| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
As an NO donor, D-SNAP does not target specific protein or enzyme receptors. Instead, it spontaneously releases nitric oxide under physiological conditions, which then interacts with various cellular targets including soluble guanylate cyclase, leading to downstream signaling effects.
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|---|---|
| ln Vitro |
D-SNAP can generate nitric oxide and form superoxides spontaneously under physiological conditions. The compound is often used to probe the cell stress response and stimulate calcium-independent synaptic vesicle release. SNAP has played a pivotal role in elucidating nitric oxide-mediated mechanisms in liver fibrosis, hepatic stellate cell phenotype maintenance, and S-nitrosylation pathways in breast cancer models to counteract radiation-induced oxidative stress.
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| ln Vivo |
D-SNAP has demonstrated in vivo activity as a nitric oxide donor. SNAP acts as a stable inhibitor of platelet aggregation. The compound has been used to study nitric oxide-mediated mechanisms in various disease models including liver fibrosis, hepatic stellate cell maintenance, and breast cancer.
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| Enzyme Assay |
In vitro NO release assays for D-SNAP typically involve incubating the compound in aqueous buffer at physiological pH (7.4) and measuring nitric oxide release using the Griess reaction or chemiluminescence detection. The compound spontaneously releases NO under these conditions. The rate and extent of NO release can be determined by measuring nitrite/nitrate accumulation over time.
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| Cell Assay |
For in vitro cellular experiments, D-SNAP is dissolved in DMSO or aqueous buffer and diluted in cell culture medium. Cells are treated with the compound at varying concentrations to induce NO-mediated cellular responses. The compound is often used to probe the cell stress response and stimulate calcium-independent synaptic vesicle release. NO-mediated signaling pathways can be assessed by measuring cGMP levels or protein S-nitrosylation.
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| Animal Protocol |
For in vivo animal experiments, D-SNAP is formulated in suitable vehicles and administered to animal models. The compound has been used to study NO-mediated mechanisms in liver fibrosis, hepatic stellate cell phenotype maintenance, and breast cancer models to counteract radiation-induced oxidative stress. SNAP acts as a stable inhibitor of platelet aggregation in vivo.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of D-SNAP have not been extensively characterized. The compound has a molecular weight of 220.25 g/mol and a molecular formula of C₇H₁₂N₂O₄S. The compound is supplied as a green crystalline solid and should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for D-SNAP has not been systematically evaluated. As an NO donor, the compound can generate nitric oxide and superoxides, which may have cytotoxic effects at high concentrations. The compound is intended for research use only and standard safety precautions should be observed.
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| Additional Infomation |
S-nitroso-N-acetyl-D-penicillamine is a nitroso compound, specifically N-acetyl-D-penicillamine in which the mercapto hydrogen is replaced by a nitroso group. It is a nitric oxide donor and a vasodilator. It is both a nitroso compound and a nitrosothio compound. Functionally, it is related to N-acetyl-D-penicillamine. It is a sulfur-containing alkyl thionitrite, belonging to the nitric oxide donor group.
D-SNAP (S-Nitroso-N-acetylpenicillamine) is the stereochemically pure D-isomer of the S-nitrosothiol class, functioning as a spontaneous nitric oxide donor under physiological conditions. The compound is used to probe cell stress responses, stimulate calcium-independent synaptic vesicle release, and study NO-mediated mechanisms in various disease models. The compound has no clinical or therapeutic applications and has not received regulatory approval. |
| Molecular Formula |
C7H12N2O4S
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|---|---|
| Molecular Weight |
220.24618
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| Exact Mass |
220.051
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| CAS # |
79032-48-7
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| PubChem CID |
6603945
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.4±0.1 g/cm3
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| Melting Point |
150-151ºC
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| Index of Refraction |
1.560
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| LogP |
1.13
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
14
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| Complexity |
254
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CC(=O)NC(C(=O)O)C(C)(C)SN=O
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| InChi Key |
ZIIQCSMRQKCOCT-YFKPBYRVSA-N
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| InChi Code |
InChI=1S/C7H12N2O4S/c1-4(10)8-5(6(11)12)7(2,3)14-9-13/h5H,1-3H3,(H,8,10)(H,11,12)/t5-/m0/s1
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| Chemical Name |
(2S)-2-acetamido-3-methyl-3-nitrososulfanylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5403 mL | 22.7015 mL | 45.4030 mL | |
| 5 mM | 0.9081 mL | 4.5403 mL | 9.0806 mL | |
| 10 mM | 0.4540 mL | 2.2701 mL | 4.5403 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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