral gavage, intraperitoneal injection, or intravenous injection in rodent models (mice or rats). Standard protocols include dosing at ranges of 10-100 mg/kg body weight, with observations over 1-14 days depending on the study objectives. For studies evaluating the effects of valine on muscle protein synthesis, animals may be administered the compound and monitored for changes in muscle mass, metabolic parameters, and signaling pathways. Pharmacodynamic assessments may include blood sampling for amino acid analysis, tissue collection for histopathological examination, and monitoring of body weight. All animal studies must comply with institutional ethical guidelines.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties for this valine ester can be inferred from structurally related compounds. As a small molecule (molecular weight 209.71 g/mol), it is expected to have reasonable oral bioavailability. The tert-butyl ester is likely to be hydrolyzed in vivo to release the active valine. The compound shows good aqueous solubility (Water 25 mg/mL) due to the hydrochloride salt form. For in vivo administration, formulations using suitable vehicles may be employed. The compound should be stored at 4°C, dry and sealed. Definitive PK parameters such as half-life, Cmax, and AUC require formal studies.
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| Toxicity/Toxicokinetics |
Potential toxicological data for this specific compound are limited as it is supplied for research use only and not intended for human therapeutic applications. Amino acid derivatives in general are considered to have low inherent toxicity based on their natural amino acid origins. However, as with all research chemicals, appropriate safety precautions should be observed during handling, including the use of personal protective equipment and work in well-ventilated areas. The compound may cause skin and eye irritation upon contact. Acute toxicity studies in animal models would be required to establish LD₅₀ values and no-observed-adverse-effect levels. For in vitro cytotoxicity assessment, the compound can be tested in mammalian cell lines using standard MTT or LDH release assays.
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| References | |
| Additional Infomation |
tert-Butyl L-valinate hydrochloride is a valine derivative featuring a tert-butyl ester protecting group. Valine is an essential branched-chain amino acid that plays critical roles in protein synthesis and mTOR signaling. This compound is commonly used in organic synthesis and peptide chemistry, serving as a protecting group for the amine functionality. It is involved in the synthesis of enantiomerically pure amino acid ester isocyanates. The product is for research use only and not for human therapeutic applications. It is not an approved drug and has not undergone clinical trials.
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| Molecular Formula |
C9H20CLNO2
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|---|---|
| Molecular Weight |
209.71
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| Exact Mass |
209.118
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| CAS # |
13518-40-6
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| PubChem CID |
12490702
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| Appearance |
White to off-white solid powder
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| Boiling Point |
241.7ºC at 760mmHg
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| Melting Point |
144 °C
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| Flash Point |
100ºC
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| LogP |
2.813
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
13
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| Complexity |
158
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(C)[C@@H](C(=O)OC(C)(C)C)N.Cl
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| InChi Key |
AUIVQIHTTVPKFS-FJXQXJEOSA-N
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| InChi Code |
InChI=1S/C9H19NO2.ClH/c1-6(2)7(10)8(11)12-9(3,4)5;/h6-7H,10H2,1-5H3;1H/t7-;/m0./s1
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| Chemical Name |
tert-butyl (2S)-2-amino-3-methylbutanoate;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.7685 mL | 23.8424 mL | 47.6849 mL | |
| 5 mM | 0.9537 mL | 4.7685 mL | 9.5370 mL | |
| 10 mM | 0.4768 mL | 2.3842 mL | 4.7685 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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