| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| Other Sizes |
| Targets |
HIDC does not target a specific protein or enzyme. As a cyanine dye, its utility in research is based on its photophysical properties, particularly its NIR fluorescence and ability to intercalate into lipid membranes.
|
|---|---|
| ln Vitro |
The in vitro activity of HIDC is characterized by its strong near-infrared fluorescence, with excitation and emission maxima around 740 nm and 760 nm, respectively. The NIR emission provides deep tissue penetration and low background fluorescence, making it suitable for imaging applications.
|
| ln Vivo |
HIDC is not used as a therapeutic agent. Its in vivo utility is as a NIR fluorescent probe for imaging applications. The NIR emission provides deep tissue penetration and low background fluorescence, making it suitable for in vivo imaging studies.
|
| Enzyme Assay |
In vitro binding assays are not applicable for HIDC as it functions as a fluorescent dye rather than a drug targeting specific enzymes or receptors. The compound's interaction with biological samples is based on its photophysical properties and ability to intercalate into membranes.
|
| Cell Assay |
For in vitro cellular experiments, HIDC is dissolved in DMSO and diluted in cell culture medium. Cells are incubated with the dye and analyzed by fluorescence microscopy or flow cytometry. The NIR fluorescence provides deep tissue penetration and low background for imaging applications.
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| Animal Protocol |
For in vivo animal experiments, HIDC can be used as a NIR fluorescent probe for imaging studies. The dye can be administered to animal models for tracking biodistribution and imaging applications.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of HIDC have not been extensively characterized. The compound has a molecular weight of 665.42 g/mol and should be stored under appropriate conditions, protected from light.
|
| Toxicity/Toxicokinetics |
Toxicological data for HIDC has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed.
|
| Additional Infomation |
HIDC is a near-infrared cyanine dye used as a fluorescent probe for imaging applications, exhibiting strong absorption and fluorescence in the NIR region. The compound has no clinical or therapeutic applications.
|
| Molecular Formula |
C27H31IN2
|
|---|---|
| Molecular Weight |
510.45
|
| Exact Mass |
510.153
|
| CAS # |
36536-22-8
|
| Related CAS # |
48221-03-0 (Parent)
|
| PubChem CID |
5702727
|
| Appearance |
Green to dark green solid powder
|
| Melting Point |
264ºC (dec.)(lit.)
|
| LogP |
3.144
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
30
|
| Complexity |
689
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1(C2=CC=CC=C2[N+](=C1/C=C/C=C/C=C/3\C(C4=CC=CC=C4N3C)(C)C)C)C.[I-]
|
| InChi Key |
UANMYOBKUNUUTR-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C27H31N2.HI/c1-26(2)20-14-10-12-16-22(20)28(5)24(26)18-8-7-9-19-25-27(3,4)21-15-11-13-17-23(21)29(25)6;/h7-19H,1-6H3;1H/q+1;/p-1
|
| Chemical Name |
(2E)-1,3,3-trimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indole;iodide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9591 mL | 9.7953 mL | 19.5906 mL | |
| 5 mM | 0.3918 mL | 1.9591 mL | 3.9181 mL | |
| 10 mM | 0.1959 mL | 0.9795 mL | 1.9591 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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