| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Mitochondrial Mg2+ ions (Mg2+) are the primary molecular target. The probe is a prodrug that is designed to accumulate in mitochondria and chelate Mg2+ in this organelle.
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|---|---|
| ln Vitro |
KMG-301 is a Mg2+-selective fluorescent probe that functions in mitochondria of intact cells. Since KMG-301 does not penetrate the mitochondrial membrane, mitochondrial membrane potential depolarization does not release KMG-301. KMG-301 can indicate mitochondrial Mg2+ concentration.
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| ln Vivo |
There is no specific in vivo activity data available for this compound. As a Mg2+-selective fluorescent probe, it can be used in live cells and isolated mitochondria to study Mg2+ dynamics. A typical protocol involves using it to acquire time-course and pseudo-colored images of Mg2+ changes.
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| Enzyme Assay |
A standard protocol involves incubating KMG-301AM (e.g., 5-10 uM) with isolated mitochondria in an appropriate buffer for 30-60 minutes at 37degC to allow for hydrolysis to KMG-301. The fluorescence of KMG-301 (Ex/Em: typically around 500/535 nm) is then measured upon addition of Mg2+ ions.
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| Cell Assay |
A general protocol for cellular imaging involves seeding cells in a confocal dish and incubating with KMG-301AM (e.g., 5-10 uM) for 30-60 minutes at 37degC. After washing, the cells are imaged using a confocal microscope to visualize mitochondrial Mg2+ levels. A Mg2+ ionophore (e.g., ionomycin) can be used to modulate Mg2+ levels as a control.
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| Animal Protocol |
A typical protocol for in vivo imaging involves intravenous or intraperitoneal injection of an optimized concentration of KMG-301AM into a live animal model. After a suitable circulation time (e.g., 30 minutes to 2 hours), the animal is placed under an in vivo imaging system to monitor Mg2+-induced fluorescence.
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| ADME/Pharmacokinetics |
General PK properties for AM ester probes: The AM ester group enhances cell permeability and is cleaved by intracellular esterases to trap the probe inside cells, particularly in mitochondria. Free KMG-301 is membrane-impermeable and accumulates in mitochondria.
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| Toxicity/Toxicokinetics |
General toxicity for imaging probes is considered low. For KMG-301AM, no specific toxicity studies are reported. In similar experiments, incubation with up to 10 uM for 24 hours in cell models shows good biocompatibility and no significant cytotoxicity.
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| References | |
| Additional Infomation |
KMG-301AM is a research tool for studying magnesium ion homeostasis, particularly within mitochondria. It enables the selective detection of Mg2+ dynamics in this organelle and can be used to investigate the role of Mg2+ in mitochondrial function, apoptosis, and metabolic regulation. This product is for research use only.
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| Molecular Formula |
C30H28N3O6
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|---|---|
| Molecular Weight |
526.56
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| Exact Mass |
526.198
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| CAS # |
1372642-77-7
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| PubChem CID |
138911329
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| Appearance |
Brown to red liquid
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| Hydrogen Bond Donor Count |
0
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
39
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| Complexity |
1320
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)OCOC(=O)C1=CC(=C2C=CC=CN2C1=O)C3=C4C=CC(=[N+](C)C)C=C4OC5=C3C=CC(=C5)N(C)C
|
| InChi Key |
JNVNRNBXFHGZHT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C30H28N3O6/c1-18(34)37-17-38-30(36)24-16-23(25-8-6-7-13-33(25)29(24)35)28-21-11-9-19(31(2)3)14-26(21)39-27-15-20(32(4)5)10-12-22(27)28/h6-16H,17H2,1-5H3/q+1
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| Chemical Name |
[9-[3-(acetyloxymethoxycarbonyl)-4-oxoquinolizin-1-yl]-6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8991 mL | 9.4956 mL | 18.9912 mL | |
| 5 mM | 0.3798 mL | 1.8991 mL | 3.7982 mL | |
| 10 mM | 0.1899 mL | 0.9496 mL | 1.8991 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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