| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Alkyne-reactive groups (azides or iodoarenes) are the primary molecular targets. PYBG contains a terminal alkyne group and can also act as a substrate for Sonogashira coupling. The compound is used to conjugate with fluorescent dyes or other functional molecules.
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|---|---|
| ln Vitro |
In cell-free assays, PYBG is used as a flexible precursor for conjugation. Its activity is measured by the efficiency and yield of the conjugation reaction with an azide-functionalized fluorescent dye in the presence of a copper catalyst (CuSO4, ascorbate). The labeled conjugate can then be used as a fluorescent probe in biological assays.
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| ln Vivo |
There is no specific in vivo activity data available for this compound. PYBG itself is not a probe but a building block that becomes active after conjugation to a fluorescent reporter molecule. The resulting conjugates may be used for imaging or tracking in cells or animals.
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| Enzyme Assay |
A standard protocol involves dissolving PYBG (e.g., 1-10 mM) in a reaction solvent such as DMF or DMSO. For CuAAC conjugation, add an azide-functionalized fluorescent dye (e.g., 1-1.5 equivalents relative to PYBG), copper(I) catalyst or copper(II) sulfate with a reducing agent (e.g., sodium ascorbate), and a ligand (e.g., TBTA). Stir the reaction at room temperature or 37degC for 2-24 hours. Purify the conjugated product by HPLC.
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| Cell Assay |
A general protocol for cellular imaging would involve first conjugating PYBG to a fluorescent reporter via click chemistry, then purifying the conjugate. The resulting fluorescent probe can be used in cell-based assays at concentrations of 1-10 uM, with incubation times of 30-60 minutes at 37degC, followed by washing and confocal microscopy.
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| Animal Protocol |
There is no standard in vivo animal protocol for PYBG itself. Once conjugated to a fluorescent dye, the resulting probe can be administered intravenously or intraperitoneally to animal models for imaging studies. Typical doses for fluorescent probes range from 0.1-10 mg/kg.
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| ADME/Pharmacokinetics |
General PK properties for PYBG itself are not applicable. Once conjugated, the PK properties of the resulting fluorescent probe will depend on the nature of the conjugate (size, charge, hydrophobicity). Formulation is typically in DMSO or in aqueous buffers with co-solvents.
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| Toxicity/Toxicokinetics |
General toxicity for click chemistry precursors is considered low. At typical working concentrations, no significant toxicity is reported. Standard safety precautions for handling alkyne compounds and organic solvents should be observed.
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| References | |
| Additional Infomation |
PYBG is a research tool for bioconjugation and chemical biology. It is used as a flexible linker to attach various fluorescent dyes via click chemistry, enabling the creation of custom fluorescent probes for imaging, tracking, and detection of biomolecules. The propargyl ether linker provides stability and flexibility.
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| Molecular Formula |
C16H15N5O2
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|---|---|
| Molecular Weight |
309.323
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| Exact Mass |
309.122
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| CAS # |
680622-71-3
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| PubChem CID |
58596748
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| Appearance |
White to off-white solid powder
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| LogP |
1
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
23
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| Complexity |
418
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(OCC1=CC=C(COC2NC(N)=NC3C=2N=CN=3)C=C1)C#C
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| InChi Key |
LZDBKQCASZCNSH-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H15N5O2/c1-2-7-22-8-11-3-5-12(6-4-11)9-23-15-13-14(19-10-18-13)20-16(17)21-15/h1,3-6,10H,7-9H2,(H3,17,18,19,20,21)
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| Chemical Name |
6-[[4-(prop-2-ynoxymethyl)phenyl]methoxy]-7H-purin-2-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 6.25 mg/mL (20.21 mM)
H2O: < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2329 mL | 16.1645 mL | 32.3290 mL | |
| 5 mM | 0.6466 mL | 3.2329 mL | 6.4658 mL | |
| 10 mM | 0.3233 mL | 1.6164 mL | 3.2329 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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