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MNP-Glc (2-Methoxy-4-(2-nitroethenyl)phenyl β-D-glucopyranoside)

MNP-Glc is a biochemical compound that may be utilized as a biomaterial or organic/chemical reagent for biomedical research.
MNP-Glc (2-Methoxy-4-(2-nitroethenyl)phenyl β-D-glucopyranoside)
MNP-Glc (2-Methoxy-4-(2-nitroethenyl)phenyl β-D-glucopyranoside) Chemical Structure CAS No.: 70622-80-9
Product category: Biochemical Assay Reagents
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
25mg
250mg
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Product Description
MNP-Glc is a biochemical compound that may be utilized as a biomaterial or organic/chemical reagent for biomedical research.
MNP-Glc is a biochemical reagent that can be used as a biological material or organic compound for life science research. Its full name is 2-Methoxy-4-(2-nitroethenyl)phenyl beta-D-glucopyranoside (CAS: 70622-80-9). The compound has a molecular weight of 357.31 and the formula C15H19NO9, where the name is derived from the chromophore 2-methoxy-4-(2-nitrovinyl)phenol and glucoside components.
Biological Activity I Assay Protocols (From Reference)
Targets
There is no specific molecular target reported for MNP-Glc. It functions as a general biochemical reagent, potentially acting as a sulfonylation reagent for organic synthesis and drug discovery.
ln Vitro
There is no specific in vitro activity data available for this compound. As a biochemical reagent, its activity is typically defined by its purity and performance in standard chemical reactions, such as acting as a substrate or intermediate in organic synthesis.
ln Vivo
A specific in vivo activity has not been reported for MNP-Glc. It is classified as a biochemical compound that may be utilized as a biomaterial or organic/chemical reagent for biomedical research, and is not intended for therapeutic in vivo applications.
Enzyme Assay
There is no specific enzyme/receptor binding protocol for this compound. A typical protocol for using MNP-Glc in organic synthesis involves dissolving the compound in an appropriate solvent (e.g., DMF or DMSO) to a desired concentration (e.g., 0.1-1 M) and reacting it with a nucleophile under basic or acidic conditions, with reaction progress monitored by TLC or HPLC.
Cell Assay
There is no specific cell-based protocol for this compound. MNP-Glc is typically utilized as a biochemical reagent rather than a cellular probe. If used in cell culture, a general protocol would involve dissolving the reagent in DMSO to a stock concentration of 10-50 mM, then diluting it to working concentrations (e.g., 1-100 uM) in cell culture medium for specified incubation times.
Animal Protocol
There is no standard in vivo animal protocol for this compound. As a general biochemical reagent, it is not intended for use in live animals.
ADME/Pharmacokinetics
General PK properties for this small molecule are not applicable, as it is not used as a drug. Its stability is typical for a glucoside, and it is likely soluble in polar organic solvents. Formulation is commonly in DMSO for experimental use.
Toxicity/Toxicokinetics
General toxicity for this biochemical reagent is considered low. At working concentrations (uM to mM range), no significant cytotoxicity has been reported. Standard safety precautions for handling laboratory chemicals should be observed.
Additional Infomation
MNP-Glc is a research-grade biochemical reagent intended for laboratory use only. It is available in high purity (typically ≥95%) and is used as a building block in organic synthesis or as a sulfonylation reagent. This product is not for diagnostic or therapeutic use in humans.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C15H19NO9
Molecular Weight
357.31
Exact Mass
357.106
CAS #
70622-80-9
Appearance
Typically exists as solid at room temperature
SMILES
C1(=CC(=CC=C1OC)/C=C/[N+]([O-])=O)O[C@H]1[C@H](O)[C@H]([C@H](O)[C@@H](CO)O1)O
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
Solubility (In Vivo)
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.

Injection Formulations
(e.g. IP/IV/IM/SC)
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution 50 μL Tween 80 850 μL Saline)
*Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution.
Injection Formulation 2: DMSO : PEG300Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO 400 μLPEG300 50 μL Tween 80 450 μL Saline)
Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO 900 μL Corn oil)
Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals).
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Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO 900 μL (20% SBE-β-CD in saline)]
*Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.
Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin 500 μL Saline)
Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO 100 μLPEG300 200 μL castor oil 650 μL Saline)
Injection Formulation 7: Ethanol : Cremophor : Saline = 10: 10 : 80 (i.e. 100 μL Ethanol 100 μL Cremophor 800 μL Saline)
Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline
Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH 900 μL Corn oil)
Injection Formulation 10: EtOH : PEG300Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH 400 μLPEG300 50 μL Tween 80 450 μL Saline)


Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium)
Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose
Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals).
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Oral Formulation 3: Dissolved in PEG400
Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose
Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose
Oral Formulation 6: Mixing with food powders


Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.7987 mL 13.9935 mL 27.9869 mL
5 mM 0.5597 mL 2.7987 mL 5.5974 mL
10 mM 0.2799 mL 1.3993 mL 2.7987 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

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