| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Eosin Y does not target a specific protein or enzyme. As a histological dye, its utility in staining is based on its chemical and physical properties, particularly its affinity for eosinophilic (acidophilic) structures in tissues, including cytoplasmic proteins and extracellular matrix components. The dye interacts with cellular components through electrostatic and hydrophobic interactions.
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|---|---|
| ln Vitro |
The in vitro activity of Eosin Y is characterized by its function as a histological stain. The dye has an absorbance maximum at 523.0 nm and exhibits red fluorescence. Eosin Y is commonly paired with hematoxylin in H&E staining, which is the standard technique for revealing cell and tissue structure. Eosin Y stains cytoplasmic proteins, collagen, and muscle fibers, providing contrast for microscopic visualization. At concentrations of 10-50 μM, Eosin Y reduces the affinity of Mg²⁺-ATPase for ATP and increases the enzyme affinity for Mg²⁺.
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| ln Vivo |
Eosin Y is not used as a systemic therapeutic agent. Its in vivo utility is limited to diagnostic and research applications in histology and pathology for staining tissue sections. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro binding assays are not applicable for Eosin Y as it functions as a histological dye rather than a drug targeting specific enzymes or receptors. The compound is used in staining protocols where it is applied directly to tissue sections to provide contrast for microscopic visualization.
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| Cell Assay |
For in vitro cellular experiments, Eosin Y is used in histological staining procedures. Tissue sections are first stained with hematoxylin to stain nuclei, followed by counterstaining with Eosin Y to stain cytoplasmic proteins and extracellular matrix. After appropriate washing and dehydration steps, the stained samples are examined under a light microscope. The dye provides contrast for the visualization of cellular and tissue structures.
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| Animal Protocol |
In vivo animal experiments with Eosin Y are not typically performed, as the compound is primarily used as an in vitro histological stain for tissue sections. The compound is intended for research and diagnostic use only.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Eosin Y have not been characterized, as the compound is a research-use histological dye rather than a drug candidate. The compound has a molecular weight of 647.89 g/mol and should be stored at 15-30°C. The dye content is approximately 99%.
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| Toxicity/Toxicokinetics |
Toxicological data for Eosin Y has not been systematically evaluated for therapeutic use, as the compound is intended for research and diagnostic applications only. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
Bromine is an organic bromine compound whose function is related to fluorescein (lactone form).
Eosin Y is a brominated derivative of fluorescein and a red fluorescent dye from the xanthene family. It is widely used in hematoxylin and eosin (H&E) staining to stain cytoplasmic proteins and extracellular matrix. Eosin Y is a cost-effective and widely available dye that serves as a photoredox catalyst in organic synthesis. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C20H8BR4O5
|
|---|---|
| Molecular Weight |
647.89
|
| Exact Mass |
687.674
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| CAS # |
15086-94-9
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| Related CAS # |
Eosin Y disodium;17372-87-1
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| PubChem CID |
27020
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| Appearance |
Yellow to orange solid powder
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| Density |
1.02 g/mL at 20ºC
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| Boiling Point |
640.3ºC at 760 mmHg
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| Melting Point |
295ºC
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| Index of Refraction |
1.757
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| LogP |
6.122
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
29
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| Complexity |
645
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)Br)O)Br)Br)O)Br
|
| InChi Key |
DBZJJPROPLPMSN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C20H8Br4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H
|
| Chemical Name |
2',4',5',7'-tetrabromo-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (385.87 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5435 mL | 7.7174 mL | 15.4347 mL | |
| 5 mM | 0.3087 mL | 1.5435 mL | 3.0869 mL | |
| 10 mM | 0.1543 mL | 0.7717 mL | 1.5435 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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