| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
5-Bromo-3-indolyl β-D-galactopyranoside targets the enzyme β-galactosidase (β-gal), serving as a chromogenic substrate for the detection of β-galactosidase activity. The compound is specifically designed to be cleaved by this enzyme, releasing an indoxyl intermediate that forms an insoluble indigo-colored precipitate.
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|---|---|
| ln Vitro |
The in vitro activity of Bluo-Gal is its function as a chromogenic substrate for β-galactosidase. Upon enzymatic cleavage, the substrate releases a 5-bromo-3-indolyl intermediate that undergoes oxidative dimerization to form an insoluble indigo-colored precipitate (5,5'-dibromo-4,4'-dichloro-indigo) at the site of enzyme activity. This blue precipitate marks the location of β-galactosidase activity, enabling the detection and localization of the enzyme in biological samples. The compound is cell-permeable, allowing for detection of intracellular β-galactosidase activity.
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| ln Vivo |
Bluo-Gal is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the detection of β-galactosidase reporter gene expression in transgenic organisms. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro enzyme assays for Bluo-Gal involve incubating tissue samples, cellular extracts, or bacterial cultures with the chromogenic substrate in appropriate buffer conditions. β-Galactosidase cleaves the substrate, releasing an indoxyl intermediate that forms an insoluble indigo-colored precipitate at the site of enzyme activity. The appearance of blue color indicates the presence of β-galactosidase activity. The staining intensity can be assessed visually or quantified by measuring the absorbance of extracted dye.
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| Cell Assay |
For in vitro cellular experiments, Bluo-Gal is used in histochemical staining procedures for the detection of β-galactosidase reporter gene expression. Tissue sections, whole-mount preparations, or cell cultures are incubated with the substrate, and the resulting indigo-colored precipitate indicates the location of β-galactosidase activity. The staining is cell-permeable, allowing detection of intracellular enzyme activity.
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| Animal Protocol |
For in vivo animal experiments, Bluo-Gal has been used to detect the expression of β-galactosidase in muscle from transgenic mice expressing E. coli lacZ. The compound can be administered to tissues or whole organisms for histochemical detection of β-galactosidase activity. However, systemic administration is not typically performed.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Bluo-Gal have not been characterized, as the compound is a research-use chromogenic substrate rather than a drug candidate. The compound has a molecular weight of 374.18 g/mol and should be stored under appropriate conditions, protected from light.
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| Toxicity/Toxicokinetics |
Toxicological data for Bluo-Gal has not been systematically evaluated, as the compound is intended for research applications only and not for therapeutic use. The compound appears as a white to almost white powder to crystal and is intended for biochemical research use only. Standard laboratory safety precautions should be observed.
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| References | |
| Additional Infomation |
β-galactosidase substrate detector
Bluo-Gal (5-Bromo-3-indolyl β-D-galactopyranoside) is a cell-permeable chromogenic substrate for β-galactosidase that produces an indigo-colored precipitate upon enzymatic hydrolysis. It is used to detect bacterial β-galactosidase activity and β-galactosidase reporter gene expression. The compound has been used in transgenic mouse studies and has no clinical or therapeutic applications. |
| Molecular Formula |
C14H16BRNO6
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|---|---|
| Molecular Weight |
374.18
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| Exact Mass |
373.016
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| CAS # |
97753-82-7
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| PubChem CID |
126898
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| Appearance |
White to off-white solid powder
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| Density |
1.8±0.1 g/cm3
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| Boiling Point |
646.6±55.0 °C at 760 mmHg
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| Flash Point |
344.9±31.5 °C
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| Vapour Pressure |
0.0±2.0 mmHg at 25°C
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| Index of Refraction |
1.731
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| LogP |
0.11
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
388
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| Defined Atom Stereocenter Count |
5
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| SMILES |
C1=CC2=C(C=C1Br)C(=CN2)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O
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| InChi Key |
LINMATFDVHBYOS-MBJXGIAVSA-N
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| InChi Code |
InChI=1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
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| Chemical Name |
(2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 125 mg/mL (334.06 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6725 mL | 13.3626 mL | 26.7251 mL | |
| 5 mM | 0.5345 mL | 2.6725 mL | 5.3450 mL | |
| 10 mM | 0.2673 mL | 1.3363 mL | 2.6725 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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