| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Acrylodan targets thiol (-SH) groups on proteins, specifically cysteine residues. The compound does not target a specific protein or enzyme but rather serves as a covalent labeling reagent that attaches the environment-sensitive fluorophore to cysteine residues on proteins for studying protein structure and dynamics.
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|---|---|
| ln Vitro |
Guidelines (This is our suggested protocol; it should be adjusted based on your unique requirements as it just offers as a guideline). Act 1: 1. Purification and Labeling: 1. After adding a 20-fold molar excess of acrylodan (Anaspec) to the polymerized actin, it was let to sit on ice for an entire night. 2. After centrifuging 100,000 × g of labeled actin for two hours at 4 °C, the pellet was resuspended in 10 mm Tris buffer with pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, and 50 μm ATP. The mixture was subsequently dialyzed for seventy hours at 4 °C. 3. After collecting the supernatant, the extinction coefficients of 26,600 M 1 cm 1 for actin at 290 nm, 18,500 M 1 cm 1 for acrylodan at 385 nm, and 71,000 M 1 cm 1 for Alexa Fluor 488 at 494 nm were used to compute the actin content and degree of labeling. 4. The emission spectra of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1, differs significantly from that of Acrylodan-labeled Act1 (AcrylAct1NAT).
The in vitro activity of Acrylodan is its function as an environment-sensitive fluorescent probe for protein studies. The compound reacts with thiol groups on cysteine residues, forming a covalent bond. The fluorescence of Acrylodan is extremely sensitive to the local environment polarity and dynamics, changing in response to conformational changes in the labeled protein. This property makes it valuable for studying protein structural changes, protein-protein interactions, and protein folding. |
| ln Vivo |
Acrylodan is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving protein structural analysis. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro labeling assays for Acrylodan involve incubating the thiol-reactive probe with proteins containing free cysteine residues in appropriate buffer conditions. The reaction forms a covalent bond between the acryloyl group of Acrylodan and the thiol group of the cysteine residue. The labeled protein can then be analyzed by fluorescence spectroscopy to study conformational changes and environmental sensitivity.
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| Cell Assay |
For in vitro cellular experiments, Acrylodan is used to label proteins in cell lysates or purified preparations. The labeled proteins can be studied using fluorescence spectroscopy to investigate conformational changes and protein dynamics.
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| Animal Protocol |
In vivo animal experiments with Acrylodan are not typically performed, as the compound is primarily used as a research tool for in vitro protein structural studies. The compound is intended for research use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Acrylodan have not been characterized, as the compound is a research-use fluorescent probe rather than a drug candidate. The compound has a molecular weight of 225.29 g/mol and should be stored at 4°C, protected from light. It is a solid at room temperature.
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| Toxicity/Toxicokinetics |
Toxicological data for Acrylodan has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
Acrylodan is a thiol-reactive, environment-sensitive fluorophore used for site-directed protein labeling and conformational analysis. Its fluorescence is highly sensitive to conformational changes, making it valuable for protein structural studies. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C15H15NO
|
|---|---|
| Molecular Weight |
225.29
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| Exact Mass |
225.115
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| CAS # |
86636-92-2
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| PubChem CID |
104901
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.1g/cm3
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| Boiling Point |
388.3ºC at 760mmHg
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| Flash Point |
153.1ºC
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| Index of Refraction |
1.63
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| LogP |
3.274
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
17
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| Complexity |
297
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CN(C)C1=CC2=C(C=C1)C=C(C=C2)C(=O)C=C
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| InChi Key |
HMWAJFNEGAJETK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H15NO/c1-4-15(17)13-6-5-12-10-14(16(2)3)8-7-11(12)9-13/h4-10H,1H2,2-3H3
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| Chemical Name |
1-[6-(dimethylamino)naphthalen-2-yl]prop-2-en-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (221.94 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4387 mL | 22.1936 mL | 44.3872 mL | |
| 5 mM | 0.8877 mL | 4.4387 mL | 8.8774 mL | |
| 10 mM | 0.4439 mL | 2.2194 mL | 4.4387 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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