| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Coumarin 314 does not target specific proteins or enzymes. Instead, it functions as a fluorescent dye with intense absorption in the visible spectrum and large solvent dependence. The dye's fluorescence properties are influenced by the local environment, making it useful for studying solvent effects and molecular interactions.
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|---|---|
| ln Vitro |
Coumarin 314 is a dye that absorbs strongly in the visible light and also has a large solvent dependence. Coumarin dyes are useful for laser dyes emitting blue-green light. Coumarin compounds having an electron donating group at the 7-position show strong light absorption and emission, whereas unsubstituted coumarin compounds hardly emit light. The dye exhibits a λmax of 436-437 nm in ethanol.
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| ln Vivo |
No significant in vivo activity data has been reported for Coumarin 314 as a therapeutic agent, as the compound is primarily utilized as a fluorescent dye for research and laser applications. The compound is intended for research use only.
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| Enzyme Assay |
In vitro assays for Coumarin 314 typically involve measuring its absorption and fluorescence properties in various solvents to study solvent effects. The dye can be used as a probe to study the polarity and viscosity of microenvironments. Its strong absorption in the visible spectrum makes it suitable for spectroscopic applications.
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| Cell Assay |
For in vitro cellular experiments, Coumarin 314 is dissolved in DMSO and diluted in cell culture medium. Cells are treated with the dye and analyzed by fluorescence microscopy or flow cytometry. The dye's fluorescence properties are influenced by the local environment, making it useful for studying cellular microenvironments.
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| Animal Protocol |
In vivo animal experiments with Coumarin 314 are not typically performed, as the compound is primarily used as a fluorescent dye for in vitro applications and laser dyes. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Coumarin 314 have not been characterized, as the compound is a research-use fluorescent dye rather than a drug candidate. The compound has a molecular weight of 313.35 g/mol and a molecular formula of C₁₈H₁₉NO₄. It has a density of 1.2389 g/ml and should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for Coumarin 314 has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
Coumarin 504 is a 7-aminocoumarin compound, belonging to the ethyl ester class. It is a fluorescent dye.
Coumarin 314 is a coumarin-based fluorescent dye that absorbs strongly in the visible spectrum and exhibits large solvent dependence. The compound is useful for laser dyes emitting blue-green light. Coumarin compounds with an electron donating group at the 7-position show strong light absorption and emission. The compound has no clinical or therapeutic applications and has not received regulatory approval. |
| Molecular Formula |
C18H19NO4
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|---|---|
| Molecular Weight |
313.35
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| Exact Mass |
313.131
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| CAS # |
55804-66-5
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| PubChem CID |
72653
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
542.5±50.0 °C at 760 mmHg
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| Melting Point |
140-144ºC
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| Flash Point |
281.9±30.1 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.630
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| LogP |
3.5
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
23
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| Complexity |
543
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCOC(=O)C1=CC2=CC3=C4C(=C2OC1=O)CCCN4CCC3
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| InChi Key |
VMJKUPWQKZFFCX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H19NO4/c1-2-22-17(20)14-10-12-9-11-5-3-7-19-8-4-6-13(15(11)19)16(12)23-18(14)21/h9-10H,2-8H2,1H3
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| Chemical Name |
ethyl 4-oxo-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraene-5-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1913 mL | 15.9566 mL | 31.9132 mL | |
| 5 mM | 0.6383 mL | 3.1913 mL | 6.3826 mL | |
| 10 mM | 0.3191 mL | 1.5957 mL | 3.1913 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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