| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Membrane proteins and lipid bilayers are the primary targets. This detergent is used to solubilize and stabilize membrane proteins for biochemical and structural studies without denaturing them. It is classified as a non-ionic surfactant.
|
|---|---|
| ln Vitro |
The activity of Octyl alpha-D-glucopyranoside is characterized by its critical micelle concentration (typically around 20-25 mM). Its ability to solubilize membrane proteins from lipid bilayers can be assessed by protein yield and retention of functional activity (e.g., ligand binding or enzymatic activity) in solubilized extracts.
|
| ln Vivo |
There is no specific in vivo activity reported for this compound. It is used exclusively as an in vitro biochemical reagent for protein extraction and purification, rather than as a therapeutic or probe for animal studies.
|
| Enzyme Assay |
There is no specific enzyme/receptor binding protocol for this compound. A standard protocol for protein solubilization involves adding Octyl alpha-D-glucopyranoside (e.g., 1-2% w/v or 30-60 mM) to a suspension of membrane-containing material (e.g., cell lysate or isolated membranes) in a buffer (e.g., 20 mM Tris, pH 8.0, 100-300 mM NaCl), incubating on ice for 30-60 minutes, then centrifuging to separate solubilized proteins from insoluble debris.
|
| Cell Assay |
There is no specific cell-based protocol for this compound. In cell lysis protocols for membrane protein extraction, Octyl alpha-D-glucopyranoside can be added directly to cell pellets at a final concentration of 0.5-2% (w/v) in a lysis buffer containing protease inhibitors, followed by incubation on ice and centrifugation.
|
| Animal Protocol |
There is no standard in vivo animal protocol for this compound. It is not intended for use in live animals, as it is a detergent used in vitro.
|
| ADME/Pharmacokinetics |
General PK properties for this compound are not applicable. As a nonionic detergent, it is not used as a drug. Its solubility is high in water, and it can form micelles at concentrations above its CMC. Formulation is typically as a solid or in aqueous buffers.
|
| Toxicity/Toxicokinetics |
General toxicity for nonionic detergents is considered moderate. At working concentrations (0.1-2%), Octyl alpha-D-glucopyranoside can be cytotoxic to cells if used in vivo, but in biochemical applications, it is considered safe with proper handling. Standard lab safety precautions (gloves, goggles) should be used.
|
| Additional Infomation |
Octyl α-D-glucopyranoside is an α-D-glucopyranoside in which the terminal hydrogen of α-D-glucopyranose is replaced by an octyl group.
Octyl alpha-D-glucopyranoside is a research-grade nonionic detergent commonly used in membrane protein biochemistry for the extraction, solubilization, and purification of membrane proteins from various sources. It is often used as an alternative to more harsh detergents because it is milder. This product is for research use only. |
| Molecular Formula |
C14H28O6
|
|---|---|
| Molecular Weight |
292.37
|
| Exact Mass |
292.189
|
| CAS # |
29781-80-4
|
| PubChem CID |
445318
|
| Appearance |
White to off-white solid powder
|
| Density |
1.18 g/cm3
|
| Boiling Point |
454.1ºC at 760 mmHg
|
| Melting Point |
110-112ºC
|
| Flash Point |
228.4ºC
|
| Vapour Pressure |
3.72E-10mmHg at 25°C
|
| Index of Refraction |
1.515
|
| LogP |
0.163
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
9
|
| Heavy Atom Count |
20
|
| Complexity |
250
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
O1[C@@]([H])([C@@]([H])([C@]([H])([C@@]([H])([C@@]1([H])C([H])([H])O[H])O[H])O[H])O[H])OC([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H]
|
| InChi Key |
HEGSGKPQLMEBJL-RGDJUOJXSA-N
|
| InChi Code |
InChI=1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14+/m1/s1
|
| Chemical Name |
(2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-octoxyoxane-3,4,5-triol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~342.0 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.55 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.55 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (8.55 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4203 mL | 17.1016 mL | 34.2032 mL | |
| 5 mM | 0.6841 mL | 3.4203 mL | 6.8406 mL | |
| 10 mM | 0.3420 mL | 1.7102 mL | 3.4203 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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