| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| Other Sizes |
| Targets |
The azide functional group on FAM azide targets alkyne-functionalized molecules through copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. The compound does not target a specific protein or enzyme but rather serves as a fluorescent labeling reagent that attaches the FAM fluorophore to alkyne-containing biomolecules. FAM (fluorescein) is a widely used green fluorescent dye. The 6-isomer provides a pure, well-defined product for consistent labeling results.
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| ln Vitro |
The in vitro activity of FAM azide is its function as a click chemistry reagent for fluorescent labeling. The azide group undergoes copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules to form a stable triazole linkage. The FAM fluorophore provides green fluorescence, making the labeled conjugates detectable by fluorescence microscopy, flow cytometry, or other fluorescence-based methods. The compound has been used to label 5-ethynyl-2'-deoxyuridine (EdU) for cell viability studies.
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| ln Vivo |
FAM azide is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the fluorescent labeling of biomolecules. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro labeling assays for FAM azide involve incubating the azide with alkyne-functionalized biomolecules in the presence of copper catalyst and a reducing agent (CuAAC conditions). The reaction forms a stable triazole linkage between the azide and alkyne groups. Labeling efficiency can be assessed by measuring the fluorescence intensity of the conjugated product using fluorescence spectroscopy, flow cytometry, or fluorescence microscopy.
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| Cell Assay |
For in vitro cellular experiments, FAM azide is used in click chemistry-based labeling of cellular components. Cells are first incubated with alkyne-functionalized probes (such as EdU for DNA synthesis detection), then fixed and permeabilized. FAM azide is added along with copper catalyst to label the alkyne-containing molecules. The green fluorescence can be detected by fluorescence microscopy or flow cytometry. This method is commonly used for cell proliferation assays and other cellular studies.
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| Animal Protocol |
In vivo animal experiments with FAM azide are not typically performed, as the compound is primarily used as an in vitro labeling reagent. However, the compound could potentially be used for in vivo imaging applications if conjugated to targeting ligands via click chemistry.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of FAM azide have not been characterized, as the compound is a research-use fluorescent labeling reagent rather than a drug candidate. The compound has a molecular weight of 458.42 g/mol and should be stored at -20°C, protected from light.
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| Toxicity/Toxicokinetics |
FAM azide is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
FAM azide, 6-isomer (CAS#: 1386385-76-7) is a fluorescent dye and click chemistry reagent containing an azide group. It is used for labeling alkyne-functionalized targets and has been used to label EdU for cell viability studies. The compound has no clinical or therapeutic applications.
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| Molecular Formula |
C24H18N4O6
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|---|---|
| Molecular Weight |
458.423
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| Exact Mass |
458.122
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| CAS # |
1386385-76-7
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| PubChem CID |
71581527
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
4
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
34
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| Complexity |
821
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C2=CC=C(C(=O)NCCCN=[N+]=[N-])C=C2C2(C3=CC=C(O)C=C3OC3=CC(O)=CC=C23)O1
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| InChi Key |
JQDJGCPZDAATOX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C24H18N4O6/c25-28-27-9-1-8-26-22(31)13-2-5-16-19(10-13)24(34-23(16)32)17-6-3-14(29)11-20(17)33-21-12-15(30)4-7-18(21)24/h2-7,10-12,29-30H,1,8-9H2,(H,26,31)
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| Chemical Name |
N-(3-azidopropyl)-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (218.14 mM)
Methanol: 5 mg/mL (10.91 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1814 mL | 10.9070 mL | 21.8141 mL | |
| 5 mM | 0.4363 mL | 2.1814 mL | 4.3628 mL | |
| 10 mM | 0.2181 mL | 1.0907 mL | 2.1814 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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