| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| Other Sizes |
| Targets |
Azure A chloride does not target a specific protein or enzyme. As a dye, its utility in staining and electrophoresis is based on its chemical properties, particularly its ability to bind to nucleic acids and other cellular components.
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|---|---|
| ln Vitro |
The in vitro activity of Azure A chloride is its function as a DNA dye and histological stain. The compound binds to DNA and can be used to visualize nucleic acids in agarose gel electrophoresis and PAGE. It is also used in azure eosin stains for blood smear staining. Azure A chloride can be used for the study of stains and redox media for electrochemical biosensing.
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| ln Vivo |
Azure A chloride is not used as a therapeutic agent. Its in vivo utility is limited to research and diagnostic applications as a stain. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro binding assays are not applicable for Azure A chloride as it functions as a DNA dye and stain rather than a drug targeting specific enzymes or receptors. The compound is used in staining protocols where it binds to nucleic acids and cellular components.
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| Cell Assay |
For in vitro cellular experiments, Azure A chloride is used in staining procedures for blood smears and other biological samples. The compound provides contrast for the visualization of cellular structures under a microscope.
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| Animal Protocol |
In vivo animal experiments with Azure A chloride are not typically performed, as the compound is primarily used as an in vitro stain. The compound is intended for research and diagnostic use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Azure A chloride have not been characterized, as the compound is a research-use dye rather than a drug candidate. The compound has a molecular weight of 291.80 g/mol and should be stored under appropriate conditions.
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| Toxicity/Toxicokinetics |
Toxicological data for Azure A chloride has not been systematically evaluated, as the compound is intended for research and diagnostic applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References |
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| Additional Infomation |
Azure A is an organochloride salt with a counterion of 3-amino-7-(dimethylamino)phenothiazine-5-onium. It is used to prepare azure eosin staining agent for blood smears. It can also be used as a histological dye and a fluorescent dye. Its molecular structure contains 3-amino-7-(dimethylamino)phenothiazine-5-onium.
Azure A chloride is an organic chloride salt used as a DNA dye for electrophoresis and in azure eosin stains for blood smear staining. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C14H14CLN3S
|
|---|---|
| Molecular Weight |
291.80
|
| Exact Mass |
291.059
|
| CAS # |
531-53-3
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| PubChem CID |
13735
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| Appearance |
Brown to black solid powder
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| Boiling Point |
442.8ºC at 760 mmHg
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| Melting Point |
290 °C (dec.)(lit.)
|
| Flash Point |
221.6ºC
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
19
|
| Complexity |
483
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
NALREUIWICQLPS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
|
| Chemical Name |
(7-aminophenothiazin-3-ylidene)-dimethylazanium;chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: < 0.1 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4270 mL | 17.1350 mL | 34.2700 mL | |
| 5 mM | 0.6854 mL | 3.4270 mL | 6.8540 mL | |
| 10 mM | 0.3427 mL | 1.7135 mL | 3.4270 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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