| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Dehydroergosterol does not target a specific protein or enzyme as a pharmacological agent. Instead, it functions as a fluorescent cholesterol analog that incorporates into cellular membranes and mimics the behavior of natural cholesterol. The compound can be used to monitor sterol uptake and sterol flux between organelles. Its interaction with membranes is based on its lipophilic properties and structural similarity to cholesterol, allowing it to partition into lipid bilayers and associate with cholesterol-binding proteins.
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| ln Vitro |
The in vitro activity of Dehydroergosterol is its function as a fluorescent probe for studying cholesterol dynamics. The compound mimics natural cholesterol in membrane incorporation and transport behavior. It can be used to monitor sterol uptake and sterol flux between organelles in live cells. The fluorescent properties (ex/em = 324/375 nm) enable the tracking of cholesterol in cellular membranes, lipoproteins, and organelles involved in lipid homeostasis. Dehydroergosterol plays a crucial role in various biochemical reactions, primarily due to its ability to mimic cholesterol, and it interacts with several enzymes.
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| ln Vivo |
Dehydroergosterol is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the study of cholesterol trafficking and lipid metabolism. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro assays for Dehydroergosterol involve incubating cells with the fluorescent cholesterol analog. The compound is taken up by cells and incorporated into membranes, mimicking natural cholesterol behavior. The fluorescence can be detected by fluorescence microscopy or flow cytometry to study cholesterol distribution, trafficking, and dynamics in cells. The compound is readily bound by cholesterol-binding proteins.
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| Cell Assay |
For in vitro cellular experiments, Dehydroergosterol is dissolved in ethanol (20 mg/ml) or other suitable solvents and added to cell culture medium. Cells are incubated with the compound for a defined period, washed, and then analyzed by fluorescence microscopy. The fluorescent cholesterol analog allows for the visualization of sterol movement and lipid domains in live cells. It can be used to study cholesterol uptake, intracellular transport, and organelle distribution.
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| Animal Protocol |
For in vivo animal experiments, Dehydroergosterol can be used to study cholesterol metabolism and trafficking. The compound can be administered to animal models, and its distribution can be tracked by fluorescence imaging. However, such studies are not commonly reported.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Dehydroergosterol have not been extensively characterized, as the compound is a research-use fluorescent probe rather than a drug candidate. The compound has a molecular weight of 394.64 g/mol and is soluble in ethanol (20 mg/ml), DMF (2 mg/ml), and DMSO (0.1 mg/ml). It should be stored at -20°C and shipped at room temperature in continental US.
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| Toxicity/Toxicokinetics |
Dehydroergosterol is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
Dehydroergosterol is a phytosterol composed of ergosterane, with double bonds at positions 5, 6, 7, 8, 9, 11, and 22, 23, and a 3β-hydroxyl group. It can be used as both a biomarker and a metabolite. It is an ergosterane compound, a 3β-sterol, a phytosterol compound, and also a 3β-hydroxy-Δ5-steroid. Dehydroergosterol has been reported in Eutypa lata, Phycomyces blakesleeanus, and other organisms with relevant data. Dehydroergosterol is a metabolite found in or produced by Saccharomyces cerevisiae.
Dehydroergosterol (DHE) (CAS#: 516-85-8) is a naturally occurring fluorescent sterol analog of cholesterol (ex/em = 324/375 nm) that mimics the properties of cholesterol in cell membranes. It is used for real-time probing of the sterol environment and to elucidate intracellular sterol trafficking. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C28H42O
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|---|---|
| Molecular Weight |
394.63
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| Exact Mass |
394.324
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| CAS # |
516-85-8
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| PubChem CID |
6436660
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.02g/cm3
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| Boiling Point |
520.6ºC at 760 mmHg
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| Melting Point |
146°C (lit.)
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| Flash Point |
221ºC
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| Index of Refraction |
1.553
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| LogP |
7.25
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
29
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| Complexity |
765
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| Defined Atom Stereocenter Count |
7
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| SMILES |
CC(C)[C@@H](C)/C=C/[C@@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@H](CC[C@]4(C)C3=CC[C@]12C)O
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| InChi Key |
QSVJYFLQYMVBDR-CMNOFMQQSA-N
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| InChi Code |
InChI=1S/C28H42O/c1-18(2)19(3)7-8-20(4)24-11-12-25-23-10-9-21-17-22(29)13-15-27(21,5)26(23)14-16-28(24,25)6/h7-10,14,18-20,22,24-25,29H,11-13,15-17H2,1-6H3/b8-7+/t19-,20+,22-,24+,25-,27-,28+/m0/s1
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| Chemical Name |
(3S,10S,13R,14R,17R)-17-[(E,2R,5R)-5,6-dimethylhept-3-en-2-yl]-10,13-dimethyl-2,3,4,12,14,15,16,17-octahydro-1H-cyclopenta[a]phenanthren-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5340 mL | 12.6701 mL | 25.3402 mL | |
| 5 mM | 0.5068 mL | 2.5340 mL | 5.0680 mL | |
| 10 mM | 0.2534 mL | 1.2670 mL | 2.5340 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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