| Size | Price | Stock | Qty |
|---|---|---|---|
| 500μg |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Click chemistry reagent
As a click chemistry reagent containing an azide group, Sulfo-Cy5-N3 does not target specific proteins or enzymes. Instead, the azide functional group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) or strain-promoted azide-alkyne cycloaddition (SPAAC) reactions with alkyne-bearing biomolecules. This allows the covalent attachment of the Cy5 fluorophore to target biomolecules for subsequent fluorescence detection and imaging applications. |
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| ln Vitro |
Nanoparticles are excellent imaging agents for cancer, but variability in chemical structure, racemic mixtures, and addition of heavy metals hinders FDA approval in the United States. We developed a small ultra-red fluorescent protein, named smURFP, to have optical properties similar to the small-molecule Cy5, a heptamethine subclass of cyanine dyes (Ex/Em = 642/670 nm). smURFP has a fluorescence quantum yield of 18% and expresses so well in E. coli, that gram quantities of fluorescent protein are purified from cultures in the laboratory. In this research, the fluorescent protein smURFP was combined with bovine serum albumin into fluorescent protein nanoparticles. These nanoparticles are fluorescent with a quantum yield of 17% and 12-14 nm in diameter. The far-red fluorescent protein nanoparticles noninvasively image tumors in living mice via the enhanced permeation and retention (EPR) mechanism. This manuscript describes the use of a new fluorescent protein nanoparticle for in vivo fluorescent imaging. This protein nanoparticle core should prove useful as a biomacromolecular scaffold, which could bear extended chemical modifications for studies, such as the in vivo imaging of fluorescent protein nanoparticles targeted to primary and metastatic cancer, theranostic treatment, and/or dual-modality imaging with positron emission tomography for entire human imaging [1].
The in vitro activity of Sulfo-Cy5-N3 is characterized by its strong fluorescence properties in the far-red to near-infrared spectrum, which provides deep tissue penetration and low background fluorescence. The dye exhibits high photostability and bright fluorescence suitable for multiplexing in imaging and flow cytometry experiments. The compound's water solubility makes it compatible with aqueous biological systems, enabling efficient labeling of biomolecules in physiological conditions. |
| ln Vivo |
No significant in vivo activity data has been reported for Sulfo-Cy5-N3 as a therapeutic agent, as the compound is primarily utilized as a fluorescent labeling reagent for research applications. The compound is intended for research use only and not for diagnostic or medical purposes.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for Sulfo-Cy5-N3 as it is a fluorescent labeling reagent rather than a drug targeting specific enzymes or receptors. The compound is used in click chemistry reactions where it is incubated with alkyne-modified biomolecules under appropriate reaction conditions (CuAAC or SPAAC). The efficiency of labeling can be assessed by measuring the fluorescence intensity of the conjugated product using fluorescence spectroscopy, flow cytometry, or fluorescence microscopy.
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| Cell Assay |
Cell imaging with the fluorescent protein nanoparticles A549 cancer cells were seeded in 24-well plate and cultured for 24 h at 37 °C. The cells were incubated with 1 μM fluorescent protein nanoparticles and after 24 h, the cells were washed three times with 1x PBS, pH 7.4 and stained with DAPI. The cells were imaged with EVOS fluorescence microscope with a Cy5 LED cube (Ex/Em: 628(40)/692(40) nm) [1].
For in vitro cellular experiments, Sulfo-Cy5-N3 is dissolved in DMSO or water and diluted in cell culture medium. Cells or biomolecules of interest are first modified with alkyne functional groups, then incubated with Sulfo-Cy5-N3 under click chemistry reaction conditions. After washing to remove unreacted dye, the labeled cells or biomolecules are analyzed by fluorescence microscopy, flow cytometry, or fluorescence spectroscopy. The strong far-red fluorescence enables multiplexing with other fluorophores and provides low background autofluorescence. |
| Animal Protocol |
Noninvasive fluorescence imaging of A549 tumor xenograft in vivo
In vivo animal experiments with Sulfo-Cy5-N3 are not typically performed as a therapeutic intervention. However, the compound can be used for in vivo imaging applications when conjugated to targeting ligands or biomolecules via click chemistry. The near-infrared emission of Cy5 provides deep tissue penetration and low background fluorescence, making it suitable for in vivo imaging studies. Conjugates bearing Sulfo-Cy5-N3 can be administered to animal models for tracking biodistribution and target engagement. |
| ADME/Pharmacokinetics |
Pharmacokinetic properties of Sulfo-Cy5-N3 have not been characterized, as the compound is a research-use fluorescent labeling reagent rather than a drug candidate. The compound has a molecular weight of 724.89 g/mol and is water-soluble due to the sulfonate groups. The compound should be stored at -20°C and protected from light to maintain stability.
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| Toxicity/Toxicokinetics |
Toxicological data for Sulfo-Cy5-N3 has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent. The compound is light-sensitive and should be protected from light during storage and handling.
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| References | |
| Additional Infomation |
Sulfo-Cy5-N3 (disulfo-Cy5-azide) is a water-soluble, sulfonated Cy5 fluorescent dye bearing an azide group for bioorthogonal click chemistry labeling. The compound is particularly valuable in the detection, quantification, and visualization of biomolecules in live cell assays, tissue analysis, and high-throughput screening. Cy5 is a popular red fluorescent label dye compatible with different fluorescence measuring instruments. The compound has no clinical or therapeutic applications and has not received regulatory approval.
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| Molecular Formula |
C35H44N6O7S2
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|---|---|
| Exact Mass |
746.253
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| CAS # |
1481447-40-8
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| PubChem CID |
71777733
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| Appearance |
Brown to dark brown solid powder
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
13
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| Heavy Atom Count |
51
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| Complexity |
1610
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1/C=C/C=C/C=C/3\C(C4=C(N3CCCCCC(=O)NCCCN=[N+]=[N-])C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C.[Na+]
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| InChi Key |
OCCGJERHFSRPEN-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C35H44N6O7S2.Na/c1-34(2)27-23-25(49(43,44)45)16-18-29(27)40(5)31(34)13-8-6-9-14-32-35(3,4)28-24-26(50(46,47)48)17-19-30(28)41(32)22-11-7-10-15-33(42)37-20-12-21-38-39-36;/h6,8-9,13-14,16-19,23-24H,7,10-12,15,20-22H2,1-5H3,(H2-,37,42,43,44,45,46,47,48);/q;+1/p-1
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| Chemical Name |
sodium;1-[6-(3-azidopropylamino)-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
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| Synonyms |
Sodium;1-[6-(3-azidopropylamino)-6-oxohexyl]-3,3-dimethyl-2-[5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 125 mg/mL (172.44 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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