| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Biochemical Assay Reagent
|
|---|---|
| ln Vitro |
3,3-Diaminobenzidine tetrahydrochloride dehydrate (DAB) is a staining agent that, upon oxidation, forms an insoluble polymer and deposits as a dark brown precipitate at the reaction site, thereby visualizing target molecules. In immunohistochemical applications, this compound serves as a substrate for peroxidase conjugated to primary or secondary antibodies. Its histochemical staining technique is primarily used in the following areas: organelle staining of peroxisomes and activity detection of mitochondrial cytochrome c oxidase (COX, also known as respiratory complex IV) [1].
Guidelines [1] (The following is a recommended protocol and can be adjusted according to specific needs): Cytochrome c oxidase (COX) histochemical staining procedure: 1. Incubate the sections with approximately 50 µL of COX staining solution (containing 0.1 M NaPO₄ buffer, 4 mM 3,3-diaminobenzidine tetrahydrochloride dehydrate, 100 µM cytochrome c, and 2 mg/mL catalase) at 37°C for 45 minutes. 2. Rinse three times with PBS buffer, 5 minutes each, followed by graded ethanol dehydration (75% and 95% ethanol for 1 minute each, and 100% ethanol for 10 minutes). 3. After staining, allow the sample to dry completely before further processing. |
| References |
[1]. Dölle C, et al. 3,3'-Diaminobenzidine staining interferes with PCR-based DNA analysis. Sci Rep. 2018 Jan 19;8(1):1272.
|
| Molecular Formula |
C12H22CL4N4O2
|
|---|---|
| Molecular Weight |
214.2664
|
| Exact Mass |
394.049
|
| CAS # |
167684-17-5
|
| Related CAS # |
DAB tetrahydrochloride;7411-49-6
|
| PubChem CID |
2829715
|
| Appearance |
Typically exists as solid at room temperature
|
| Boiling Point |
481.7ºC at 760 mmHg
|
| Melting Point |
280 °C
|
| Flash Point |
282.7ºC
|
| Vapour Pressure |
1.95E-09mmHg at 25°C
|
| LogP |
7.086
|
| Hydrogen Bond Donor Count |
10
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
22
|
| Complexity |
209
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
N([H])([H])C1=C(C([H])=C([H])C(=C1[H])C1C([H])=C([H])C(=C(C=1[H])N([H])[H])N([H])[H])N([H])[H]
|
| InChi Key |
BXIYIGBKSGVJOS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C12H14N4.4ClH.2H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;;/h1-6H,13-16H2;4*1H;2*1H2
|
| Chemical Name |
4-(3,4-diaminophenyl)benzene-1,2-diamine;dihydrate;tetrahydrochloride
|
| Synonyms |
167684-17-5; 3,3'-Diaminobenzidine tetrahydrochloride dihydrate; (1,1'-Biphenyl)-3,3',4,4'-tetramine tetrahydrochloride dihydrate; UNII-Z3KH58591V; Z3KH58591V; [1,1'-Biphenyl]-3,3',4,4'-tetramine, hydrochloride, hydrate (1:4:2); (1,1'-Biphenyl)-3,3',4,4'-tetramine, tetrahydrochloride, dihydrate; (1,1'-Biphenyl)-3,3',4,4'-tetramine, hydrochloride, hydrate (1:4:2);
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6670 mL | 23.3350 mL | 46.6701 mL | |
| 5 mM | 0.9334 mL | 4.6670 mL | 9.3340 mL | |
| 10 mM | 0.4667 mL | 2.3335 mL | 4.6670 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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