| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g | |||
| Other Sizes |
| Targets |
Resorufin-β-D-galactopyranoside targets the enzyme β-galactosidase (β-Gal), serving as a fluorogenic substrate for the detection of β-galactosidase activity. The compound is specifically designed to be cleaved by this enzyme, releasing the fluorescent resorufin moiety upon enzymatic hydrolysis. This makes it a valuable tool for studying gene expression, protein localization, and cellular processes where β-galactosidase is used as a reporter enzyme.
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| ln Vitro |
The in vitro activity of Resorufin-β-D-galactopyranoside is its function as a fluorogenic substrate for β-galactosidase. The compound is non-fluorescent in its intact form. Upon enzymatic cleavage by β-galactosidase, the fluorescent moiety resorufin is released, and its fluorescence can be used to quantify β-galactosidase activity. The fluorescence is measured with excitation at 571 nm and emission at 590 nm. The substrate provides a robust, selective, and sensitive solution for enzymatic assays, allowing simplified sample preparation and reliable quantification. It is used in reporter assays and enzyme activity measurements, and supports use in diverse research areas including microbiology, oncology, and developmental biology.
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| ln Vivo |
Resorufin-β-D-galactopyranoside is not used as a therapeutic agent. Its in vivo utility is limited to research applications involving the measurement of β-galactosidase activity in biological samples. The compound is not intended for systemic therapeutic use.
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| Enzyme Assay |
In vitro enzyme assays for Resorufin-β-D-galactopyranoside involve incubating the fluorogenic substrate with β-galactosidase enzyme preparations in appropriate buffer conditions. The enzyme cleaves the substrate, releasing fluorescent resorufin, which is measured fluorimetrically with excitation at 571 nm and emission at 590 nm. The fluorescence intensity is proportional to β-galactosidase activity. The assay is robust, selective, and sensitive, allowing simplified sample preparation and reliable quantification.
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| Cell Assay |
For in vitro cellular experiments, Resorufin-β-D-galactopyranoside is typically used in cell lysates or cell-free systems for enzyme activity assays. The substrate can be added to cell lysates or purified enzyme preparations to assess β-galactosidase activity. The fluorogenic readout provides a sensitive and quantitative measure of enzyme activity. The compound can also be used in live-cell imaging applications where β-galactosidase expression is monitored.
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| Animal Protocol |
In vivo animal experiments with Resorufin-β-D-galactopyranoside are not typically performed, as the compound is used as an in vitro enzyme substrate. However, the substrate can be used to measure β-galactosidase activity in tissue samples collected from animal models expressing the reporter enzyme.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Resorufin-β-D-galactopyranoside have not been characterized, as it is a research-use enzyme substrate rather than a drug candidate. The compound has a molecular weight of 375.33 g/mol and a LogP of -0.7. It is soluble in DMSO and should be stored as powder at -20°C for up to 3 years or at 4°C for up to 2 years.
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| Toxicity/Toxicokinetics |
Resorufin-β-D-galactopyranoside is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| Additional Infomation |
Fluorescent substrates for β-galactosidase activity
Resorufin-β-D-galactopyranoside (CAS#: 95079-19-9) is a fluorogenic substrate for β-galactosidase that releases fluorescent resorufin upon enzymatic cleavage. It is used in reporter assays, enzyme activity measurements, and environmental water analysis for estrogenicity screening. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C18H17NO8
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|---|---|
| Molecular Weight |
375.33
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| Exact Mass |
375.095
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| CAS # |
95079-19-9
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| PubChem CID |
125273
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.71 g/cm3
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| Boiling Point |
651.6ºCat 760 mmHg
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| Flash Point |
347.9ºC
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| Index of Refraction |
1.723
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| LogP |
-0.7
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
27
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| Complexity |
689
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| Defined Atom Stereocenter Count |
5
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| SMILES |
O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)C1C=CC2N=C3C=CC(=O)C=C3OC=2C=1
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| InChi Key |
QULZFZMEBOATFS-DISONHOPSA-N
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| InChi Code |
InChI=1S/C18H17NO8/c20-7-14-15(22)16(23)17(24)18(27-14)25-9-2-4-11-13(6-9)26-12-5-8(21)1-3-10(12)19-11/h1-6,14-18,20,22-24H,7H2/t14-,15+,16+,17-,18-/m1/s1
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| Chemical Name |
7-[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenoxazin-3-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6643 mL | 13.3216 mL | 26.6432 mL | |
| 5 mM | 0.5329 mL | 2.6643 mL | 5.3286 mL | |
| 10 mM | 0.2664 mL | 1.3322 mL | 2.6643 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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