| Size | Price | Stock | Qty |
|---|---|---|---|
| 25mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| 1g | |||
| Other Sizes |
| Targets |
As a fluorescent probe, NBD-X acid does not target specific proteins or enzymes. Instead, it is used for studying fatty acids and sterols, as well as for labeling biopolymers. The carboxylic acid group provides a reactive site for conjugation to amines through carbodiimide-mediated coupling reactions.
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|---|---|
| ln Vitro |
The in vitro activity of NBD-X acid is characterized by its environment-sensitive fluorescence. NBD-X acid is a fluorescent probe for the study of fatty acids and sterols. Compared to NBD chloride and fluoride, NBD-X acid provides better yields for labeling biopolymers. The NBD fluorophore exhibits green to yellow fluorescence, with excitation/absorption wavelengths of 465/535 nm. The fluorescence intensity is sensitive to the local environment, making it useful for studying binding sites and membrane interactions.
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| ln Vivo |
No significant in vivo activity data has been reported for NBD-X acid as a therapeutic agent, as the compound is primarily utilized as a research reagent for labeling and fluorescence studies. The compound is intended for research use only.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for NBD-X acid as it is a fluorescent labeling reagent rather than a drug targeting specific enzymes or receptors. The compound can be used to label biomolecules through the carboxylic acid group using carbodiimide-mediated coupling reactions. The labeled conjugates can then be used in binding studies to investigate interactions with fatty acid and sterol carrier proteins.
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| Cell Assay |
For in vitro cellular experiments, NBD-X acid is dissolved in DMSO and diluted in cell culture medium. The compound can be conjugated to biomolecules of interest via the carboxylic acid group. After labeling, cells are incubated with the NBD-X acid-labeled probes and analyzed by fluorescence microscopy or flow cytometry. The NBD fluorophore's environment-sensitive fluorescence makes it useful for studying membrane dynamics and protein-lipid interactions.
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| Animal Protocol |
In vivo animal experiments with NBD-X acid are not typically performed, as the compound is primarily used as a research reagent for in vitro labeling and fluorescence studies. The compound is intended for research use only and not for in vivo therapeutic applications.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of NBD-X acid have not been characterized, as the compound is a research-use fluorescent probe rather than a drug candidate. The compound has a molecular weight of 294.27 g/mol and is soluble in DMSO. It should be stored frozen at < -15°C and protected from light.
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| Toxicity/Toxicokinetics |
Toxicological data for NBD-X acid indicates the compound is classified with hazard symbols XN and hazard phrases H303, H313, H333. The compound is intended for research use only and not for human therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
7-Nitrobenzoxadiazole-6-aminohexanoic acid is a benzoxadiazole formed by substituting (5-carboxypentyl)amino and nitro groups at the 4 and 7 positions of 2,1,3-benzoxadiazole, respectively. It can be used as a fluorescent dye. It is a benzoxadiazole, C-nitro compound, secondary amino compound, and ε-amino acid.
NBD-X acid (C6-NBD) is a fluorescent probe based on the environmentally sensitive NBD fluorophore, featuring a hexanoic acid tail for conjugation. It is used for studying fatty acids and sterols, as well as for labeling biopolymers. The NBD fluorophore's environment-sensitive fluorescence makes it valuable for studying binding sites of fatty acid and sterol carrier proteins and for cell membrane staining. The compound has no clinical or therapeutic applications and has not received regulatory approval. |
| Molecular Formula |
C12H14N4O5
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|---|---|
| Molecular Weight |
294.263362407684
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| Exact Mass |
294.096
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| CAS # |
88235-25-0
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| PubChem CID |
139072
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.462
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| Boiling Point |
559.4ºC at 760 mmHg
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| Index of Refraction |
1.65
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| LogP |
2.784
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
21
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| Complexity |
375
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C(CCCCCNC1C2C(=NON=2)C([N+](=O)[O-])=CC=1)O
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| InChi Key |
DJFNQJJTTPMBIL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H14N4O5/c17-10(18)4-2-1-3-7-13-8-5-6-9(16(19)20)12-11(8)14-21-15-12/h5-6,13H,1-4,7H2,(H,17,18)
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| Chemical Name |
6-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]hexanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3984 mL | 16.9918 mL | 33.9836 mL | |
| 5 mM | 0.6797 mL | 3.3984 mL | 6.7967 mL | |
| 10 mM | 0.3398 mL | 1.6992 mL | 3.3984 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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