| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Azomethine-H monosodium does not target a specific protein or enzyme. Its utility as a chromogenic reagent is based on its chemical reaction with borate ions to form a colored complex that can be detected spectrophotometrically.
|
|---|---|
| ln Vitro |
For the manual determination of boron in complex and boranes, Azomethine-H monosodium is utilized[2].
The in vitro activity of Azomethine-H monosodium is its function as a chromogenic reagent for the determination of boron. The compound reacts with borate ions in solution to form a yellow-colored complex with maximum absorbance at 420 nm. The intensity of the color is proportional to the boron concentration, enabling quantitative analysis. |
| ln Vivo |
Azomethine-H monosodium is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the determination of boron content in biological samples. The compound is not intended for systemic therapeutic use.
|
| Enzyme Assay |
In vitro assays for Azomethine-H monosodium involve incubating the reagent with samples containing borate ions in appropriate buffer conditions. The reaction forms a yellow-colored complex that can be quantified by measuring absorbance at 420 nm. The absorbance is proportional to the boron concentration in the sample.
|
| Cell Assay |
For in vitro cellular experiments, Azomethine-H monosodium is not typically used in cell-based assays. The compound is used as a chemical reagent for the determination of boron content in various sample types, including biological fluids and tissues.
|
| Animal Protocol |
In vivo animal experiments with Azomethine-H monosodium are not typically performed, as the compound is used as an in vitro analytical reagent. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Azomethine-H monosodium have not been characterized, as the compound is a research-use analytical reagent rather than a drug candidate. The compound has a molecular weight of 445.40 g/mol and should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for Azomethine-H monosodium has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed.
|
| References | |
| Additional Infomation |
Azomethine-H monosodium is a chromogenic reagent used for the spectrophotometric determination of boron in soil, water, plants, and biological samples, reacting with borate ions to form a yellow-colored complex. The compound has no clinical or therapeutic applications.
|
| Molecular Formula |
C17H12NNAO8S2
|
|---|---|
| Molecular Weight |
445.40
|
| Exact Mass |
463
|
| CAS # |
5941-07-1
|
| Related CAS # |
5941-07-1 (mono-hydrochloride salt)
|
| PubChem CID |
94428
|
| Appearance |
Yellow to orange solid powder
|
| Density |
1.823g/cm3
|
| Melting Point |
>300°C
|
| Index of Refraction |
1.81
|
| LogP |
4.314
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
28
|
| Complexity |
785
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C(C(=C1)C=NC2=C3C(=CC(=C2)S(=O)(=O)[O-])C=C(C=C3O)S(=O)(=O)O)O.O.[Na+]
|
| InChi Key |
DUCCKQSNXPFEGT-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C17H13NO8S2/c19-15-4-2-1-3-10(15)9-18-14-7-12(27(21,22)23)5-11-6-13(28(24,25)26)8-16(20)17(11)14/h1-9,19-20H,(H,21,22,23)(H,24,25,26)
|
| Chemical Name |
4-hydroxy-5-[(2-hydroxyphenyl)methylideneamino]naphthalene-2,7-disulfonic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 83.33 mg/mL (187.09 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2452 mL | 11.2259 mL | 22.4517 mL | |
| 5 mM | 0.4490 mL | 2.2452 mL | 4.4903 mL | |
| 10 mM | 0.2245 mL | 1.1226 mL | 2.2452 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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