| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
Purity: ≥98%
| Targets |
ER-Tracker Green targets the endoplasmic reticulum (ER) through its glibenclamide moiety. Glibenclamide is a sulfonylurea that binds to sulfonylurea receptors, which are associated with ATP-sensitive potassium channels. However, in the context of ER-Tracker Green, the glibenclamide moiety provides specificity for the ER. The BODIPY fluorophore provides green fluorescence (Ex/Em=504/511 nm). The compound is cell-permeable and has low cytotoxicity at low concentrations.
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| ln Vitro |
1. The ER-Tracker working solution is prepared as follows: 1.1 Stock solution preparation: dissolve 100 μg of ER-Tracker in 128 μL DMSO to create a 1 mM stock solution. Note: It is advised to keep the stock solution frozen and to avoid repeatedly thawing and freezing it at -20°C or -80°C in the dark. 1.2 Making the working solution for ER-Tracker Dilute the stock solution to a concentration of 100 nM-1 μM using either PBS or serum-free cell culture medium. It is important that you modify the ER-Tracker working fluid concentration based on the current circumstances. 2. Suspension cells in a 6-well plate (2.1) are stained A. After three to five minutes at 4°C and 1000 g of centrifugation, discard the supernatant. Wash for five minutes each time, twice, using PBS. One x 10^6 cells per milliliter. B. After adding 1 mL of the working solution, let it sit at room temperature for five to thirty minutes. an. Discard the supernatant after centrifuging for three to four minutes at 400 g and 4°C. D. Wash for five minutes each time, twice, using PBS. E. In PBS or serum-free cell culture media, resuspend the cells. either flow cytometry monitoring or fluorescence microscopy. Cells that adhere 2.2 a. Adherent cells are cultured on sterile coverslips. B. Lift the coverslip out of the culture media and use the aspirator to remove any extra. an. To fully cover the cells, add 100 μL of working solution, shake gently, and then incubate for five to thirty minutes at room temperature. D. Use medium for five minutes each time you wash. either flow cytometry monitoring or fluorescence microscopy. Note: Cells must be resuspended prior to staining if flow cytometry is being used for detection.
The in vitro activity of ER-Tracker Green is its function as a selective ER stain for live cell imaging. The dye is a BODIPY-based fluorescent probe conjugated with glibenclamide, providing high selectivity for the endoplasmic reticulum. It has excitation/emission maxima of 504/511 nm. The dye is cell-permeable and has low cytotoxicity at low concentrations. It is an environmentally sensitive probe and can retain some fluorescence after formaldehyde treatment. |
| ln Vivo |
ER-Tracker Green is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the labeling of the endoplasmic reticulum in live cells. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro ER staining assays for ER-Tracker Green involve incubating live cells with the dye in cell culture medium at low concentrations. The cell-permeable dye enters cells and selectively stains the endoplasmic reticulum. After incubation, cells are washed and analyzed by fluorescence microscopy with excitation at 504 nm and emission at 511 nm. The dye is suitable for live cell imaging and can be used for time-lapse studies of ER dynamics. The dye can also be used after fixation, as some fluorescence is retained after formaldehyde treatment.
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| Cell Assay |
For in vitro cellular experiments, ER-Tracker Green is dissolved in DMSO and diluted in cell culture medium. Cells are incubated with the dye for a defined period, washed, and then analyzed by fluorescence microscopy. The green fluorescence (Ex/Em=504/511 nm) provides visualization of the endoplasmic reticulum structure and dynamics in live cells.
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| Animal Protocol |
In vivo animal experiments with ER-Tracker Green are not typically performed, as the compound is primarily used as a cell culture reagent.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of ER-Tracker Green have not been characterized, as the compound is a research-use fluorescent dye rather than a drug candidate. The compound has a molecular weight of 783.09 g/mol and should be stored at -20°C.
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| Toxicity/Toxicokinetics |
ER-Tracker Green is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References |
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| Additional Infomation |
ER-Tracker Green (CAS#: 730931-46-1) is a cell-permeable fluorescent dye that specifically stains the endoplasmic reticulum in live cells. It is a BODIPY-based probe conjugated with glibenclamide with excitation/emission at 504/511 nm. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C37H42BCLF2N6O6S
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| Molecular Weight |
783.09179353714
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| Exact Mass |
782.263
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| CAS # |
730931-46-1
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| PubChem CID |
168008655
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| Appearance |
Brown to reddish brown solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
54
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| Complexity |
1610
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ClC1C=C(C(NCCC2C=CC(=CC=2)S(NC(NC2CCCCC2)=O)(=O)=O)=O)C(=C(C=1)NC(CCC1C=CC2=CC3=C(C)C=C(C)N3[B-]([N+]2=1)(F)F)=O)OC
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| InChi Key |
DOQZRTREFZBLMY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C37H42BClF2N6O6S/c1-23-19-24(2)46-33(23)22-29-12-11-28(47(29)38(46,40)41)13-16-34(48)44-32-21-26(39)20-31(35(32)53-3)36(49)42-18-17-25-9-14-30(15-10-25)54(51,52)45-37(50)43-27-7-5-4-6-8-27/h9-12,14-15,19-22,27H,4-8,13,16-18H2,1-3H3,(H,42,49)(H,44,48)(H2,43,45,50)
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| Chemical Name |
5-chloro-N-[2-[4-(cyclohexylcarbamoylsulfamoyl)phenyl]ethyl]-3-[3-(2,2-difluoro-10,12-dimethyl-1-aza-3-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-3,5,7,9,11-pentaen-4-yl)propanoylamino]-2-methoxybenzamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50 mg/mL (63.85 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2770 mL | 6.3850 mL | 12.7699 mL | |
| 5 mM | 0.2554 mL | 1.2770 mL | 2.5540 mL | |
| 10 mM | 0.1277 mL | 0.6385 mL | 1.2770 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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