| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
Purity: =97.89%
| Targets |
The azide functional group on TAMRA azide targets alkyne, DBCO, and BCN-functionalized molecules through click chemistry reactions. The compound does not target a specific protein or enzyme but rather serves as a fluorescent labeling reagent that attaches the TAMRA fluorophore to azide-reactive biomolecules. TAMRA (tetramethylrhodamine) is an orange fluorescent dye commonly used as a FRET acceptor for FAM. The 5-isomer provides a pure, well-defined product for consistent labeling results.
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| ln Vitro |
The in vitro activity of TAMRA azide is its function as a click chemistry reagent for fluorescent labeling. The azide group undergoes copper-catalyzed click reactions with alkynes, DBCO, and BCN to form stable triazole linkages. The TAMRA fluorophore provides orange fluorescence, making the labeled conjugates detectable by fluorescence microscopy, flow cytometry, or other fluorescence-based methods. TAMRA is often used as a FRET acceptor for the FAM fluorophore.
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| ln Vivo |
In vivo, TAMRA azide is not used as a therapeutic agent. Its utility is limited to research applications involving the fluorescent labeling of biomolecules via click chemistry.
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| Enzyme Assay |
In vitro labeling assays for TAMRA azide involve incubating the azide with alkyne, DBCO, or BCN-functionalized biomolecules under appropriate click chemistry reaction conditions (copper-catalyzed for alkynes or copper-free for DBCO/BCN). The reaction forms a stable triazole linkage between the azide and the alkyne/cyclooctyne. Labeling efficiency can be assessed by measuring the fluorescence intensity of the conjugated product using fluorescence spectroscopy.
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| Cell Assay |
For in vitro cellular experiments, TAMRA azide is used in click chemistry-based labeling of cellular components. Cells are first incubated with alkyne-functionalized probes, then fixed and permeabilized. TAMRA azide is added along with copper catalyst (for CuAAC) to label the alkyne-containing molecules. The orange fluorescence can be detected by fluorescence microscopy or flow cytometry.
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| Animal Protocol |
In vivo animal experiments with TAMRA azide are not typically performed, as the compound is primarily used as an in vitro labeling reagent.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of TAMRA azide have not been characterized, as the compound is a research-use fluorescent labeling reagent rather than a drug candidate. The compound has a molecular weight of 512.56 g/mol and should be stored at -20°C.
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| Toxicity/Toxicokinetics |
TAMRA azide is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
TAMRA azide, 5-isomer (CAS#: 825651-66-9) is a click chemistry reagent with an azide group for linking to the orange dye TAMRA. TAMRA is often used as a FRET acceptor for FAM. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C28H28N6O4
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|---|---|
| Molecular Weight |
512.56
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| Exact Mass |
512.217
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| CAS # |
825651-66-9
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| PubChem CID |
163304583
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| Appearance |
Brown to reddish brown solid powder
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| LogP |
4.4
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
38
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| Complexity |
1040
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
UJIRBDGHKAQZRJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C28H30N6O4/c1-33(2)17-9-11-20-23(15-17)37-24-16-18(34(3)4)10-12-21(24)28(20)22-8-5-7-19(25(22)27(36)38-28)26(35)30-13-6-14-31-32-29/h5,7-12,15-16,20,23H,6,13-14H2,1-4H3,(H,30,35)
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| Chemical Name |
N-(3-azidopropyl)-3',6'-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9'-4a,9a-dihydroxanthene]-4-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 125 mg/mL (243.87 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9510 mL | 9.7550 mL | 19.5099 mL | |
| 5 mM | 0.3902 mL | 1.9510 mL | 3.9020 mL | |
| 10 mM | 0.1951 mL | 0.9755 mL | 1.9510 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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