| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| 5g |
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| Other Sizes |
| Targets |
2,5-Dioxopyrrolidin-1-yl dodecanoate does not have a defined biological target as a therapeutic agent. It is a chemical reagent used for the acylation of amines in peptide synthesis and bioconjugation. The compound's utility lies in its NHS ester functional group, which reacts with primary amines to form stable amide bonds. It is not intended to exert pharmacological effects.
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| ln Vitro |
Specific in vitro activity data for 2,5-Dioxopyrrolidin-1-yl dodecanoate as a pharmacological agent are not documented in the literature. It is used as a research reagent rather than as a drug substance. The compound is not characterized for direct biological activities such as enzyme inhibition or receptor modulation. Its effects in biological systems are related to its reactivity as an acylating agent.
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| ln Vivo |
2,5-Dioxopyrrolidin-1-yl dodecanoate is not used for in vivo pharmacological activity assessment as a drug compound. Its primary applications are as an activated ester reagent in peptide synthesis and bioconjugation. The compound is not intended to be administered to animals for pharmacological studies. Its use is limited to in vitro chemical synthesis and bioconjugation applications.
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| Enzyme Assay |
In vitro assays for 2,5-Dioxopyrrolidin-1-yl dodecanoate typically involve assessing its reactivity with primary amines. The compound is incubated with amine-containing molecules, and the formation of amide bonds is monitored by HPLC, mass spectrometry, or other analytical methods. Standard bioconjugation protocols are employed for its use, typically in buffered solutions at pH 7.0-8.5.
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| Cell Assay |
2,5-Dioxopyrrolidin-1-yl dodecanoate is used in cell-based assays as a reagent for modifying proteins or peptides that are subsequently tested in cell-based assays. The compound itself is not characterized for direct cellular effects. Its use is limited to the preparation of conjugates for biological studies.
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| Animal Protocol |
2,5-Dioxopyrrolidin-1-yl dodecanoate is not used in animal studies as a pharmacological agent. Its primary applications are as an activated ester reagent in peptide synthesis and bioconjugation. The compound is not intended to be administered to animals for efficacy evaluations. Its use is limited to in vitro chemical synthesis.
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| ADME/Pharmacokinetics |
2,5-Dioxopyrrolidin-1-yl dodecanoate has a molecular weight of 297.39 g/mol and a molecular formula of C₁₆H₂₇NO₄. It is an NHS ester of dodecanoic acid. The compound is typically stored at -20°C, protected from moisture, as the NHS ester is hydrolytically sensitive. It should be allowed to warm to room temperature before opening to prevent moisture condensation.
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| Toxicity/Toxicokinetics |
2,5-Dioxopyrrolidin-1-yl dodecanoate is intended for research use only and is not approved for human therapeutic applications. As a chemical reagent, comprehensive toxicological data are not extensively documented in the publicly accessible literature. The NHS ester may be hydrolytically unstable and should be handled with care. Standard safety precautions should be observed when handling this compound, including the use of appropriate personal protective equipment.
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| Additional Infomation |
2,5-Dioxopyrrolidin-1-yl dodecanoate (CAS#: 14565-47-0) is an NHS ester of dodecanoic acid used as a reagent for the acylation of amines in peptide synthesis and bioconjugation. It reacts with primary amines to form stable amide bonds. This compound is not a drug and is a chemical reagent.
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| Molecular Formula |
C16H27NO4
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|---|---|
| Molecular Weight |
297.39
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| Exact Mass |
297.194
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| CAS # |
14565-47-0
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| PubChem CID |
2802515
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| Appearance |
White to off-white solid powder
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| Density |
1.07g/cm3
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| Boiling Point |
392.3ºC at 760mmHg
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| Flash Point |
191.1ºC
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| Vapour Pressure |
2.31E-06mmHg at 25°C
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| Index of Refraction |
1.489
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| LogP |
3.452
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
21
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| Complexity |
338
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCC(=O)ON1C(=O)CCC1=O
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| InChi Key |
LRKGVVJOUNKTGG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H27NO4/c1-2-3-4-5-6-7-8-9-10-11-16(20)21-17-14(18)12-13-15(17)19/h2-13H2,1H3
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) dodecanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3626 mL | 16.8129 mL | 33.6259 mL | |
| 5 mM | 0.6725 mL | 3.3626 mL | 6.7252 mL | |
| 10 mM | 0.3363 mL | 1.6813 mL | 3.3626 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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