| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Soluble epoxide hydrolase (sEH).
|
|---|---|
| ln Vitro |
In cell-free systems using purified recombinant human or mouse sEH, Epoxy Fluor 7 is hydrolyzed to generate a fluorescence signal (Ex=330-360 nm, Em=450-470 nm). The assay is sensitive (detection limit in the low nM range) and provides a real-time readout of enzyme activity. Epoxy Fluor 7 is more stable in aqueous solution and offers approximately 2-fold better sensitivity than previously used colorimetric substrates such as NEPC.
|
| ln Vivo |
A standard enzyme activity assay: Prepare a 10-20 mM stock of Epoxy Fluor 7 in DMSO. Dilute to 50-200 uM in 100 mM phosphate buffer (pH 7.4) containing 0.1 mg/mL BSA. In a 96-well plate, mix 10 uL of purified sEH (0.5-10 nM) with 90 uL of substrate solution. Monitor fluorescence increase at 37degC using a plate reader with Ex=330 nm and Em=460 nm every 30 seconds for 10-60 minutes. For inhibitor studies, pre-incubate sEH with varying inhibitor concentrations for 10 min before adding Epoxy Fluor 7.
|
| Enzyme Assay |
Not applicable; Epoxy Fluor 7 is designed for cell lysates or purified enzyme systems, not for intact live cells.
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| Cell Assay |
For in vivo sEH activity assays, tissue homogenates or plasma samples can be analyzed ex vivo. After administering an sEH inhibitor to animals (e.g., mice, rats) or after disease induction (e.g., inflammation model), sacrifice the animals, collect tissues (liver, kidney, lung, blood), and prepare homogenates. Incubate the homogenates with 100 uM Epoxy Fluor 7 in phosphate buffer for 30-60 min at 37degC. Stop the reaction by adding 2 volumes of acetonitrile, centrifuge, and measure fluorescence in the supernatant (Ex/Em ~330/460 nm).
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| Animal Protocol |
Epoxy Fluor 7 has a molecular weight of ~401 g/mol. It is soluble in DMSO (prepare stock) and aqueous buffers (work at <5% DMSO). In animal experiments, if administered systemically (e.g., IP injection), Epoxy Fluor 7 is rapidly metabolized by endogenous sEH; thus, it is not typically used as an in vivo imaging agent, but rather as an ex vivo activity assay substrate. The fluorescent product 6-methoxy-2-naphthaldehyde is stable and can be extracted from tissues for quantification.
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| ADME/Pharmacokinetics |
No specific toxicity data is available for Epoxy Fluor 7. At the concentrations used for enzymatic assays (10-200 uM), no significant interference or enzyme inhibition has been reported. As a substrate, it is generally considered low-risk. Standard organic solvent handling protocols should be followed when working with DMSO solutions.
|
| Toxicity/Toxicokinetics |
Epoxy Fluor 7 is a research-grade biochemical tool for studying soluble epoxide hydrolase (sEH), a key enzyme in the metabolism of epoxyeicosatrienoic acids (EETs) which are involved in inflammation, blood pressure regulation, and pain. sEH inhibitors are being investigated as potential therapeutics for hypertension, diabetes, and inflammatory diseases. Epoxy Fluor 7 has not been approved for diagnostic or clinical use.
|
| Molecular Formula |
C23H19NO5
|
|---|---|
| Molecular Weight |
389.401
|
| Exact Mass |
389.126
|
| CAS # |
863223-43-2
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| PubChem CID |
25093347
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
|
| Boiling Point |
569.4±50.0 °C at 760 mmHg
|
| Flash Point |
244.4±20.3 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
|
| Index of Refraction |
1.622
|
| LogP |
3.84
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
8
|
| Heavy Atom Count |
29
|
| Complexity |
610
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
N#CC(OC(OCC1C(C2=CC=CC=C2)O1)=O)C1=CC2C=CC(OC)=CC=2C=C1
|
| InChi Key |
HDHFVICVWRKCHQ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C23H19NO5/c1-26-19-10-9-16-11-18(8-7-17(16)12-19)20(13-24)29-23(25)27-14-21-22(28-21)15-5-3-2-4-6-15/h2-12,20-22H,14H2,1H3
|
| Chemical Name |
[cyano-(6-methoxynaphthalen-2-yl)methyl] (3-phenyloxiran-2-yl)methyl carbonate
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5681 mL | 12.8403 mL | 25.6805 mL | |
| 5 mM | 0.5136 mL | 2.5681 mL | 5.1361 mL | |
| 10 mM | 0.2568 mL | 1.2840 mL | 2.5681 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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