| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| 5g |
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| 10g |
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| Other Sizes |
| Targets |
N-Acetyl-DL-penicillamine targets heavy metal ions, particularly methyl mercury, as a chelating agent. The compound binds to heavy metal ions, forming stable complexes that can be excreted from the body. At a concentration of 1 mM, it inhibits the binding of methyl mercury to isolated human erythrocytes by 50% and removes 50% of methyl mercury ions from methyl mercury-loaded blood cells.
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|---|---|
| ln Vitro |
The in vitro activity of N-Acetyl-DL-penicillamine is its function as a chelating agent. At a concentration of 1 mM, it inhibits the binding of methyl mercury to isolated human erythrocytes by 50% and removes 50% of methyl mercury ions from methyl mercury-loaded blood cells. The compound can be used in studies of heavy metal chelation and detoxification.
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| ln Vivo |
N-Acetyl-DL-penicillamine has demonstrated in vitro activity as a chelating agent for methyl mercury. No significant in vivo activity has been reported as a therapeutic agent, though penicillamine derivatives are used clinically for heavy metal poisoning.
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| Enzyme Assay |
In vitro heavy metal binding assays for N-Acetyl-DL-penicillamine involve incubating the chelating agent with heavy metal ions such as methyl mercury in appropriate buffer conditions. The binding of the compound to metal ions can be measured by various analytical techniques. The inhibition of methyl mercury binding to erythrocytes can be assessed by measuring the amount of mercury bound to cells in the presence and absence of the compound.
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| Cell Assay |
For in vitro cellular experiments, N-Acetyl-DL-penicillamine can be used to study the effects of heavy metal chelation on cellular function. Erythrocytes or other cell types can be treated with the compound in the presence of heavy metals to assess protection against metal toxicity.
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| Animal Protocol |
In vivo animal experiments with N-Acetyl-DL-penicillamine may be performed to study its effects on heavy metal detoxification. However, such studies are not commonly reported for this specific compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of N-Acetyl-DL-penicillamine have not been extensively characterized. The compound has a molecular weight of 191.25 g/mol, a melting point of 186-191°C (dec.), and a density of 1.2. It has a purity of 95%.
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| Toxicity/Toxicokinetics |
The compound is intended for research use only and not for human therapeutic or diagnostic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| Additional Infomation |
See also: N-acetyl-D-penicillamine (note moved to).
N-Acetyl-DL-penicillamine is a chelating agent that inhibits the binding of methyl mercury to erythrocytes and removes methyl mercury ions from blood cells at 1 mM. The compound has no clinical or therapeutic applications. |
| Molecular Formula |
C7H13NO3S
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|---|---|
| Molecular Weight |
191.25
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| Exact Mass |
191.062
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| CAS # |
59-53-0
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| PubChem CID |
27372
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| Appearance |
White to off-white solid powder
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| Density |
1.201g/cm3
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| Boiling Point |
392.7ºC at 760mmHg
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| Melting Point |
186-189ºC (dec.)(lit.)
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| Flash Point |
191.3ºC
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| LogP |
0.675
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
12
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| Complexity |
203
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)NC(C(=O)O)C(C)(C)S
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| InChi Key |
MNNBCKASUFBXCO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H13NO3S/c1-4(9)8-5(6(10)11)7(2,3)12/h5,12H,1-3H3,(H,8,9)(H,10,11)
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| Chemical Name |
2-acetamido-3-methyl-3-sulfanylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.2288 mL | 26.1438 mL | 52.2876 mL | |
| 5 mM | 1.0458 mL | 5.2288 mL | 10.4575 mL | |
| 10 mM | 0.5229 mL | 2.6144 mL | 5.2288 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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