| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
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| 5mg | |||
| Other Sizes |
| Targets |
Thiols (Biothiols: glutathione (GSH), cysteine (Cys), homocysteine (Hcy)).
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|---|---|
| ln Vitro |
In cell-free systems, BODIPY-TS demonstrates fast response kinetics (within 2-5 min) upon reaction with thiols. The fluorescence intensity increases linearly with thiol concentration, with a low detection limit reported for biothiols (e.g., low nM range). It exhibits excellent selectivity for biothiols over other biologically relevant species, including amino acids (without thiols), reactive oxygen species, and metal ions.
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| Enzyme Assay |
A typical assay: Prepare a 10 uM BODIPY-TS solution in 100 mM PBS (pH 7.4). Add various concentrations of thiols (e.g., GSH, Cys, Hcy: 0-100 uM) and incubate at 25degC for 2-10 min. Measure fluorescence intensity using a plate reader or fluorometer with Ex/Em at 490 nm/515 nm. Determine selectivity by testing with non-thiol amino acids (e.g., alanine, serine), ROS (H2O2), and metal ions (e.g., Zn2+, Cu2+) under the same conditions.
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| Cell Assay |
Standard cell imaging protocol: Plate cells (e.g., HeLa or RAW 264.7) onto coverslips or 96-well plates. After overnight culture, treat cells with N-ethylmaleimide (NEM, 1 mM) for 30 min to block endogenous thiols (negative control). Wash cells with PBS. Incubate cells with 5-20 uM BODIPY-TS in serum-free medium for 30 min at 37degC. Wash cells three times with PBS to remove unbound dye. Mount coverslips or add fresh medium to wells. Image cells using a fluorescence microscope with FITC filter (Ex~490 nm, Em~515 nm).
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| Animal Protocol |
Formulate BODIPY-TS for animal experiments: Dissolve in DMSO (stock), then dilute with saline or PBS to working concentration (final DMSO <5%). Administer to mice via intraperitoneal (IP) injection at 10-20 mg/kg. Allow circulation for 30-60 min. Euthanize animals, harvest organs (liver, kidney, tumor), prepare homogenates, and measure fluorescence. For in vivo imaging in zebrafish or mice, anesthetize animals, inject probe intravenously or locally, and perform fluorescence imaging using an IVIS system or confocal microscope with GFP filter settings.
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| ADME/Pharmacokinetics |
BODIPY-TS has a molecular weight of 434.33 g/mol. It is highly soluble in DMSO and moderately soluble in aqueous buffers. In animal models, following intravenous administration, BODIPY-TS distributes rapidly to highly perfused organs such as the liver and kidneys. The probe undergoes a rapid thiol-cleavage reaction, and the fluorescent product accumulates in these tissues. The half-life (t1/2) in circulation is estimated to be 1-2 hours, with metabolism primarily mediated by hepatic glutathione conjugation.
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| Toxicity/Toxicokinetics |
Cytotoxicity assessments using MTT assays in HeLa and HEK293 cell lines indicate that BODIPY-TS exhibits low toxicity at concentrations up to 50 uM for 24 hours (cell viability >90%). In animal studies at standard imaging doses (10-20 mg/kg, single administration), no gross signs of toxicity (e.g., weight loss, behavioral changes, or mortality) have been reported.
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| References | |
| Additional Infomation |
BODIPY-TS is a research-grade reagent primarily used for detecting thiol levels in biological systems, with applications in redox biology, oxidative stress, and anticancer drug screening. It has not been evaluated in clinical trials nor approved for diagnostic or therapeutic use in humans.
|
| Molecular Formula |
C20H21BN2O2F2S2
|
|---|---|
| Molecular Weight |
434.331
|
| Exact Mass |
434.11
|
| CAS # |
1808174-52-8
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| PubChem CID |
162642081
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| Appearance |
Orange to red solid powder
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| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
|
| Heavy Atom Count |
29
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| Complexity |
873
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1C=C(C)[N-]2[B+3]([F-])([F-])N3=C(C)C=C(C)C3=C(C3C=CC(SS(=O)(=O)C)=CC=3)C=12
|
| InChi Key |
RWWARNPLGWXOFT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H21BF2N2O2S2/c1-12-10-14(3)24-19(12)18(16-6-8-17(9-7-16)28-29(5,26)27)20-13(2)11-15(4)25(20)21(24,22)23/h6-11H,1-5H3
|
| Chemical Name |
2,2-difluoro-4,6,10,12-tetramethyl-8-(4-methylsulfonylsulfanylphenyl)-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (230.24 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3024 mL | 11.5120 mL | 23.0240 mL | |
| 5 mM | 0.4605 mL | 2.3024 mL | 4.6048 mL | |
| 10 mM | 0.2302 mL | 1.1512 mL | 2.3024 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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