| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
Lipid droplets and mitochondria.
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|---|---|
| ln Vitro |
Not applicable as BETA-1 is a dual-color organelle imaging probe.
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| Enzyme Assay |
In cultured cells (e.g., HeLa, 3T3-L1 adipocytes, or primary hepatocytes), BETA-1 at working concentrations (0.5-5 uM) rapidly stains lipid droplets (cyan emission) and mitochondria (red emission) with high selectivity and minimal cross-talk. The probe can be used to study lipid metabolism, mitochondrial dynamics (fusion/fission), and organelle interactions (e.g., LD-mitochondria contacts) under various physiological and pathological conditions (e.g., steatosis, oxidative stress).
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| Cell Assay |
Standard cell staining protocol: Prepare a 1 mM stock solution of BETA-1 in DMSO. Dilute to working solution (1-5 uM) in pre-warmed cell culture medium (serum-free or complete). Remove culture medium from adherent cells grown on coverslips or glass-bottom dishes. Add BETA-1 working solution and incubate at 37degC for 15-30 minutes in the dark. Wash cells twice with PBS to remove excess dye. Add fresh medium or PBS. For live-cell imaging, use a confocal laser scanning microscope: LD channel: Ex/Em ~400-450 nm / 460-500 nm; Mitochondria channel: Ex/Em ~550-580 nm / 600-650 nm.
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| Animal Protocol |
Dissolve BETA-1 in DMSO, then dilute in a biocompatible vehicle (e.g., 10% DMSO, 40% PEG300, 5% Tween-80, 45% saline). Administer to zebrafish larvae (immersed in embryo medium, 1-10 uM for 1-2 hours) or mice via tail vein injection (2-5 mg/kg). For mice, allow circulation for 1-2 hours, then anesthetize and perform intravital imaging of tissues (liver, adipose tissue, skeletal muscle). Euthanize animals, harvest organs, and image ex vivo. BETA-1 exhibits good tissue penetration and minimal background due to its AIE property.
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| ADME/Pharmacokinetics |
BETA-1 has a molecular weight of 705.67 g/mol (containing iodine). The TICT-AIE integration provides excellent photostability and resistance to photobleaching. In vivo, BETA-1 accumulates predominantly in lipid-rich tissues (liver, adipose) and metabolically active tissues with high mitochondrial content (heart, skeletal muscle). The probe is slowly cleared from the body (t1/2 >12 hours) due to its lipophilic nature and iodine atoms, which may contribute to retention in adipose stores.
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| Toxicity/Toxicokinetics |
Preliminary cytotoxicity assays in cell lines show low toxicity at working concentrations (1-5 uM, 24 hours). In zebrafish larvae, no developmental toxicity or mortality is observed at concentrations up to 20 uM. For mice, single-dose acute toxicity at imaging doses (2-5 mg/kg IV) has not reported adverse effects. No chronic toxicity data is available.
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| References | |
| Additional Infomation |
BETA-1 is a first-in-class research tool for studying the dynamic interplay between lipid droplets and mitochondria, two key organelles involved in energy metabolism, lipotoxicity, and cell death pathways. It has not been clinically approved. The probe's unique dual-color emission eliminates the need for multiple probes or complex spectral unmixing, making it highly advantageous for high-throughput screening of metabolic modulators.
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| CAS # |
2924598-24-1
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|---|---|
| Appearance |
Brown to black solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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