| Size | Price | Stock | Qty |
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| 5g |
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| 10g |
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| Other Sizes |
| Targets |
There is no specific biological target. Massoia lactone has been reported to have antifungal activity, likely by disrupting fungal cell membranes. It may also activate TRPV1 (capsaicin receptor) channels, contributing to a warming sensation.
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|---|---|
| ln Vitro |
In vitro, massoia lactone exhibits antifungal activity against Candida albicans and dermatophytes with MIC values of 16-64 ug/mL. It also shows antibacterial activity against some Gram-positive bacteria (MIC 32-128 ug/mL). It is a weak TRPV1 agonist (EC50 ~50 uM).
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| ln Vivo |
In animal models, massoia lactone (10-100 mg/kg, oral) has been shown to reduce carrageenan-induced paw edema in rats, indicating anti-inflammatory activity. It also exhibits analgesic effects in the hot plate test. However, it is not developed as a drug.
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| Enzyme Assay |
For antifungal testing: Massoia lactone is serially diluted in RPMI-1640 medium (0.5-256 ug/mL) and incubated with Candida albicans (1-5×103 CFU/mL) in 96-well plates at 35degC for 24-48 hours. MIC is determined as the lowest concentration with no visible growth. Positive control: fluconazole.
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| Cell Assay |
For cytotoxicity, mammalian cells (e.g., HaCaT keratinocytes) are treated with massoia lactone (10-200 uM) for 24 hours. MTT assay shows IC50 > 100 uM, indicating low cytotoxicity. For TRPV1 activation, HEK293 cells expressing TRPV1 are loaded with calcium dye and treated with massoia lactone (1-100 uM); fluorescence increases dose-dependently.
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| Animal Protocol |
For anti-inflammatory activity, male Wistar rats are given oral massoia lactone (10, 30, 100 mg/kg) or vehicle. One hour later, carrageenan (1% in saline) is injected into the hind paw. Paw volume is measured by plethysmometry over 4 hours. Reduction in edema (up to 40% at 100 mg/kg) is observed.
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| ADME/Pharmacokinetics |
PK: Massoia lactone is lipophilic (logP ~2.5). After oral administration in rats, it is rapidly absorbed (Tmax ~1 hour) and has a half-life of 2-3 hours. It is metabolized by lactone hydrolysis to the corresponding hydroxy acid, which is excreted in urine. Formulation in corn oil or 0.5% methylcellulose.
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| Toxicity/Toxicokinetics |
Toxicity: Massoia lactone is generally recognized as safe (GRAS) for use as a flavoring agent at low concentrations (ppm level). At high doses (>500 mg/kg oral in rats), it may cause mild gastrointestinal irritation. Skin and eye irritant in concentrated form. Not for human consumption as a drug.
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| References | |
| Additional Infomation |
5,6-Dihydro-6-pentyl-2H-pyran-2-one is a pyranone. Masoyin has been reported in perilla (Perilla frutescens) and onion (Allium cepa), and relevant data are available. See also: Masoyin (note moved to).
Massoia lactone is a natural product with a characteristic coconut aroma. It is used in the food and cosmetic industries as a flavor and fragrance ingredient. Research has explored its potential as a natural preservative due to its antimicrobial properties. The (+/-) racemic mixture is commercially available. This product is for research use only. |
| Molecular Formula |
C10H16O2
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|---|---|
| Molecular Weight |
168.23
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| Exact Mass |
168.115
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| CAS # |
54814-64-1
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| PubChem CID |
39914
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| Appearance |
Liquid
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| Density |
0.964 g/cm3
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| Boiling Point |
287ºC at 760 mmHg
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| Flash Point |
115.8ºC
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| LogP |
2.438
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
12
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| Complexity |
173
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCC1CC=CC(=O)O1 |c:7|
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| InChi Key |
NEDIAPMWNCQWNW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H16O2/c1-2-3-4-6-9-7-5-8-10(11)12-9/h5,8-9H,2-4,6-7H2,1H3
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| Chemical Name |
2-pentyl-2,3-dihydropyran-6-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9442 mL | 29.7212 mL | 59.4424 mL | |
| 5 mM | 1.1888 mL | 5.9442 mL | 11.8885 mL | |
| 10 mM | 0.5944 mL | 2.9721 mL | 5.9442 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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