| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
6-ROX hydrochloride does not target specific protein or enzyme receptors. Instead, the carboxylic acid group provides a reactive site for conjugation to amines through carbodiimide-mediated coupling reactions, enabling the attachment of the ROX fluorophore to biomolecules.
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| ln Vitro |
6-ROX hydrochloride is a fluorescent label for oligonucleotides that works as an acceptor coupled to 5-FAM and as a donor in FRET imaging. The dye exhibits strong absorption around 580 nm and emission near 605 nm, providing high signal intensity and photostability. The compound is frequently applied as a reference dye or covalent label for oligonucleotides, probes, and biomolecules.
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| ln Vivo |
No significant in vivo activity data has been reported for 6-ROX hydrochloride as a therapeutic agent, as the compound is primarily utilized as a fluorescent labeling reagent for research applications. The compound is intended for research use only.
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| Enzyme Assay |
In vitro labeling assays for 6-ROX hydrochloride involve incubating the carboxylic acid with amine-containing biomolecules in the presence of carbodiimide coupling reagents. The reaction forms stable amide bonds between the carboxyl and amine groups. Labeling efficiency can be assessed by measuring the fluorescence intensity of the conjugated product.
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| Cell Assay |
For in vitro cellular experiments, 6-ROX hydrochloride is conjugated to oligonucleotides, antibodies, or other targeting ligands via the carboxylic acid group. The labeled conjugates are then added to cell culture medium for incubation with target cells. After washing, cells are analyzed by fluorescence microscopy or flow cytometry. The red fluorescence provides bright and photostable labeling.
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| Animal Protocol |
In vivo animal experiments with 6-ROX hydrochloride are not typically performed as a therapeutic intervention. However, the dye can be used for in vivo imaging applications when conjugated to targeting ligands. The red fluorescence provides good tissue penetration and low autofluorescence background. Conjugates bearing 6-ROX can be administered to animal models for tracking biodistribution and target engagement.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 6-ROX hydrochloride have not been characterized, as the compound is a research-use fluorescent labeling reagent rather than a drug candidate. The compound has a molecular weight of 571.06 g/mol and a molecular formula of C₃₃H₃₁ClN₂O₅. It should be stored at 4°C, away from moisture.
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| Toxicity/Toxicokinetics |
Toxicological data for 6-ROX hydrochloride has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent. The compound is moisture-sensitive and should be protected from moisture.
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| Additional Infomation |
6-ROX hydrochloride (6-Carboxy-X-rhodamine hydrochloride) is the single-isomer hydrochloride salt of 6-carboxy-X-rhodamine, a red-emitting fluorescent label. The compound is used as a fluorescent label for oligonucleotides and works as an acceptor coupled to 5-FAM and as a donor in FRET imaging. The compound has no clinical or therapeutic applications and has not received regulatory approval.
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| Molecular Formula |
C33H31CLN2O5
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|---|---|
| Molecular Weight |
571.062648057938
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| Exact Mass |
570.192
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| CAS # |
1689512-96-6
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| Related CAS # |
6-ROX;194785-18-7
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| PubChem CID |
166642655
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| Appearance |
Brown to black solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
41
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| Complexity |
1200
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(C1C=CC(C(=O)O)=CC=1C1=C2C=C3CCCN4CCCC(=C34)C2=[O+]C2=C3CCCN4CCCC(=C34)C=C12)(=O)O.[Cl-]
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| InChi Key |
HYKDMQKZQYLGAN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C33H30N2O5.ClH/c36-32(37)20-9-10-21(33(38)39)24(17-20)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31;/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39);1H
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| Chemical Name |
4-carboxy-3-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoate;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7511 mL | 8.7556 mL | 17.5113 mL | |
| 5 mM | 0.3502 mL | 1.7511 mL | 3.5023 mL | |
| 10 mM | 0.1751 mL | 0.8756 mL | 1.7511 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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