| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Msr-Ratio targets methionine sulfoxide reductase (Msr) enzymes, which catalyze the reduction of methionine sulfoxide to methionine. The compound serves as a substrate for Msr enzymes, with enzymatic activity resulting in a change in fluorescence properties.
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|---|---|
| ln Vitro |
Methionine sulfoxide reductase transforms Msr-Ratio into its matching sulfide form. Positive characteristics of Msr-Ratio include a nearly 400-fold change in fluorescence, a quick response time (less than 30 minutes), a significant Stokes shift (120 nm), and green emission (550 nm)[1]. Following the addition of Msr-Ratio, live HL60 cells exhibit an extremely weak background signal. The fluorescence signal rose steadily from 0 to 8 hours as the Msr-Ratio (10 μM) incubation duration in cells increased[1].
The in vitro activity of Msr-Ratio is its function as a ratiometric fluorescent probe for detecting Msr activity. The compound undergoes a change in fluorescence properties upon enzymatic reduction by Msr, enabling the quantification of Msr activity through ratiometric fluorescence measurements. |
| ln Vivo |
Msr-Ratio is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the detection and imaging of Msr activity in living cells and tissues. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro assays for Msr-Ratio involve incubating the probe with Msr enzyme preparations in appropriate buffer conditions. The enzymatic reduction of the probe results in a change in fluorescence properties, which can be measured ratiometrically to quantify Msr activity.
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| Cell Assay |
For in vitro cellular experiments, Msr-Ratio is used to detect and image Msr activity in living cells. Cells are incubated with the probe, and the ratiometric fluorescence signal is measured using fluorescence microscopy or spectroscopy to quantify Msr activity in the cellular environment.
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| Animal Protocol |
In vivo animal experiments with Msr-Ratio are not typically performed, as the compound is primarily used as a research tool for detecting Msr activity in cells. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Msr-Ratio have not been characterized, as the compound is a research-use fluorescent probe rather than a drug candidate. The compound should be stored under appropriate conditions, protected from light.
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| Toxicity/Toxicokinetics |
Toxicological data for Msr-Ratio has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
Msr-Ratio is a ratiometric fluorescent probe for detecting and imaging methionine sulfoxide reductase activity in living cells. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C18H14O3S
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|---|---|
| Molecular Weight |
310.37
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| Exact Mass |
310.066
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| CAS # |
2290635-22-0
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| PubChem CID |
162642076
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| Appearance |
White to off-white solid powder
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| LogP |
3.3
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
501
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C2C(C(=O)C=C(/C=C/C3C=CC(S(C)=O)=CC=3)O2)=C1
|
| InChi Key |
ICJBRZQQFOCOJU-RMKNXTFCSA-N
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| InChi Code |
InChI=1S/C18H14O3S/c1-22(20)15-10-7-13(8-11-15)6-9-14-12-17(19)16-4-2-3-5-18(16)21-14/h2-12H,1H3/b9-6+
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| Chemical Name |
2-[(E)-2-(4-methylsulfinylphenyl)ethenyl]chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2220 mL | 16.1098 mL | 32.2196 mL | |
| 5 mM | 0.6444 mL | 3.2220 mL | 6.4439 mL | |
| 10 mM | 0.3222 mL | 1.6110 mL | 3.2220 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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